Herpesvirus-mediated regulatory mechanism of cellular phosphorylation events

• Project/Area Number: 17H05069
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: The University of Tokyo
• Principal Investigator: Kato Akihisa 東京大学, 医科学研究所, 准教授 (40581187)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000); Fiscal Year 2019: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2017: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
• Keywords: 単純ヘルペスウイルス / mTOR / TLR3 / MAPK / ERK / ヘルペス脳炎 / 細胞間伝播 / HSV / Us3 / 宿主細胞基質 / キノーム / ドラックプロファイリング / プロテインキナーゼ / mTORC2 / HSV脳炎 / HSV-1 / ドラッグリプロファイリング / ヘルペス性脳炎 / キノーム解析 / リン酸化

Outline of Final Research Achievements

In this study, we investigated whether viral replication and/or virulence are regulated by herpesvirus-induced cellular phosphorylation events. We found that (i) the TLR3/mTOR axis contributed to the innate immune response against herpes simplex virus in the central nervous system of mice and (ii) the MAPK/ERK signaling pathway was involved in the cell-to-cell spread of representative members from all herpesvirus subfamilies. Collectively, these results reveal an important role of cellular phosphorylation in the life cycle of herpesviruses, thereby opening up possibilities for new therapeutic strategies against these viral pathogens.

Academic Significance and Societal Importance of the Research Achievements

周知の通り、宿主キナーゼは極めて有望な創薬標的分子であることから、本研究成果は、ヘルペスウイルス感染症の克服に目掛けた重要な基礎的知見となることが期待される。また、数多くのウイルスが効率的な増殖に宿主キナーゼを利用することから、本研究で確立した研究手法や得られた知見は、他のウイルス研究にもフィードバックが可能であると考えられる。

Research Products

• [Journal Article] Role of the DNA Binding Activity of Herpes Simplex Virus 1 VP22 in Evading AIM2-Dependent Inflammasome Activation Induced by the Virus (2020)
  • Author(s): Maruzuru Yuhei、Koyanagi Naoto、Kato Akihisa、Kawaguchi Yasushi
  • Journal Title: Journal of Virology Volume: 95 Issue: 5
  • DOI: 10.1128/jvi.02172-20
  • Peer Reviewed / Open Access
• [Journal Article] Prohibitin-1 Contributes to Cell-to-Cell Transmission of Herpes Simplex Virus 1 via the MAPK/ERK Signaling Pathway (2020)
  • Author(s): Watanabe Mizuki、Arii Jun、Takeshima Kosuke、Fukui Ayano、Shimojima Masayuki、Kozuka-Hata Hiroko、Oyama Masaaki、Minamitani Takeharu、Yasui Teruhito、Kubota Yuji、Takekawa Mutsuhiro、Kosugi Isao、Maruzuru Yuhei、Koyanagi Naoto、Kato Akihisa、Mori Yasuko、Kawaguchi Yasushi
  • Journal Title: Journal of Virology Volume: 95 Issue: 3
  • DOI: 10.1128/jvi.01413-20
  • Peer Reviewed / Open Access
• [Journal Article] Identification of a herpes simplex virus 1 gene encoding neurovirulence factor by chemical proteomics (2020)
  • Author(s): Kato Akihisa、Adachi Shungo、Kawano Shuichi、Takeshima Kousuke、Watanabe Mizuki、Kitazume Shinobu、Sato Ryota、Kusano Hideo、Koyanagi Naoto、Maruzuru Yuhei、Arii Jun、Hatta Tomohisa、Natsume Tohru、Kawaguchi Yasushi
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 4894-4894
  • DOI: 10.1038/s41467-020-18718-9
  • Peer Reviewed / Open Access
• [Journal Article] Role of Phosphatidylethanolamine Biosynthesis in Herpes Simplex Virus 1-Infected Cells in Progeny Virus Morphogenesis in the Cytoplasm and in Viral Pathogenicity in Vivo (2020)
