In situ functional analyses of membrane proteins by NMR

• Project/Area Number: 17H06097
• Research Category: Grant-in-Aid for Specially Promoted Research
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Institute of Physical and Chemical Research (2019-2022); The University of Tokyo (2017-2018)
• Principal Investigator: Shimada Ichio 国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (70196476)
• Co-Investigator(Kenkyū-buntansha): 上田 卓見 東京大学, 大学院薬学系研究科(薬学部), 准教授 (20451859) ; 竹内 恒 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ長 (20581284) ; 西田 紀貴 千葉大学, 大学院薬学研究院, 教授 (50456183)
• Project Period (FY): 2017-04-25 – 2022-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥460,330,000 (Direct Cost: ¥354,100,000、Indirect Cost: ¥106,230,000); Fiscal Year 2021: ¥87,360,000 (Direct Cost: ¥67,200,000、Indirect Cost: ¥20,160,000); Fiscal Year 2020: ¥94,510,000 (Direct Cost: ¥72,700,000、Indirect Cost: ¥21,810,000); Fiscal Year 2019: ¥87,360,000 (Direct Cost: ¥67,200,000、Indirect Cost: ¥20,160,000); Fiscal Year 2018: ¥105,300,000 (Direct Cost: ¥81,000,000、Indirect Cost: ¥24,300,000); Fiscal Year 2017: ¥85,800,000 (Direct Cost: ¥66,000,000、Indirect Cost: ¥19,800,000)
• Keywords: 核磁気共鳴法 / Gタンパク質共役型受容体 / イオンチャネル / 多剤耐性トランスポーター / 動的構造平衡 / NMR / 膜タンパク質 / 動的構造 / GPCR / 動的立体構造

Outline of Final Research Achievements

In this study, we developed various novel NMR methods to analyze the dynamic structures of high molecular weight proteins under physiological in situ conditions and applied these methods to biologically important membrane proteins such as G protein-coupled receptors (GPCRs), ion channels, and multidrug resistance transporters. As a result, we successfully elucidated the regulatory mechanisms underlying the function of these membrane proteins based on dynamic structural information.

Academic Significance and Societal Importance of the Research Achievements

本研究では、生命現象で重要な役割を果たす膜タンパク質が、構造平衡を介して機能を発揮することを示した。創薬標的タンパク質の動的構造と機能を直接結ぶ我々の研究は、X線結晶解析やクライオ電子顕微鏡、AlphaFold2によってタンパク質の3次元構造決定を目指す研究が隆盛する現在において、タンパク質の機能解明にはダイナミクス、特に時間軸を考慮する必要性を示している点で学術的意義が高い。また、動的構造を制御するような新たな創薬点を提示する点において、社会的な意義が高い。

Assessment Rating

Verification Result (Rating)

