A study on an ancestral sulfur transfer pathway

• Project/Area Number: 17H05027
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Kobe University (2018-2019); Kwansei Gakuin University (2017)
• Principal Investigator: HIDESE RYOTA 神戸大学, 科学技術イノベーション研究科, 特命准教授 (90610866)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2018: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000); Fiscal Year 2017: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
• Keywords: ユビキチン様タンパク質 / 好熱性アーキア / 硫黄転移系 / モリブドプテリン / tRNA硫黄化修飾 / 原始生命体 / tRNAチオ化修飾 / tRNA硫黄化 / 超好熱性アーキア / 元素硫黄 / アーキア / 生命の起源 / 微生物代謝 / 超好熱菌 / 硫黄転移 / 原始生命

Outline of Final Research Achievements

I analyzed the physiological roles of ubiquitine-like proteins as sulfur carriers for sulfur-containing biofactors in a hyperthermophilic archaeon Thermococcus kodakarensis. The ubl less-strain showed the decreased activities of molybodopterin-containing enzymes and thiolation rate of tRNA thionucleoside, indicating Ubl would be involved in the biosynthesis as sulfur-carrier; on the other hand, Ubls are dispensable for the biosyntheses of sulfur-containing biomolecules in the cells grown in the presence of elemental sulfur.

Academic Significance and Societal Importance of the Research Achievements

本研究で、T. kodakarensisはUbl硫黄転移系を介して含硫化合物を合成する機構とUbl転移系を介さず外環境の元素硫黄により直接的に制御される２つの硫黄化機構を有することを示す結果を得た。モリブドプテリンやtRNAチオヌクレオシドは全ての生物に共通して存在する根源的な生体化合物である。元素硫黄などの還元硫黄こそが原始生命体が有していた始原的代謝経路の硫黄ドナーであることを示唆した点は、生命の起源を考察する上で学術的意義が極めて高い。

Research Products

• [Journal Article] Branched-chain polyamine stabilizes RNA polymerase at elevated temperatures in hyperthermophiles. (2020)
  • Author(s): Yamori Y, Hamakawa M, Hidese R, Fukuda M, Atomi H, Fukuda W, Fujiwara S.
  • Journal Title: Amino Acids Volume: 52 Issue: 2 Pages: 275-285
  • DOI: 10.1007/s00726-019-02745-y
  • Peer Reviewed
• [Journal Article] Genes Regulated by Branched-Chain Polyamine in the Hyperthermophilic Archaeon Thermococcus Kodakarensis. (2020)
  • Author(s): Fukuda W, Yamori Y, Hamakawa M, Osaki M, Fukuda M, Hidese R, Kanesaki Y, Okamoto-Kainuma A, Kato S, Fujiwara S.
  • Journal Title: Amino Acids Volume: 52 Issue: 2 Pages: 287-299
  • DOI: 10.1007/s00726-019-02793-4
  • Peer Reviewed
• [Journal Article] The C‐terminal flexible region of branched‐chain polyamine synthase facilitates substrate specificity and catalysis (2019)
  • Author(s): Ryota Hidese, Masataka Toyoda, Ken‐ichi Yoshino, Wakao Fukuda , Gita Adhirani Wihardja, Seigo Kimura, Junso Fujita, Masaru Niitsu, Tairo Oshima, Tadayuki Imanaka, Eiichi Mizohata, and Shinsuke Fujiwara
  • Journal Title: The FEBS Journal Volume: 286 Issue: 19 Pages: 3926-3940
  • DOI: 10.1111/febs.14949
  • Peer Reviewed
• [Journal Article] Thermostable DNA helicase improves the sensitivity of digital PCR (2018)
  • Author(s): Hidese, R., Kawato, K., Nakura, Y., Yasukawa, K., Yanagihara, I., Fujiwara, S.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 495 Issue: 3 Pages: 2189-2194
  • DOI: 10.1016/j.bbrc.2017.12.053
  • Peer Reviewed
• [Journal Article] Identification of a novel acetylated form of branched-chain polyamine from a hyperthermophilic archaeon Thermococcus kodakarensis (2018)
  • Author(s): Hidese, R., Im, KH., Kobayashi, M., Niitsu, M., Furuchi, T., Fujiwara, S.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 81 Issue: 9 Pages: 1845-1849
  • DOI: 10.1080/09168451.2017.1345616
  • Peer Reviewed
• [Journal Article] Active site geometry of a novel aminopropyltransferase for biosynthesis of hyperthermophile-specific branched-chain polyamine (2017)
  • Author(s): R. Hidese, K. M. Tse, S. Kimura, E. Mizohata, J. Fujita, Y. Horai, N. Umezawa, T. Higuchi, M. Niitsu, T. Oshima, T. Imanaka, T. Inoue, and S. Fujiwara
  • Journal Title: The FEBS Journal Volume: 284 Issue: 21 Pages: 3684-3701
  • DOI: 10.1111/febs.14262
  • Peer Reviewed / Open Access
• [Journal Article] Gene regulation of two ferredoxin:NADP+ oxidoreductases by the redox-responsive regulator SurR in Thermococcus kodakarensis. (2017)
  • Author(s): Hidese R, Yamashita K, Kawazuma K, Kanai T, Atomi H, Imanaka T, Fujiwara S.
  • Journal Title: Extremophiles Volume: 21 Issue: 5 Pages: 903-917
  • DOI: 10.1007/s00792-017-0952-0
  • Peer Reviewed
• [Presentation] Physiological roles of Thermococcus kodakarensis ubiquitin-like proteins in the biosynthesis of sulfur-containing biomolecules. (2019)
  • Author(s): Hidese R, Sakakibara S, Ohira T, Suzuki T, Shigi N, Fujiwara S
  • Organizer: Thermophiles2019
  • Int'l Joint Research
• [Presentation] 超好熱性アーキアのモリブドプテリン生合成における硫黄転移系 (2018)
  • Author(s): 秀瀬 涼太
  • Organizer: 第65回日本生化学会近畿支部例会
• [Presentation] 硫黄転移系から紐解く初期生命の進化 (2018)
  • Author(s): 秀瀬 涼太
  • Organizer: 第19回極限環境生物学会年会
  • Invited
• [Presentation] 超好熱性アーキアにおけるユビキチン様タンパク質の機能解析 (2018)
  • Author(s): 榊原 早津季, 秀瀬 涼太, 福田 青郎, 藤原 伸介
  • Organizer: 第70回日本生物工学会大会
• [Presentation] 超好熱性アーキアの硫黄転移に関わるユビキチン様タンパク質の同定 (2018)
  • Author(s): 秀瀬涼太、榊原早津季、藤原伸介
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] 超好熱性アーキアの含硫化合物への硫黄転移に関わるユビキチン様タンパク質の同定 (2018)
  • Author(s): 秀瀬涼太、榊原早津季、藤原伸介
  • Organizer: 日本農芸化学会 関西・中四国・西日本支部 2017年度合同大阪大会