  • Author(s): Arii Jun、Fukui Ayano、Shimanaka Yuta、Kono Nozomu、Arai Hiroyuki、Maruzuru Yuhei、Koyanagi Naoto、Kato Akihisa、Mori Yasuko、Kawaguchi Yasushi
  • Journal Title: Journal of Virology Volume: 94 Issue: 24
  • DOI: 10.1128/jvi.01572-20
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Phosphoregulation of a Conserved Herpesvirus Tegument Protein by a Virally Encoded Protein Kinase in Viral Pathogenicity and Potential Linkage between Its Evolution and Viral Phylogeny (2020)
  • Author(s): Shibazaki Misato、Kato Akihisa、Takeshima Kosuke、Ito Jumpei、Suganami Mai、Koyanagi Naoto、Maruzuru Yuhei、Sato Kei、Kawaguchi Yasushi
  • Journal Title: Journal of Virology Volume: 94 Issue: 18 Pages: 1-4
  • DOI: 10.1128/jvi.01055-20
  • Peer Reviewed / Open Access
• [Journal Article] ESCRT-III controls nuclear envelope deformation induced by progerin (2020)
  • Author(s): Arii Jun、Maeda Fumio、Maruzuru Yuhei、Koyanagi Naoto、Kato Akihisa、Mori Yasuko、Kawaguchi Yasushi
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 18877-18877
  • DOI: 10.1038/s41598-020-75852-6
  • Peer Reviewed / Open Access
• [Journal Article] Long noncoding RNA U90926 is crucial for herpes simplex virus type 1 proliferation in murine retinal photoreceptor cells (2020)
  • Author(s): Shirahama Shintaro、Onoguchi-Mizutani Rena、Kawata Kentaro、Taniue Kenzui、Miki Atsuko、Kato Akihisa、Kawaguchi Yasushi、Tanaka Rie、Kaburaki Toshikatsu、Kawashima Hidetoshi、Urade Yoshihiro、Aihara Makoto、Akimitsu Nobuyoshi
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 19406-19406
  • DOI: 10.1038/s41598-020-76450-2
  • Peer Reviewed / Open Access
• [Journal Article] How does spaceflight affect acquired immune system? (2020)
  • Author(s): T. Akiyama, K. Horie, E. Hinoi, M. Hiraiwa, A. Kato, Y. Maekawa, A. Takahashi, S. Furukawa
  • Journal Title: NPJ Microgravity. Volume: 0 Issue: 1 Pages: 1-7
  • DOI: 10.1038/s41526-020-0104-1
  • Peer Reviewed / Open Access
• [Journal Article] Identification of the Capsid Binding Site in the Herpes Simplex Virus 1 Nuclear Egress Complex and Its Role in Viral Primary Envelopment and Replication. (2019)
  • Author(s): Takeshima K, Arii J, Maruzuru Y, Koyanagi N, Kato A, Kawaguchi Y.
  • Journal Title: J Virol. Volume: 93 Issue: 21
  • DOI: 10.1128/jvi.01290-19
  • Peer Reviewed / Open Access
• [Journal Article] Roles of the Interhexamer Contact Site for Hexagonal Lattice Formation of the Herpes Simplex Virus 1 Nuclear Egress Complex in Viral Primary Envelopment and Replication. (2019)
  • Author(s): Arii J, Takeshima K, Maruzuru Y, Koyanagi N, Kato A, Kawaguchi Y.
  • Journal Title: J Virol. Volume: 93 Issue: 14
  • DOI: 10.1128/jvi.00498-19
  • Peer Reviewed / Open Access
• [Journal Article] Combating herpesvirus encephalitis by potentiating a TLR3-mTORC2 axis. (2018)
  • Author(s): Sato R, Kato A, Chimura T, Saitoh SI, Shibata T, Murakami Y, Fukui R, Liu K, Zhang Y, Arii J, Sun-Wada GH, Wada Y, Ikenoue T, Barber GN, Manabe T, Kawaguchi Y, Miyake K.