A+

Research Products

• [Int'l Joint Research] トロント大学(カナダ)
• [Int'l Joint Research] スクリプス研究所(米国)
• [Int'l Joint Research] ヘルシンキ大学(フィンランド)
• [Int'l Joint Research] 復旦大学 生命科学学院(中国)
• [Int'l Joint Research] トロント大学(カナダ)
• [Journal Article] Activation mechanism of the μ-opioid receptor by an allosteric modulator. (2022)
  • Author(s): Kaneko S, Imai S, Asao N, Kofuku Y, Ueda T, Shimada I.
  • Journal Title: Proc. Natl. Acad. Sci. U. S. A. Volume: 119 Issue: 16
  • DOI: 10.1073/pnas.2121918119
  • Peer Reviewed / Open Access
• [Journal Article] Function-related dynamics of GPCRs (2022)
  • Author(s): Ueda Takumi、Imai Shunsuke、Shimada Ichio
  • Journal Title: Journal of Magnetic Resonance Volume: 336 Pages: 107164-107164
  • DOI: 10.1016/j.jmr.2022.107164
  • Peer Reviewed
• [Journal Article] Biphasic activation of β-arrestin 1 upon interaction with a GPCR revealed by methyl-TROSY NMR (2021)
  • Author(s): Shiraishi Yutaro、Kofuku Yutaka、Ueda Takumi、Pandey Shubhi、Dwivedi-Agnihotri Hemlata、Shukla Arun K.、Shimada Ichio
  • Journal Title: Nature Communications Volume: 12 Issue: 1 Pages: 7158-7158
  • DOI: 10.1038/s41467-021-27482-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Function-Related Dynamics in Multi-Spanning Helical Membrane Proteins Revealed by Solution NMR (2021)
  • Author(s): Takeuchi Koh、Kofuku Yutaka、Imai Shunsuke、Ueda Takumi、Tokunaga Yuji、Toyama Yuki、Shiraishi Yutaro、Shimada Ichio
  • Journal Title: Membranes Volume: 11 Issue: 8 Pages: 604-604
  • DOI: 10.3390/membranes11080604
  • Peer Reviewed / Open Access
• [Journal Article] Conformational Plasticity of Cyclic Ras‐Inhibitor Peptides Defines Cell Permeabilization Activity (2021)
  • Author(s): Takeuchi Koh、Misaki Imai、Tokunaga Yuji、Fujisaki Miwa、Kamoshida Hajime、Takizawa Takeshi、Hanzawa Hiroyuki、Shimada Ichio
  • Journal Title: Angewandte Chemie International Edition Volume: 60 Issue: 12 Pages: 6567-6572
  • DOI: 10.1002/anie.202016647
  • Peer Reviewed
• [Journal Article] NMR法を用いた中分子環状ペプチドの立体構造解析と細胞内移行活性の理 (2021)
  • Author(s): 竹内恒、嶋田一夫
  • Journal Title: Pharm Stage Volume: 21 Pages: 8-8
• [Journal Article] NMRを用いた動的構造解析への取り組みと創薬への展開 (2021)
  • Author(s): 竹内恒
  • Journal Title: 日本核磁気共鳴学会機関紙 Volume: 11 Pages: 27-34
• [Journal Article] バイオリアクター型in-cell NMR法の開発とRasの活性状態の観測への応用 (2021)
  • Author(s): 西田紀貴
  • Journal Title: 生化学 Volume: 93 Pages: 819-823
  • NAID: 40022783951
• [Journal Article] 分子夾雑系が変調するタンパク質の構造と機能 (2021)
  • Author(s): 趙慶慈、嶋田一夫、西田紀貴
  • Journal Title: 月刊「細胞」 Volume: 53 Pages: 352-355
• [Journal Article] Mechanism of hERG inhibition by gating-modifier toxin, APETx1, deduced by functional characterization (2021)
  • Author(s): Matsumura Kazuki、Shimomura Takushi、Kubo Yoshihiro、Oka Takayuki、Kobayashi Naohiro、Imai Shunsuke、Yanase Naomi、Akimoto Madoka、Fukuda Masahiro、Yokogawa Mariko、Ikeda Kazuyoshi、Kurita Jun-ichi、Nishimura Yoshifumi、Shimada Ichio、Osawa Masanori
  • Journal Title: BMC Molecular and Cell Biology Volume: 22 Issue: 1 Pages: 1-16
  • DOI: 10.1186/s12860-020-00337-3
  • Peer Reviewed / Open Access
• [Journal Article] Structural Fingerprints of an Intact Monoclonal Antibody Acquired under Formulated Storage Conditions via 15N Direct Detection Nuclear Magnetic Resonance (2020)
  • Author(s): Tokunaga Yuji、Takeuchi Koh、Okude Junya、Ori Kazutomo、Torizawa Takuya、Shimada Ichio
  • Journal Title: Journal of Medicinal Chemistry Volume: - Issue: 10 Pages: 5360-5366
  • DOI: 10.1021/acs.jmedchem.0c00231
  • Peer Reviewed / Open Access
