Analysis of neuronal mechanisms for flight control

• Project/Area Number: 17H05011
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Animal physiology/Animal behavior
• Research Institution: The University of Tokyo
• Principal Investigator: Namiki Shigehiro 東京大学, 先端科学技術研究センター, 准教授 (40567757)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000); Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
• Keywords: はばたき / 神経行動学 / 光遺伝学 / 飛翔神経叢 / Split-GAL4 / ハルテア神経叢 / 神経回路 / 飛行回路 / Spllit-GAL4 / ニューロンデータベース / 飛行制御 / 飛翔 / コマンド / 飛行ニューロン / GAL4 / ショウジョウバエ / コネクトーム / 飛翔筋 / 下行性ニューロン / 小型飛翔体

Outline of Final Research Achievements

In animals, behavior is controlled by a group of descending neurons that connect the brain to the body. In this study, we focused on DNg02, descending neuron that have been shown to play an important role in flight control in Drosophila, and analyzed it in detail using molecular genetics, optogenetics, optophysiology, and neuroanatomy. DNg02 is a population of cells with similar morphological features to each other, each with the ability to drive flapping motion. The intensity of flapping increased with the number of active cells, suggesting that DNg02 encodes flapping commands collectively.

Academic Significance and Societal Importance of the Research Achievements

本研究は脳から身体へ発し、飛行を司令する細胞を初めての報告となる。昆虫は神経行動学の重要なモデル生物であるが、これまでの事例からは単一の下行性ニューロンが単一の行動を駆動するという考え方が一般的であった。本研究では飛行という行動が複数のニューロンによって制御されていることを示した点において、学術的な意義がある。また本研究において開発した神経細胞を選択的に標識する遺伝学的リソースは、神経科学研究のために有用であり、今後研究コミュニティに広く用いられると期待される。