  • Journal Title: Nat Immunol. Volume: 19 Issue: 10 Pages: 1071-1082
  • DOI: 10.1038/s41590-018-0203-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity. (2018)
  • Author(s): Arii J, Watanabe M, Maeda F, Tokai-Nishizumi N, Chihara T, Miura M, Maruzuru Y, Koyanagi N, Kato A, Kawaguchi Y.
  • Journal Title: Nat Commun. Volume: 9 Issue: 1 Pages: 3379-3379
  • DOI: 10.1038/s41467-018-05889-9
  • Peer Reviewed / Open Access
• [Journal Article] Roles of the Phosphorylation of Herpes Simplex Virus 1 UL51 at a Specific Site in Viral Replication and Pathogenicity. (2018)
  • Author(s): Kato A, Oda S, Watanabe M, Oyama M, Kozuka-Hata H, Koyanagi N, Maruzuru Y, Arii J, Kawaguchi Y.
  • Journal Title: J Virol. Volume: 92 Issue: 18
  • DOI: 10.1128/jvi.01035-18
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of Herpes Simplex Virus 2 Protein Kinase UL13 by Phosphorylation and Its Role in Viral Pathogenesis. (2018)
  • Author(s): Koyanagi N, Kato A, Takeshima K, Maruzuru Y, Kozuka-Hata H, Oyama M, Arii J, Kawaguchi Y.
  • Journal Title: J Virol. Volume: 92 Issue: 17
  • DOI: 10.1128/jvi.00807-18
  • Peer Reviewed / Open Access
• [Journal Article] Herpes Simplex Virus 1 VP22 Inhibits AIM2-Dependent Inflammasome Activation to Enable Efficient Viral Replication. (2018)
  • Author(s): Maruzuru Y, Ichinohe T, Sato R, Miyake K, Okano T, Suzuki T, Koshiba T, Koyanagi N, Tsuda S, Watanabe M, Arii J, Kato A, Kawaguchi Y.
  • Journal Title: Cell Host Microbe. Volume: 23 Issue: 2 Pages: 254-265
  • DOI: 10.1016/j.chom.2017.12.014
  • Peer Reviewed / Open Access
• [Journal Article] Us3 protein kinase encoded by HSV: the precise function and mechanism on viral life cycle. (2018)
  • Author(s): A. Kato and Y. Kawaguchi.
  • Journal Title: Advances in Experimental Medicine and Biology Volume: 1045 Pages: 45-62
  • DOI: 10.1007/978-981-10-7230-7_3
  • ISBN: 9789811072291, 9789811072307
  • Peer Reviewed
• [Journal Article] Herpes simplex virus-1 evasion of CD8+ T cell accumulation contributes to viral encephalitis. (2017)
  • Author(s): Koyanagi N, Imai T, Shindo K, Sato A, Fujii W, Ichinohe T, Takemura N, Kakuta S, Uematsu S, Kiyono H, Maruzuru Y, Arii J, Kato A, Kawaguchi Y.
  • Journal Title: J Clin Invest. Volume: 127 Issue: 10 Pages: 3784-3795
  • DOI: 10.1172/jci92931
  • Peer Reviewed / Open Access
• [Journal Article] Herpes Simplex Virus 1 Small Capsomere-Interacting Protein VP26 Regulates Nucleocapsid Maturation. (2017)
  • Author(s): Kobayashi R, Kato A, Sagara H, Watanabe M, Maruzuru Y, Koyanagi N, Arii J, Kawaguchi Y.
  • Journal Title: J Virol. Volume: 91 Issue: 18
  • DOI: 10.1128/jvi.01068-17
  • Peer Reviewed / Open Access
• [Journal Article] Herpes Simplex Virus 1 UL34 Protein Regulates the Global Architecture of the Endoplasmic Reticulum in Infected Cells. (2017)
  • Author(s): F. Maeda, J. Arii, Y. Hirohata, Y. Maruzuru, N. Koyanagi, A. Kato and Y. Kawaguchi.