• [Journal Article] Real-Time In-Cell NMR Reveals the Intracellular Modulation of GTP-Bound Levels of RAS (2020)
  • Author(s): Zhao Qingci、Fujimiya Ryu、Kubo Satoshi、Marshall Christopher B.、Ikura Mitsuhiko、Shimada Ichio、Nishida Noritaka
  • Journal Title: Cell Reports Volume: 32 Issue: 8 Pages: 108074-108074
  • DOI: 10.1016/j.celrep.2020.108074
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Targeting the cryptic sites: NMR-based strategy to improve protein druggability by controlling the conformational equilibrium (2020)
  • Author(s): Mizukoshi Yumiko、Takeuchi Koh、Tokunaga Yuji、Matsuo Hitomi、Imai Misaki、Fujisaki Miwa、Kamoshida Hajime、Takizawa Takeshi、Hanzawa Hiroyuki、Shimada Ichio
  • Journal Title: Science Advances Volume: 6 Issue: 40
  • DOI: 10.1126/sciadv.abd0480
  • Peer Reviewed / Open Access
• [Journal Article] Conformational equilibrium shift underlies altered K+ channel gating as revealed by NMR (2020)
  • Author(s): Iwahashi Yuta、Toyama Yuki、Imai Shunsuke、Itoh Hiroaki、Osawa Masanori、Inoue Masayuki、Shimada Ichio
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 5168-5168
  • DOI: 10.1038/s41467-020-19005-3
  • Peer Reviewed / Open Access
• [Journal Article] In situ structural biology using in-cell NMR (2020)
  • Author(s): Nishida Noritaka、Ito Yutaka、Shimada Ichio
  • Journal Title: Biochimica et Biophysica Acta (BBA) - General Subjects Volume: 1864 Issue: 2 Pages: 129364-129364
  • DOI: 10.1016/j.bbagen.2019.05.007
  • Peer Reviewed
• [Journal Article] Structural equilibrium underlying ligand-dependent activation of b2-adrenoreceptor (2020)
  • Author(s): Shunsuke Imai, Tomoki Yokomizo, Yutaka Kofuku, Yutaro Shiraishi, Takumi Ueda, Ichio Shimada
  • Journal Title: Nature Chemical Biology Volume: 16 Issue: 4 Pages: 430-439
  • DOI: 10.1038/s41589-019-0457-5
  • Peer Reviewed
• [Journal Article] Targeting FROUNT with disulfiram suppresses macrophage accumulation and its tumor-promoting properties (2020)
  • Author(s): Yuya Terashima, Etsuko Toda, Kouji Matsushima et al.
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 1-16
  • DOI: 10.1038/s41467-020-14338-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural basis for two-way communication between dynein and microtubules (2020)
  • Author(s): Nishida Noritaka、Komori Yuta、Takarada Osamu、Watanabe Atsushi、Tamura Satoko、Kubo Satoshi、Shimada Ichio、Kikkawa Masahide
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 1038-1038
  • DOI: 10.1038/s41467-020-14842-8
  • Peer Reviewed / Open Access
• [Journal Article] Activation of adenosine A2A receptor by lipids from docosahexaenoic acid revealed by NMR (2020)
  • Author(s): Takuya Mizumura, Keita Kondo, Masatoshi Kurita, Yutaka Kofuku, Mei Natsume, Shunsuke Imai, Yutaro Shiraishi, Takumi Ueda, Ichio Shimada
  • Journal Title: Science Advances Volume: 6 Issue: 12
  • DOI: 10.1126/sciadv.aay8544
  • Peer Reviewed / Open Access
• [Journal Article] Structural mechanism underlying G protein family-specific regulation of G protein-gated inwardly rectifying potassium channel (2019)
  • Author(s): Kano Hanaho、Toyama Yuki、Imai Shunsuke、Iwahashi Yuta、Mase Yoko、Yokogawa Mariko、Osawa Masanori、Shimada Ichio
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 1-13
  • DOI: 10.1038/s41467-019-10038-x
  • Peer Reviewed / Open Access
• [Journal Article] Function-related conformational dynamics of G protein-coupled receptors revealed by NMR (2019)
  • Author(s): Takumi Ueda, Yutaka Kofuku, Junya Okude, Shunsuke Imai, Yutaro Shiraishi, Ichio Shimada
