| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
FACs, fatty acid-amino acid condensates in the gut of lepidopteran larvae, are involved in efficient nitrogen metabolism in larvae. The FACs biosynthetic enzymes in Spodoptera litura larvae are lepidopteran-specific carboxylesterases with unknown functions. By genome editing of the same species, we succeeded in generating a line lacking the FACs-degrading enzyme, L-ACY-1. These lines showed delayed growth compared to the wild type, and also their detoxification ability seemed to be reduced. The larvae neutralize gardenoside, a toxic substance found in gardenia leaves, by accumulating a large amount of β-alanine in the intestine. We found the induction was triggered by the glycoside glucose. The complex cross-link between nutrient metabolism and detoxification metabolism in the larval body was revealed.
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