  • Journal Title: J. Virol. Volume: 印刷中 Issue: 12
  • DOI: 10.1128/jvi.00271-17
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 宇宙開発の基盤となる潜伏感染症の横断的解析 (2020)
  • Author(s): 加藤哲久
  • Organizer: 新学術領域「宇宙に生きる」オンライン全体会議
• [Presentation] 核膜間交流ゾーンにおける分子基盤と生物学的意義の解明 (2020)
  • Author(s): 加藤哲久
  • Organizer: 新学術領域「オルガネラ ・ゾーン」班会議
• [Presentation] UL7 リン酸化制御機構の獲得とαヘルペスウイルス亜科の進化 (2019)
  • Author(s): 柴崎美里, 加藤哲久, 伊東潤平, 菅波麻衣, 竹島功高, 丸鶴雄平, 小栁直人, 有井潤, 佐藤佳, 川口寧
  • Organizer: 第33回ヘルペスウイルス研究会
• [Presentation] HSV UL13 プロテインキナーゼの新規キナーゼ活性制御機構の解明 (2019)
  • Author(s): 小栁直人, 加藤哲久, 丸鶴雄平, 有井潤, 川口寧
  • Organizer: 第33回ヘルペスウイルス研究会
• [Presentation] HSV-1 Nuclear Egress Complex とヌクレオカプシドとの結合は, Primary Envelopment に寄与する (2019)
  • Author(s): 竹島功高, 有井潤, 丸鶴雄平, 小栁直人, 加藤哲久, 川口寧
  • Organizer: 第33回ヘルペスウイルス研究会
• [Presentation] HSV-1 Us3 protein kinaseの基質特異性を司るリン酸化制御機構の解明 (2019)
  • Author(s): 木村南美, 加藤哲久，五十嵐学, 丸鶴雄平，小栁直人，有井潤，川口寧
  • Organizer: 第33回ヘルペスウイルス研究会
• [Presentation] 新規HSV-1 遺伝子の機能解析 (2019)
  • Author(s): 加藤哲久，竹島功高, 丸鶴雄平，小栁直人，有井潤，川口寧, 足達俊吾, 八田知久, 夏目徹
  • Organizer: 第33回ヘルペスウイルス研究会
• [Presentation] Identification of the Capsid Binding Site in the Herpes Simplex Virus 1 Nuclear Egress Complex and Its Role in Viral Primary Envelopment and Replication (2019)
  • Author(s): Kosuke Takeshima, Jun Arii, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi
  • Organizer: 第67回日本ウイルス学会学術集会
• [Presentation] Regulatory mechanism of the substrate specificity of Us3 protein kinase encoded by herpes simplex virus-1 (2019)
  • Author(s): Akihisa Kato, Nami Kimura, Naoto Koyanagi, Yuhei Maruzuru, Jun Arii, Manabu Igarashi, Yasushi Kawaguchi
  • Organizer: 第67回日本ウイルス学会学術集会
• [Presentation] The roles of glycerophospholipids in HSV-1 replication (2019)
  • Author(s): Jun Arii, Ayano Fukui, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi
  • Organizer: 第67回日本ウイルス学会学術集会
• [Presentation] The role of Us3 mediated UL7 phosphorylation during the evolution of α-herpesviruses (2019)
  • Author(s): Misato Shibazaki, Akihisa Kato, Jumpei Ito, Mai Suganami, Kosuke Takeshima, Yuhei Maruzuru, Naoto Koyanagi, Jun Arii, Kei Sato, Yasushi Kawaguchi
  • Organizer: 第67回日本ウイルス学会学術集会
• [Presentation] Evolutionary analysis of UL7 phosphorylation events encoded by α-herpesviruses (2019)
  • Author(s): Misato Shibazaki, Akihisa Kato, Jumpei Ito, Mai Suganami, Kosuke Takeshima, Yuhei Maruzuru, Naoto Koyanagi, Jun Arii, Kei Sato, and Yasushi Kawaguchi
  • Organizer: 第18回あわじ感染と免疫国際フォーラム
  • Int'l Joint Research
• [Presentation] Identification of a cellular protein that interacts with the HSV-1 UL31/UL34 complex and regulates HSV-1 de-envelopment (2019)