Research Products

• [Int'l Joint Research] Howard Hughes Medical Institute/California Institute of Technology(米国)
• [Int'l Joint Research] Howard Hughes Medical Institute/Janelia Research Campus(米国)
• [Journal Article] A Systematic Nomenclature for the Drosophila Ventral Nerve Cord. (2020)
  • Author(s): Court R, Namiki S, Armstrong D, Borner J, Card G, Costa M, Dickinson MH, Duch C, Korff W, Mann R, Merritt D, Murphey R, Seeds A, Shirangi T, Simpson J, Truman J, Tuthill J, Williams D, Shepherd D
  • Journal Title: Neuron Volume: 107 Issue: 6 Pages: 1071-1079
  • DOI: 10.1016/j.neuron.2020.08.005
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Neural circuit basis of aversive odour processing in Drosophila from sensory input to descending output (2020)
  • Author(s): Huoviala P, Dolan MJ, Love FM, Myers P, Frechter S, Namiki S, Pettersson L, Roberts RJV, Turnbull R, Mitrevica Z, Breads P, Schlegel P, Bates AS, Rodrigues T, Aso Y, Bock D, Rubin GM, Stensmyr M, Card G, Costa M, Jefferis GSXE
  • Journal Title: bioRxiv Volume: 394403 Pages: 1-16
  • DOI: 10.1101/394403
  • Open Access / Int'l Joint Research
• [Journal Article] An image resource of subdivided Drosophila GAL4-driver expression patterns for neuron-level searches (2020)
  • Author(s): Meissner GW, Dorman Z, Nern A, Forster K, Gibney T, Jeter J, Lohnson L, He Y, Lee K, Melton B, Yarbrough B, Clements J, Goina C, Otsuna H, Rokicki K, Svirskas RR, Aso Y, Card GM, Dickson BJ, Ehrhardt E, Goldammer J, Ito M, Korff W, Minegishi R, Namiki S, Rubin GM, Sterne G, Wolff T, Malksman O
  • Journal Title: bioRxiv Volume: 080473 Pages: 1-16
  • DOI: 10.1101/2020.05.29.080473
  • Open Access / Int'l Joint Research
• [Journal Article] State-dependent decoupling of sensory and motor circuits underlies behavioral flexibility in Drosophila (2019)
  • Author(s): Ache Jan M.、Namiki Shigehiro、Lee Allen、Branson Kristin、Card Gwyneth M.
  • Journal Title: Nature Neuroscience Volume: 22 Issue: 7 Pages: 1132-1139
  • DOI: 10.1038/s41593-019-0413-4
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Morphology and physiology of olfactory neurons in the lateral protocerebrum of the silkmoth Bombyx mori (2019)
  • Author(s): Namiki Shigehiro、Kanzaki Ryohei
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 1-14
  • DOI: 10.1038/s41598-019-53318-8
  • Peer Reviewed / Open Access
• [Journal Article] The functional organization of descending sensory-motor pathways in Drosophila (2018)
  • Author(s): Namiki Shigehiro、Dickinson Michael H、Wong Allan M、Korff Wyatt、Card Gwyneth M
  • Journal Title: eLife Volume: 7 Pages: 1-50
  • DOI: 10.7554/elife.34272
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Optogenetic dissection of descending behavioral control in Drosophila (2018)
  • Author(s): Cande Jessica、Namiki Shigehiro、Qiu Jirui、Korff Wyatt、Card Gwyneth M、Shaevitz Joshua W、Stern David L、Berman Gordon J
  • Journal Title: eLife Volume: 7 Pages: 1-23
  • DOI: 10.7554/elife.34275
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Speed dependent descending control of freezing behavior in Drosophila melanogaster (2018)
  • Author(s): Zacarias Ricardo、Namiki Shigehiro、Card Gwyneth M.、Vasconcelos Maria Luisa、Moita Marta A.
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 1-11
  • DOI: 10.1038/s41467-018-05875-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Descending neurons from the lateral accessory lobe and posterior slope in the brain of the silkmoth Bombyx mori (2018)
  • Author(s): Namiki Shigehiro、Wada Satoshi、Kanzaki Ryohei
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 1-19
  • DOI: 10.1038/s41598-018-27954-5
  • Peer Reviewed / Open Access
• [Journal Article] Feature Integration Drives Probabilistic Behavior in the Drosophila Escape Response (2017)
  • Author(s): von Reyn Catherine R.、Nern Aljoscha、Williamson W. Ryan、Breads Patrick、Wu Ming、Namiki Shigehiro、Card Gwyneth M.
  • Journal Title: Neuron Volume: 94 Issue: 6 Pages: 1190-1204.e6
  • DOI: 10.1016/j.neuron.2017.05.036
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A Systematic Nomenclature for the DrosophilaVentral Nervous System (2017)
  • Author(s): Court Robert Christopher、Armstrong James Douglas、Borner Jana、Card Gwyneth、Costa Marta、Dickinson Michael、Duch Carsten、Korff Wyatt、Mann Richard、Merritt David、Murphey Rod、Namiki Shigehiro、Seeds Andrew、Shepherd David、Shirangi Troy、Simpson Julie、Truman James、Tuthill John、Williams Darren
