| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2017: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
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| Outline of Final Research Achievements |
To elucidate the transcriptional regulation of the ABO gene, we generated hematopoietic or epithelial cell-specific enhancer region deletion cell lines using the CRISPR/Cas system. These cells were used for RNA sequencing and real-time PCR to determine which genes were down-regulated compared to the wild type. The epithelial cell-specific enhancer region deletion cell line using KATOIII gastric cancer cells showed decreased expression of the ABO gene and the OBP2B gene, which is downstream of the ABO gene. These results indicate that the ABO gene is transcriptionally regulated, including sharing of enhancers and regulation of gene expression via higher order structure of chromosomes.
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