  • Author(s): Fumio Maeda, Jun Arii, Misato Shibazaki, Keiko Shindo, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato and Yasushi Kawaguchi
  • Organizer: 第18回あわじ感染と免疫国際フォーラム
  • Int'l Joint Research
• [Presentation] Identification of the Capsid Binding Site in the Herpes Simplex Virus 1 Nuclear Egress Complex and Its Role in Viral Primary Envelopment and Replication (2019)
  • Author(s): Kosuke Takeshima, Jun Arii, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, and Yasushi Kawaguchi
  • Organizer: 第18回あわじ感染と免疫国際フォーラム
  • Int'l Joint Research
• [Presentation] Roles of the Inter-Hexamer Contact Site for Hexagonal Lattice Formation of Herpes Simplex Virus 1 Nuclear Egress Complex in Viral Primary Envelopment and Replication (2019)
  • Author(s): Jun Arii, Kosuke Takeshima, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi
  • Organizer: 第18回あわじ感染と免疫国際フォーラム
  • Int'l Joint Research
• [Presentation] HSV-1 UL-31/UL-34六量体間結合は、核膜間のウイルス粒子形成とウイルス増殖に貢献する (2018)
  • Author(s): 有井潤, 竹島功高, 丸鶴雄平, 小栁直人, 加藤哲久, 川口寧
  • Organizer: 第32回ヘルペスウイルス研究会
• [Presentation] Primary EnvelopmentにおけるHSV-1 UL31 α-helix9の役割 (2018)
  • Author(s): 竹島功高, 有井潤, 丸鶴雄平, 小栁直人, 加藤哲久, 川口寧
  • Organizer: 第32回ヘルペスウイルス研究会
• [Presentation] αヘルペスウイルスの進化とUs3リン酸化部位の獲得 (2018)
  • Author(s): 柴﨑美里, 加藤哲久, 竹島功高, 丸鶴雄平, 小栁直人, 有井潤, 川口寧
  • Organizer: 第32回ヘルペスウイルス研究会
• [Presentation] 新規HSV-1遺伝子の同定 (2018)
  • Author(s): 加藤哲久, 竹島功高, 丸鶴雄平, 小栁直人, 有井潤, 川口寧, 川野秀一, 足達俊吾, 八田知久, 夏目徹
  • Organizer: 第32回ヘルペスウイルス研究会
• [Presentation] A single amino acid substitution in herpes simplex virus 1 UL31 at an inter-hexamer contact site abrogates primary envelopment and viral growth (2018)
  • Author(s): Jun Arii, Kosuke Takeshima, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi
  • Organizer: 43rd Annual International Herpesvirus Worksho
  • Int'l Joint Research
• [Presentation] A HSV-2 specific phosphorylation event mediated by Us3 kinase is involved in viral pathogenicity in mice following vaginal infection (2018)
  • Author(s): Misato Shibazaki, Akihisa Kato, Naoto Koyanagi, Yuhei Maruzuru, Jun Arii, Yasushi Kawaguchi
  • Organizer: 43rd Annual International Herpesvirus Workshop
  • Int'l Joint Research
• [Presentation] Role of amino acid residues in the α-helix 9 of HSV-1 UL31 in its interaction with capsid proteins and nuclear egress (2018)
  • Author(s): Kosuke Takeshima, Jun Arii, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi
  • Organizer: 43rd Annual International Herpesvirus Workshop
  • Int'l Joint Research
• [Presentation] Roles of Us8A and Its Phosphorylation Mediated by Us3 in HSV-1 Pathogenesis (2018)
  • Author(s): Akihisa Kato
