| Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2019: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2018: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Outline of Final Research Achievements |
In order to analyze the detailed mechanism of SREBP-1c gene regulation in vivo, which is important for the regulation of lipid synthesis in liver, I developed the cis sequence identification method using adenovirus reporter vector and in vivo imaging system (in vivo Ad-Luc). Simultaneously, I also applied the trans factor identification method through Transcription Factor Expression plasmid Library "TFEL" (TFEL scan) and found that KLF15 is important for the regulation of SREBP-1c expression in liver. It is known that KLF15 expression is upregulated during fasting, however, its regulatory mechanism has not yet been revealed. Therefore I investigated the regulatory mechanism of hepatic KLF15 gene expression by in vivo Ad-luc method and TFEL, and found new genomic region that important for KLF15 gene regulation during fasting and an important transcription factors binding to the region.
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