| Budget Amount *help |
¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2019: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2018: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2017: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
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| Outline of Final Research Achievements |
Embryonic diapause in the silkworm, Bombyx mori, is regulated by temperature and photoperiodic conditions during the preceding generation, but the molecular mechanism is not fully elucidated. The goal of this study is to get the whole picture of the embryonic diapause regulation. We have identified a gene that is key to understand the molecular mechanism. The silkmoths which lost the key gene produce non-diapause eggs under diapause-inducing conditions. The key gene controls a system together with several genes. In this study, CRISPR/Cas9-mediated gene editing was performed in these several genes. Most of the knockout silkmoths as well as the key gene knockout laid non-diapause eggs under diapause-inducing conditions, indicating that the system itself regulates the embryonic diapause. Furthermore, transcriptome analyses identified a group of genes whose expression was altered when a few diapause-inducing genes were disrupted by the gene editing.
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