| Budget Amount *help |
¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2019: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2017: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
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| Outline of Final Research Achievements |
This study used two approaches to generate functional testicular organoid. First, the architecture of seminiferous tubule was imitated by the sheet device in which germ cells were incubated on Sertoli cell sheet. By the device, Sertoli cell sheet was maintained over 3months and germ cell were divided into the early meiotic stages. Second, testicular cells were incubated by the self-organization technique. Germ cells were arranged in a chain at the outer area of organoids and were divided into the early meiotic stages.
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