| Budget Amount *help |
¥24,700,000 (Direct Cost: ¥19,000,000、Indirect Cost: ¥5,700,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
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| Outline of Final Research Achievements |
In this study, we carried genome mining based on the polyketide synthase (PKS) gene of filamentous fungi and explored gene or gene clusters of unprecedented PKS genes, and heterologously expressed them in Aspergillus oryzae to produce natural products with novel structure and biological activities. As a result, we succeeded in discovering a PKS that shows the maximum number of repetitions as a filamentous fungus iterative PKS and a new polyene macrolide. We also succeeded in discovering new antibacterial active macrolides with phoscocholine and phosphoethanolamine, and characterized the enzymes that added them to macrolide skeleton. The results of this research will accelerate the discovery of new natural products that will be useful in drug development in the future.
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