  • Organizer: 第121回日本小児科学会学術集会
• [Presentation] 単純ヘルペスウイルス（HSV）の細胞間伝播を促進する宿主細胞膜タンパク質の同定 (2017)
  • Author(s): 渡邉瑞季, 有井潤, 下島昌幸, 加藤哲久, 川口寧
  • Organizer: 第14回ウイルス学キャンプ in 湯河原
• [Presentation] HSV-1 UL51 リン酸化制御機構と細胞種依存的な新規機能の解明 (2017)
  • Author(s): 尾田真也, 加藤哲久, 丸鶴雄平, 小栁直人, 有井潤, 川口寧
  • Organizer: 第31回ヘルペスウイルス研究会
• [Presentation] HSV-2のウイルス増殖および病原性はUL13プロテインキナーゼのリン酸化によって制御される (2017)
  • Author(s): 小栁直人, 加藤哲久, 丸鶴雄平, 有井潤, 川口寧
  • Organizer: 第31回ヘルペスウイルス研究会
• [Presentation] ESCRT-IIIは小胞媒介性核外輸送における膜切断を仲介し、核内膜の恒常性維持に寄与する (2017)
  • Author(s): 有井潤, 渡邉瑞季, 前田史雄, 加藤哲久, 川口寧
  • Organizer: 第31回ヘルペスウイルス研究会
• [Presentation] Inhibition of AIM2 inflammasome activation by an HSV-1 virion component enables efficient viral replication in vivo (2017)
  • Author(s): Yuhei Maruzuru, Takeshi Ichinohe, Naoto Koyanagi, Jun Arii, Akihisa Kato and Yasushi Kawaguchi
  • Organizer: 第31回ヘルペスウイルス研究会
• [Presentation] 単純ヘルペスウイルス1型UL34は小胞体の形態制御を行い、ヌクレオカプシドの核出芽輸送制御因子の核膜への集積に関与する (2017)
  • Author(s): 前田史雄, 廣畑吉崇, 有井潤, 加藤哲久, 川口寧
  • Organizer: 第31回ヘルペスウイルス研究会
• [Presentation] 単純ヘルペスウイルス(HSV)の細胞間感染を促進する宿主タンパク質の同定 (2017)
  • Author(s): 渡邉瑞季, 有井潤, 下島昌幸, 加藤哲久, 川口寧
  • Organizer: 第31回ヘルペスウイルス研究会
• [Presentation] ESCRT-III mediates vesicle-mediated nuclear export to regulate nuclear egress of HSV-1 capsids and integrity of the inner nuclear membrane (2017)
  • Author(s): Jun Arii, Mizuki Watanabe, Fumio Maeda, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi
  • Organizer: 第65回日本ウイルス学会学術集会
• [Presentation] Cell type-specific regulation of the nuclear egress of herpes simplex virus type1 by phosphorylation of UL51 Ser-184 (2017)
  • Author(s): Shinya Oda, Akihisa Kato, Yuhei Maruzuru, Naoto Koyanagi, Jun Arii, Yasushi Kawaguchi
  • Organizer: 第65回日本ウイルス学会学術集会
• [Presentation] Evasion of AIM2-Dependent Inflamasome Activation by Herpes Simplex Virus 1 Virion Protein Enables Efficient Viral Replication In Vivo (2017)
  • Author(s): Yuhei Maruzuru, Takeshi Ichinohe, Naoto Koyanagi, Zhuoming Liu, Tomoko Ando, Jun Arii, Akihisa Kato, Yasushi Kawaguchi
  • Organizer: 第65回日本ウイルス学会学術集会
• [Presentation] A host cell membrane protein interacts with HSV-1 gE and promotes viral cell-to-cell spread (2017)
  • Author(s): Mizuki Watanabe, Jun Arii, Masayuki Shimojima, Akihisa Kato, Yasushi Kawaguchi
  • Organizer: 第65回日本ウイルス学会学術集会
• [Presentation] Function of the herpes simplex virus 2 protein kinase UL13 in vitro and in vivo is regulated by phosphorylation (2017)
  • Author(s): Naoto Koyanagi, Akihisa Kato, Yuhei Maruzuru, Jun Arii, Yasushi Kawaguchi
  • Organizer: 第65回日本ウイルス学会学術集会
