| Budget Amount *help |
¥21,190,000 (Direct Cost: ¥16,300,000、Indirect Cost: ¥4,890,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2018: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
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| Outline of Final Research Achievements |
Most of death from cancer is caused by metastasis. Cancer-stroma interactions are supposed to play critical roles to establish metastasis. To identify and characterize stromal cells interacting with cancer cells in murine metastasis models, we developed a genetically encoded fluorescent labeling technology named sGRAPHIC. sGRAPHIC enables efficient fluorescent labeling of proximal stromal cells interacting with cancer cells in living tissues. sGRAPHIC-labeled stomal cells can be harvested by a fluorescent activated cell sorter. Single cell omics analysis would be powerful to characterize the isolated stromal cells by sGRAPHIC, elucidating cancer-stroma interactions in metastasis.
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