Understanding of Runx2-mediated hierarichical gene regulatory network in bone formation and application of the network for bone regeneration
| Project/Area Number |
17H05106
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Hojo Hironori 東京大学, 大学院医学系研究科(医学部), 准教授 (80788422)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥24,440,000 (Direct Cost: ¥18,800,000、Indirect Cost: ¥5,640,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2017: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Keywords | 転写制御 / 骨発生 / Runx2 / エピゲノム / 転写因子 / 発生・分化 / 発現制御 / バイオインフォマティクス / 骨形成 |
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| Outline of Final Research Achievements |
Runx2 is an essential transcription factor for both osteoblast specification and chondrocyte hypertrophy. In this study, we performed integrative analysis of Runx2-DNA binding profile and cell-type distinct epigenetic landscape profile in mouse neonatal osteoblasts and chondrocytes. We identified cell-type specific putative enhancers targeted by Runx2 in osteoblasts and chondrocytes, and confirmed a biological function of these. Through this study, we identified a Runx2-mediated gene regulatory program in skeletal development.
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| Academic Significance and Societal Importance of the Research Achievements |
転写因子Runx2は骨を形成する骨芽細胞の分化決定・成熟化を促進する作用と、軟骨組織に存在する軟骨細胞の分化・肥大化を進める作用を有しており、これらのメカニズム解明は、骨格組織の再生医療法の確立や疾患の分子病態の理解に必須である。本研究では、Runx2の骨芽細胞と軟骨細胞に対する作用メカニズムの一端を、ゲノムワイドな転写制御機構の観点から明らかにし、骨格再生に向けた基礎的知見を取得した。
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Generation of orthotopically functional salivary gland from embryonic stem cells2018
Author(s)
Tanaka Junichi、Ogawa Miho、Hojo Hironori、Kawashima Yusuke、Mabuchi Yo、Hata Kenji、Nakamura Shiro、Yasuhara Rika、Takamatsu Koki、Irie Tarou、Fukada Toshiyuki、Sakai Takayoshi、Inoue Tomio、Nishimura Riko、Ohara Osamu、Saito Ichiro、Ohba Shinsuke、Tsuji Takashi、Mishima Kenji
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Journal Title
Nature Communications
Volume: 9
Issue: 1
Pages: 4216-4216
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] De novo missense mutation in SP7 in a patient with cranial hyperostosis, long bone fragility, and increased osteoblast number.2019
Author(s)
Lui J, Raimann A, Dong L, Hojo H, Roschger P, Fratzl-Zelman N, Jee YH, Haeusler G, Baron J
Organizer
2019 Annual Meeting of the American Society for Bone and Mineral Research
Related Report
Int'l Joint Research
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