• [Presentation] Inhibition of AIM2 inflammasome activation by an HSV-1 virion component enables efficient viral replication in vivo (2017)
  • Author(s): Yuhei Maruzuru, Takeshi Ichinohe, Naoto Koyanagi, Jun Arii, Akihisa Kato and Yasushi Kawaguchi
  • Organizer: 42nd Annual International Herpesvirus Workshop
  • Int'l Joint Research
• [Presentation] Identification of a host cell membrane protein that interacts with HSV-1 gE and promotes viral cell-to-cell spread (2017)
  • Author(s): Mizuki Watanabe, Jun Arii, Masayuki Shimojima, Naoto Koyanagi, Yuhei Maruzuru, Akihisa Kato and Yasushi Kawaguchi
  • Organizer: 42nd Annual International Herpesvirus Workshop
  • Int'l Joint Research
• [Presentation] Herpes simplex virus type 1 UL51 Protein Regulates Virion Morphogenesis in the Nucleus in a Cell type and Phosphorylation-Dependent Manner (2017)
  • Author(s): Shinya Oda, Akihisa Kato, Naoto Koyanagi, Yuhei Maruzuru, Jun Arii and Yasushi Kawaguchi
  • Organizer: 42nd Annual International Herpesvirus Workshop
  • Int'l Joint Research
• [Presentation] ESCRT-III mediates vesicle-mediated nuclear export and regulates the integrity of the inner nuclear membrane (2017)
  • Author(s): Jun Arii, Mizuki Watanabe, Fumio Maeda, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato and Yasushi Kawaguchi
  • Organizer: 42nd Annual International Herpesvirus Workshop
  • Int'l Joint Research
• [Presentation] Regulation of herpes simplex virus-2 UL13 protein kinase by phosphorylation is important for viral replication, pathogenic manifestations, and virulence in vivo (2017)
  • Author(s): Naoto Koyanagi, Akihisa Kato, Yuhei Maruzuru, Jun Arii and Yasushi Kawaguchi
  • Organizer: 42nd Annual International Herpesvirus Workshop
  • Int'l Joint Research
• [Presentation] Herpes simplex 1 UL34 Protein Regurates the Global Architecture of the Endoplasmic Reticulum in Infected Cells (2017)
  • Author(s): Fumio Maeda, Jun Arii, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato and Yasushi Kawaguchi
  • Organizer: 42nd Annual International Herpesvirus Workshop
  • Int'l Joint Research
• [Presentation] Impact of phosphorylation of two viral proteins on the herpes simplex virus-life cycle in vitro and in vivo (2017)
  • Author(s): Akihisa Kato, Yasushi Kawaguchi
  • Organizer: 9th Inhibitors of Protein Kinases Conference (IPK2017)
  • Int'l Joint Research
• [Remarks]
  • URL: https://www.ims.u-tokyo.ac.jp/Kawaguchi-lab/KawaguchiLabTop.html
• [Remarks] 東京大学医科学研究所 感染・免疫部門 ウイルス病態制御分野ホームページ
  • URL: https://www.ims.u-tokyo.ac.jp/Kawaguchi-lab/KawaguchiLabTop.html
• [Remarks] 東京大学医科学研究所 感染・免疫部門 ウイルス病態制御分野ホームページ
  • URL: http://www.ims.u-tokyo.ac.jp/Kawaguchi-lab/KawaguchiLabTop.html