| Budget Amount *help |
¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000)
Fiscal Year 2019: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2017: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
|
|---|
| Outline of Final Research Achievements |
Positive strand RNA viruses initiate genome replication within specialized membrane vesicles, designated "replication factory", in infected cells. Such organelles are typically induced by engagement of viral proteins and associated with morphological changes in the structure of host internal membranes. Whereas the process involved in formation of the replication factory must involve alterations in host lipid metabolism, the mechanistic details remain poorly characterized. Through investigations by means of microarray analysis, comprehensive lipidomics analysis and fatty acid profiling, this study has revealed that RNA viruses modulate cellular lipid profiles via modulation of cellular phospholipase activity that cleaves fatty acid tails from membrane phospholipids as well as fatty acid desaturases. Importantly, analyses of fatty acid metabolic pathways that catalyze fatty acid cleavage or desaturation identified host targets that could lead to development of virus-specific antivirals.
|