  • Journal Title: bioRxiv Volume: N/A Pages: 1-42
  • DOI: 10.1101/122952
  • Open Access / Int'l Joint Research
• [Journal Article] Optogenetic dissection of descending behavioral control in Drosophila (2017)
  • Author(s): Cande Jessica、Berman Gordon J、Namiki Shigehiro、Qiu Jirui、Korff Wyatt、Card Gwyneth、Shaevitz Joshua W、Stern David L
  • Journal Title: bioRxiv Volume: N/A Pages: 1-50
  • DOI: 10.1101/230128
  • Open Access / Int'l Joint Research
• [Journal Article] The functional organization of descending sensory-motor pathways in Drosophila (2017)
  • Author(s): Namiki Shigehiro、Dickinson Michael Hughes、Wong Allan M、Korff Wyatt、Card Gwyneth M
  • Journal Title: bioRxiv Volume: N/A Pages: 1-67
  • DOI: 10.1101/231696
  • Open Access / Int'l Joint Research
• [Journal Article] Speed dependent descending control of innate freezing behavior inDrosophila melanogaster (2017)
  • Author(s): Zacarias Ricardo、Namiki Shigehiro、Card Gwyneth、Vasconcelos Maria L、Moita Marta A
  • Journal Title: bioRxiv Volume: N/A Pages: 1-27
  • DOI: 10.1101/234443
  • Open Access / Int'l Joint Research
• [Journal Article] The morphology of antennal lobe projection neurons is controlled by a POU-domain transcription factor Bmacj6 in the silkmoth Bombyx mori (2017)
  • Author(s): Namiki S, Fujii T, Shimada T, Kanzaki R
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 14050-14050
  • DOI: 10.1038/s41598-017-14578-4
  • Peer Reviewed / Open Access
• [Journal Article] Morphology and physiology of antennal lobe projection neurons in the hawkmoth Agrius convolvuli (2017)
  • Author(s): Nirazawa T, Fujii T, Seki Y, Namiki S, Kazawa T, Kanzaki R, Ishikawa Y
  • Journal Title: Journal of Insect Physiology Volume: 98 Pages: 214-222
  • DOI: 10.1016/j.jinsphys.2017.01.010
  • Peer Reviewed
• [Presentation] ハエの飛行を司令する神経細胞の探索 (2020)
  • Author(s): 並木重宏
  • Organizer: 第42回エアロ・アクアバイオメカニズム定例講演会
  • Invited
• [Presentation] Descending control of landing in Drosophila (2019)
  • Author(s): Ache JM, Namiki S, Lee A, Branson K, Card GM
  • Organizer: The Society for Integrative & Comparative Biology Annual Meeting 2019
  • Int'l Joint Research
• [Presentation] Descending control of flight behavior in flies (2019)
  • Author(s): Namiki S, Ros I, Rowell W, de Souza A, Dickinson MH, Korff WL, Card GM
  • Organizer: The Society for Integrative & Comparative Biology Annual Meeting 2019,
  • Int'l Joint Research
• [Presentation] Feature detection and action selection in neuronal circuits for escape and and landing Drosophila. (2019)
  • Author(s): Ache JM, Namiki S, von Reyn CR, Card GM
  • Organizer: 13th Gottingen Meeting of the German Neuroscience Society
  • Int'l Joint Research
• [Presentation] A cell-type specific driver line library targeting motoneurons and interneurosn in the wing neuropil of the ventral nerve cord of Drosophila melanogaster (2019)
  • Author(s): Ehrchardt E, Whitehead S, Namiki S, Siwanowicz I, Otsuna H, Stern D, Dickinson M, Ito K, Truman J, Cohen I, Korff W, Card GM
  • Organizer: 13th Gottingen Meeting of the German Neuroscience Society
  • Int'l Joint Research
• [Presentation] 昆虫の行動司令を担う神経回路の分析 (2019)
  • Author(s): 並木重宏
  • Organizer: 第90回日本動物学会大会奨励賞受賞者講演
  • Invited
• [Presentation] Enlarged macroglomerular complex in moths with elaborate antenna (2019)
  • Author(s): Namiki S, Shiota Y, Nirazawa T, Fujii T, Uehara T, Nakano R, Naka H, Kanzaki R
  • Organizer: 第41回日本比較生理生化学会大会
• [Presentation] Descending control of landing in Drosophila (2019)
  • Author(s): Ache JM, Namiki S, Lee A, Branson K, Card GM
  • Organizer: The Society for Integrative & Comparative Biology Annual Meeting 2019, Tampa, FL, USA
  • Int'l Joint Research
• [Presentation] Descending control of flight behavior in flies (2019)
  • Author(s): Namiki S, Ros I, Rowell W, de Souza A, Dickinson MH, Korff WL, Card GM
  • Organizer: The Society for Integrative & Comparative Biology Annual Meeting 2019, Tampa, FL, USA
  • Int'l Joint Research
• [Presentation] Ache JM, Namiki S, von Reyn CR, Card GM (2019)
  • Author(s): Feature detection and action selection in neuronal circuits for escape and and landing Drosophila
  • Organizer: 13th Gottingen Meeting of the German Neuroscience Society, Centre for Humanities, Gottingen, Germany
  • Int'l Joint Research
• [Presentation] A cell-type specific driver line library targeting motoneurons and interneurosn in the wing neuropil of the ventral nerve cord of Drosophila melanogaster (2019)
