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Nutrition-detoxification complex metabolism in lepidopteran larval midgut

Research Project

Project/Area Number 17H05029
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Bioorganic chemistry
Research InstitutionKyoto University

Principal Investigator

Yoshinaga Naoko  京都大学, 農学研究科, 助教 (40456819)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords鱗翅目幼虫 / 窒素代謝 / 解毒 / FACs / iridoids / L-ACY-1 / Spodoptera litura / SiRNAi / ゲノム編集 / イリドイド / gardenoside / β-alanine / 配糖体 / β-アラニン / 中腸 / 鱗翅目 / 栄養代謝
Outline of Final Research Achievements

FACs, fatty acid-amino acid condensates in the gut of lepidopteran larvae, are involved in efficient nitrogen metabolism in larvae. The FACs biosynthetic enzymes in Spodoptera litura larvae are lepidopteran-specific carboxylesterases with unknown functions. By genome editing of the same species, we succeeded in generating a line lacking the FACs-degrading enzyme, L-ACY-1. These lines showed delayed growth compared to the wild type, and also their detoxification ability seemed to be reduced. The larvae neutralize gardenoside, a toxic substance found in gardenia leaves, by accumulating a large amount of β-alanine in the intestine. We found the induction was triggered by the glycoside glucose. The complex cross-link between nutrient metabolism and detoxification metabolism in the larval body was revealed.

Academic Significance and Societal Importance of the Research Achievements

鱗翅目幼虫は特殊な窒素代謝系を獲得しており、窒素栄養価の低い植物を餌としながら窒素同化を効率的に行う。特に農業害虫では、棘や毒、営巣といった天敵に対する防御を一切せず、成長速度を最大化して、高密度に繁殖することで生存戦略とする種が多数見られる。この戦略では、食餌から吸収した栄養を成長・繁殖にどこまで投入できるかが鍵となっており、制約となりうるのが解毒にかかるコストである。この観点から窒素代謝と解毒代謝の直接的な関係を明らかにした研究は少なく、今後の植物保護とペストコントロールに重要な知見となった。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (20 results)

All 2020 2019 2018 2017 Other

All Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Inhibitory substances contained in calcium carbonate wettable powder on the oviposition of the peach fruit moth, <i>Carposina sasakii</i>2020

    • Author(s)
      Kazama H, Oohata Y, Takanashi T, Tokoro M, Ishiguri Y, Mori N, Yoshinaga N
    • Journal Title

      Journal of Pesticide Science

      Volume: 45 Issue: 1 Pages: 16-23

    • DOI

      10.1584/jpestics.D19-066

    • NAID

      130007800771

    • ISSN
      1348-589X, 1349-0923
    • Year and Date
      2020-02-20
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 鱗翅目幼虫由来エリシターFACsをめぐる植物-昆虫の対抗適応戦略2020

    • Author(s)
      吉永直子
    • Journal Title

      香料

      Volume: 288 Pages: 21-26

    • NAID

      40022447003

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Variation in Bufadienolide Composition of Parotoid Gland Secretion From Three Taxa of Japanese Toads2020

    • Author(s)
      Inoue Takato、Nakata Ryu、Savitzky Alan H.、Yoshinaga Naoko、Mori Akira、Mori Naoki
    • Journal Title

      Journal of Chemical Ecology

      Volume: 46 Issue: 10 Pages: 997-1009

    • DOI

      10.1007/s10886-020-01217-y

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dramatic dietary shift maintains sequestered toxins in chemically defended snakes2020

    • Author(s)
      Tatsuya Yoshida, Rinako Ujiie, Alan H. Savitzky, Teppei Jono, Takato Inoue, Naoko Yoshinaga, Shunsuke Aburaya, Wataru Aoki, Hirohiko Takeuchi, Li Ding, Qin Chen, Chengquan Cao, Tein-Shun Tsai, Anslem de Silva, Dharshani Mahaulpatha, Tao Thien Nguyen, Yezhong Tang, Naoki Mori, and Akira Mori
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 117 Issue: 11 Pages: 5964-5969

