Production of Subtype Specific Cardiomyocytes from Human iPSCs Based on Metabolism
| Project/Area Number |
17H05067
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Keio University |
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Principal Investigator |
TOHYAMA Shugo 慶應義塾大学, 医学部(信濃町), 特任講師 (90528192)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2019: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2017: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Keywords | ヒトiPS細胞 / 代謝 / 心筋細胞 / 移植 / 創薬 / 幹細胞生物学 / 再生医療 / iPS細胞 / 再生医学 / 細胞移植 |
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| Outline of Final Research Achievements |
The purpose of this study is to develop an efficient method to produce subtype specific cardiomyocytes from human iPSCs via metabolic regulation for regenerative medicine and drug discovery. In this project, we identified optimal culture conditions for human iPSCs expansion (1). In addition, we also developed an efficient method to produce subtype specific cardiomyocytes from human iPSCs (2). Moreover, we evaluated metabolic profiles in subtype specific cardiomyocytes via multi-omics (3). These findings will eventually promote the clinical applications of regenerative medicine and drug discovery.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果の学術的意義は、代謝プロファイルを基盤にヒトiPS細胞の増殖能をコントロールするだけでなく、高純度心室筋細胞と非心室筋細胞を作り分ける技術を開発することで、表現型のばらつきを解消する点である。表現型のばらつきを解消することで、安全かつ有効な再生医療の実現化および創薬への応用が期待できるため、社会的意義も極めて高いと考えられる。
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Development of a transplant injection device for optimal distribution and retention of human induced pluripotent stem cell-derived cardiomyocytes2019
Author(s)
Tabei R, Kawaguchi S, Kanazawa H, Tohyama S, Hirano A, Handa N, Hishikawa S, Teratani T, Kunita S, Fukuda J, Mugishima Y, Suzuki T, Nakajima K, Seki T, Kishino Y, Okada M, Yamazaki M, Okamoto K, Shimizu H, Kobayashi E, Tabata Y, Fujita J, Fukuda K
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Journal Title
The Journal of heart and lung transplantation
Volume: 38
Issue: 2
Pages: 203-214
DOI
Related Report
Peer Reviewed
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[Journal Article] Selective elimination of undifferentiated human pluripotent stem cells using pluripotent state-specific immunogenic antigen Glypican-3.2019
Author(s)
Okada M, Tada Y, Seki T, Tohyama S, Fujita J, Suzuki T, Shimomura M, Ofuji K, Kishino Y, Nakajima K, Tanosaki S, Someya S, Kanazawa H, Senju S, Nakatsura T, Fukuda K.
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Journal Title
Biochem Biophys Res Commun.
Volume: 511
Issue: 3
Pages: 711-717
DOI
Related Report
Peer Reviewed
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[Journal Article] Efficient large-scale 2D culture system for human induced pluripotent stem cells and differentiated cardiomyocytes.2017
Author(s)
Toyama S, Fujita J*, Fujita C, Yamaguchi M, Kanaami S, Ohno R, Sakamoto K, Kodama M, Kurokawa J, Kanazawa H, Seki T, Kishino Y, Okada M, Nakajima K, Tanosaki S, Someya S, Hirano A, Kawaguchi S, Kobayashi E, Fukuda K
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Journal Title
Stem Cell Rep
Volume: 9
Issue: 5
Pages: 1406-1414
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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