  • Author(s): Ehrchardt E, Whitehead S, Namiki S, Siwanowicz I, Otsuna H, Stern D, Dickinson M, Ito K, Truman J, Cohen I, Korff W, Card GM
  • Organizer: 13th Gottingen Meeting of the German Neuroscience Society, Centre for Humanities, Gottingen, Germany
  • Int'l Joint Research
• [Presentation] 昆虫の行動司令を担う神経回路の分析 (2019)
  • Author(s): 並木重宏
  • Organizer: 第90回日本動物学会大会奨励賞受賞者講演，大阪市立大学，大阪
  • Int'l Joint Research / Invited
• [Presentation] The functional organization of descending sensory-motor pathways in Drosophila (2018)
  • Author(s): Namiki S, Dickinson MH, Wong A, Korff W, Card GM
  • Organizer: Janelia Conference "Neural Circuits of the Insect Ventral Nerve Cord", HHMI Janelia Research Campus, VA USA
  • Int'l Joint Research / Invited
• [Presentation] Innate freezing behavior, triggered by P9 descending neurons, is plastic (2018)
  • Author(s): Zacarias R, Ferreira C, Vieira R, Namiki S, Card G, Vasconcelos ML, Moita MA
  • Organizer: Janelia Conference "Neural Circuits of the Insect Ventral Nerve Cord", HHMI Janelia Research Campus, VA USA
  • Int'l Joint Research / Invited
• [Presentation] Optogenetic activation of wing motoneurons of Drosophila melanogaster in flight and courtship song (2018)
  • Author(s): Ehrhardt EE, Namiki S, Whitehead S, Truman J, Dickinson M, Cohen I, Stern D, Korff W, Card GM
  • Organizer: Janelia Conference "Neural Circuits of the Insect Ventral Nerve Cord", HHMI Janelia Research Campus, VA USA
  • Int'l Joint Research
• [Presentation] Context-dependent control of landing in Drosophila (2018)
  • Author(s): Ache JM, Rogers E, Namiki S, Lee A, Branson K, Card GM
  • Organizer: Janelia Conference "Neural Circuits of the Insect Ventral Nerve Cord", HHMI Janelia Research Campus, VA USA
  • Int'l Joint Research
• [Presentation] 下行性神経細胞群によるはばたきの制御 (2018)
  • Author(s): 並木重宏
  • Organizer: 第40回日本比較生理生化学会大会シンポジウム「運動におけるリズムとタイミング制御の比較生理学」，神戸大学先端融合研究環統合研究拠点コンベンションホール ，神戸
  • Int'l Joint Research / Invited
• [Presentation] エビガラスズメにおける近縁種の性フェロモンの情報処理機構 (2018)
  • Author(s): 韮澤拓也，並木重宏，関洋一，藤井毅，石川幸男，加沢知毅，神崎亮平
  • Organizer: 第 61回日本応用動物昆虫学会大会
• [Presentation] A cell type specific driver line library targeting wing motor circuits in the ventral nerve cord of Drosophila (2017)
  • Author(s): Ehrhardt EE, Namiki S, Otsuna H, Cohen I, Stern D, Dickinson M, Korff W, Card GM
  • Organizer: Neuroscience 2017, Society for Neuroscience
  • Int'l Joint Research
• [Presentation] Control of landing in Drosophila (2017)
  • Author(s): Ache JM, Namiki S, Card GM
  • Organizer: Neuroscience 2017, Society for Neuroscience
  • Int'l Joint Research
• [Presentation] Descending interneurons controlling visually-evoked escape in Drosophila (2017)
  • Author(s): Peek M, Namiki S, Breads P, Williamson WR, Ache JM, Card GM
  • Organizer: Neuroscience 2017, Society for Neuroscience
  • Int'l Joint Research
• [Presentation] ガ類のフェロモン選好性を決める神経回路 (2017)
  • Author(s): 並木重宏，神崎亮平
  • Organizer: 日本進化学会第19回大会シンポジウム「繁殖行動の進化をもたらす神経基盤」
  • Invited
• [Presentation] Comparative Neuroscience Platform: the environment for the comparative neuroscience by facilitating data analysis and collaborations through neuroinformatics (2017)
  • Author(s): Kazawa T, Miyamoto D, Seki Y, Yosida M, Iwatsuki C, Ando N, Namiki S, Ikeno H, Nishino H, Oota S, Murata, Kanazawa A, Ichiishi H, Kanamoto Y, Yamaguchi Y, Kanzaki R
  • Organizer: Advances in Neuroinformatics 2017
• [Presentation] Developing comparative neuroscience platform (CNS-PF): the environment for facilitating data analysis and collaborations on comparative neuroinformatics (2017)
  • Author(s): Kazawa T, Miyamoto D, Seki Y, Yoshida M, Iwatsuki C, Ando N, Namiki S, Ikeno H, Nishino H, Oota S, Murata Y, Kanazawa A, Ichiishi H, Kanamoto Y, Yamaguchi Y, Kanzaki R
  • Organizer: The 39th Annual Meeting of the Japanese Society for Comparative Physiology and Biochemistry
• [Presentation] Difference of the neural pathways between the sex pheromone and behavioral antagonist in the hawkmoth, Agrius conbolvuili (2017)
  • Author(s): Nirazawa T，Namiki S, Seki Y, Fujii T, Ishikawa Y, Kazawa T, Kanzaki R
  • Organizer: 日本比較生理科学会第 39 回福 岡大会
• [Book] 昆虫の脳をつくる (2018)
  • Author(s): 神崎亮平
  • Total Pages: 224
  • Publisher: 朝倉書店
  • ISBN: 9784254102772
• [Book] 動物学の百科事典 (2018)
  • Author(s): 公益社団法人日本動物学会
  • Total Pages: 800
  • Publisher: 丸善出版
  • ISBN: 9784621303092
• [Remarks] Fly Descending Interneuron Project
  • URL: https://www.janelia.org/project-team/fly-descending-interneuron