    • DOI

      10.1073/pnas.1919065117

    • NAID

      120006800720

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A rapid, easy and inexpensive method for the quantification of chitin in insect peritrophic membrane by image processing2019

    • Author(s)
      Nakata N, Yoshinaga N, Teraishi M, Okumoto Y, Mori N
    • Journal Title

      Biosci. Biothechnol. Biochem

      Volume: 83 Issue: 9 Pages: 1624-1629

    • DOI

      10.1080/09168451.2019.1611407

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 植物の揮発成分誘導応答に見る昆虫-植物間の化学戦略2019

    • Author(s)
      吉永直子
    • Journal Title

      AROMA RESEARCH

      Volume: 78

    • Related Report
      2018 Annual Research Report
  • [Journal Article] A fragmentation study of isoflavones by LC-IT-TOF-MS using biosynthesized isotopes2018

    • Author(s)
      Nakata, Ryu; YOSHINAGA, Naoko; Teraishi, Masayoshi; Okumoto, Yutaka; Huffaker, Alisa; Schmelz, Eric; MORI, Naoki
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 植物イリドイドをめぐるチョウ目昆虫の様々な戦略2019

    • Author(s)
      吉永直子
    • Organizer
      日本応用動物昆虫学会公開シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 昆虫由来エリシターFACsの生合成酵素の探索2019

    • Author(s)
      野村泰介、義本裕介、宮下正弘、吉永直子、森直樹
    • Organizer
      日本農芸化学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] クチナシ毒 gardenoside に対するハスモンヨトウ幼虫の解毒応答メカニズム2019

    • Author(s)
      吉岡英晃、岩井崇晃、吉永直子、森直樹
    • Organizer
      日本農芸化学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] クチナシ由来イリドイドをめぐる鱗翅目昆虫の解毒戦略2018

    • Author(s)
      吉永 直子、志野 真実子、吉岡 英晃、岩井 崇晃、内藤 裕彬、齊藤 準、森 直樹
    • Organizer
      日本分子生物学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 昆虫-植物間の化学的相克を活かした 次世代型農業害虫管理2018

    • Author(s)
      吉永直子
    • Organizer
      農学進歩賞受賞講演
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] モモシンクイガに対する炭酸カルシウム水和剤の産卵阻害活性2018

    • Author(s)
      風間春奈1, 高梨琢磨2, 所雅彦2, 石栗陽一3, 森直樹1, 吉永直子1
    • Organizer
      日本農芸化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] リンゴの傷害応答性トリテルペン類縁体の経時変化2018

    • Author(s)
      大畑勇統,森田沙代,吉永直子,石栗陽一,森直樹
    • Organizer
      日本農芸化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Effect of inhibitory substances contained in calcium carbonate wettable powder on oviposition of peach fruit moth, Carposina sasakii (Lepidoptera: Carposinidae)2017

    • Author(s)
      Haruna Kazama1*, Takuma Takanashi2, Masahiko Tokoro2, Yoichi Ishiguri3, Naoki Mori1, Naoko Yoshinaga1
    • Organizer
      International Society of Chemical Ecology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification and investigation of compounds induced in apple fruits by insect attack2017

    • Author(s)
      Yuuki Tetsumoto1*, Naoko Yoshinaga1, Yoichi Ishiguri2*, Naoki Mori1
    • Organizer
      International Society of Chemical Ecology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Book] Chemical Ecology of Insects : Applications and Associations with Plants and Microbes2018

    • Author(s)
      田端純(編)
    • Total Pages
      296
    • Publisher
      CRC Pr I Llc
    • ISBN
      9781498769402
    • Related Report
      2017 Annual Research Report
  • [Remarks] 京都大学農学研究科化学生態学分野

    • URL

      http://www.chemeco.kais.kyoto-u.ac.jp/index.html

    • Related Report
      2020 Annual Research Report
  • [Remarks] 京都大学農学研究科 化学生態学分野

    • URL

      http://www.chemeco.kais.kyoto-u.ac.jp/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 京都大学 化学生態学研究室

    • URL

      http://www.chemeco.kais.kyoto-u.ac.jp/

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2022-01-27  

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