Single cell interactome analysis for the discovery of targets to overcome anti-cancer drug resistance.
| Project/Area Number |
17H04992
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical genome science
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| Research Institution | The University of Tokyo (2018-2019)
Tokyo Medical and Dental University (2017) |
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Principal Investigator |
Komura Daisuke 東京大学, 大学院医学系研究科(医学部), 助教 (10776082)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Fiscal Year 2019: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | がん / 治療標的 / ゲノム / がん-間質細胞間相互作用 / シングルセルトランスクリプトーム / がん間質相互作用 / 癌 |
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| Outline of Final Research Achievements |
From single-cell transcriptome data of tumor tissues, we developed a method to analyze and visualize the entire cell-cell interaction (interactome) between various cells such as cancer cells, fibroblasts, immune cells, and vascular endothelial cells. Using this method, we performed interactome analysis of single-cell transcriptome data of normal gastric mucosa and gastric cancer tissues from human samples, and gastric cancer tissues from the mouse model of diffuse-type gastric cancer. The results suggest that signals from various cells, including fibroblasts, may contribute to the progression of cancer. These included molecules that have been reported to be associated with attenuated effects of anticancer drugs.
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| Academic Significance and Societal Importance of the Research Achievements |
がん細胞は通常それ単独では生存できず、周囲のさまざまな細胞と相互作用することによって生存に有利な状況を作り出している。近年組織における遺伝子発現量を一細胞レベルで定量化する技術が開発された。本研究ではこのデータを用いてがん細胞と周囲の非がん細胞との間の相互作用を定量化・可視化する技術を開発した。本手法をマウスのがん組織やヒトの正常組織、がん組織に適用したところ、正常組織からがん組織に至る過程で重要な相互作用を明らかにした。また、その中には抗がん剤の効果を弱める可能性のある分子も含まれていた。本研究成果をさらに進めることでがんの本体解明や抗癌剤耐性獲得の機序解明などにつながることが期待される。
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Novel targets identified by integrated cancer-stromal interactome analysis of pancreatic adenocarcinoma.2020
Author(s)
Hiroshima Y, Kasajima R, Kimura Y, Komura D, Ishikawa S, Ichikawa Y, Bouvet M, Yamamoto N, Oshima T, Morinaga S, Singh SR, Hoffman RM, Endo I, Miyagi Y.
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Journal Title
Cancer Lett.
Volume: 469
Pages: 217-227
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] High expression of olfactomedin-4 is correlated with chemoresistance and poor prognosis in pancreatic cancer.2020
Author(s)
Ohkuma R, Yada E, Ishikawa S, Komura D, Ishizaki H, Tamada K, Kubota Y, Hamada K, Ishida H, Hirasawa Y, Ariizumi H, Satoh E, Shida M, Watanabe M, Onoue R, Ando K, Tsurutani J, Yoshimura K, Yokobori T, Sasada T, Aoki T, Murakami M, Norose T, Ohike N, Takimoto M, Izumizaki M, Kobayashi S, Tsunoda T, Wada S.
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Journal Title
PLoS One
Volume: 10
Issue: 1
Pages: e0226707-e0226707
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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