  • Journal Title: Biophysical Reviews Volume: 11 Issue: 3 Pages: 409-418
  • DOI: 10.1007/s12551-019-00539-w
  • Peer Reviewed / Open Access
• [Journal Article] Frequency selective coherence transfer NMR spectroscopy to study the structural dynamics of high molecular weight proteins (2019)
  • Author(s): Toyama Yuki、Shimada Ichio
  • Journal Title: Journal of Magnetic Resonance Volume: 304 Pages: 62-77
  • DOI: 10.1016/j.jmr.2019.05.004
  • Peer Reviewed
• [Journal Article] Decreased conformational stability in the oncogenic N92I mutant of Ras-related C3 botulinum toxin substrate 1 (2019)
  • Author(s): Toyama Y, Kontani K, Katada T, Shimada I.
  • Journal Title: Sci. Adv. Volume: 5 Issue: 8
  • DOI: 10.1126/sciadv.aax1595
  • Peer Reviewed / Open Access
• [Journal Article] Conformational equilibrium defines the variable induction of the multidrug-binding transcriptional repressor QacR (2019)
  • Author(s): Koh Takeuchi, Misaki Imai, and Ichio Shimada
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 116 Issue: 40 Pages: 19963-19972
  • DOI: 10.1073/pnas.1906129116
  • Peer Reviewed
• [Journal Article] NMR 解析の最前線：分子間相互作用と動的構造 (2019)
  • Author(s): 上田卓見、嶋田一夫
  • Journal Title: 月刊細胞 Volume: 51 Pages: 596-599
• [Journal Article] NMR Studies of Function-related Conformational Equilibria of GPCRs (2019)
  • Author(s): 上田卓見、嶋田一夫
  • Journal Title: Seibutsu Butsuri Volume: 59 Issue: 4 Pages: 181-187
  • DOI: 10.2142/biophys.59.181
  • NAID: 130007683541
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed
• [Journal Article] 夾雑系の構造生物学 (2019)
  • Author(s): 西田紀貴、嶋田一夫
  • Journal Title: 化学と工業 Volume: 72 Pages: 404-406
• [Journal Article] Conformational landscape alternations promote oncogenic activities of Ras-related C3 botulinum toxin substrate 1 as revealed by NMR (2019)
  • Author(s): Toyama Yuki、Kontani Kenji、Katada Toshiaki、Shimada Ichio
  • Journal Title: Science Advances Volume: 5 Issue: 3
  • DOI: 10.1126/sciadv.aav8945
  • Peer Reviewed / Open Access
• [Journal Article] 特集 いかに創薬を進めるか NMRで標的タンパク質の機能から創薬を (2018)
  • Author(s): 上田 卓見、嶋田 一夫
  • Journal Title: 生体の科学 Volume: 69 Issue: 4 Pages: 315-319
  • DOI: 10.11477/mf.2425200823
  • ISSN: 0370-9531, 1883-5503
  • Year and Date: 2018-08-15
• [Journal Article] GPCR drug discovery: integrating solution NMR data with crystal and cryo-EM structures (2018)
  • Author(s): Shimada Ichio、Ueda Takumi、Kofuku Yutaka、Eddy Matthew T.、Wuthrich Kurt
  • Journal Title: Nature Reviews Drug Discovery Volume: 18 Issue: 1 Pages: 59-82
  • DOI: 10.1038/nrd.2018.180
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Application of C-terminal 7-azabicyclo[2.2.1]heptane to stabilize β?strand-like extended conformation of a neighboring α-amino acid (2018)
  • Author(s): Luhan Zhai et al
  • Journal Title: J. Org. Chem., Volume: 83 Issue: 21 Pages: 13063-13079
  • DOI: 10.1021/acs.joc.8b01756
  • Peer Reviewed
• [Journal Article] Phosphoinositide binding by the PH domain in ceramide transfer protein (CERT) is inhibited by hyperphosphorylation of an adjacent serine-repeat motif (2018)
  • Author(s): Sugiki Toshihiko、Egawa Daichi、Kumagai Keigo、Kojima Chojiro、Fujiwara Toshimichi、Takeuchi Koh、Shimada Ichio、Hanada Kentaro、Takahashi Hideo
  • Journal Title: Journal of Biological Chemistry Volume: 293 Issue: 28 Pages: 11206-11217
  • DOI: 10.1074/jbc.ra118.002465
  • Peer Reviewed / Open Access
• [Journal Article] Endogenous Membrane Receptor Labeling by Reactive Cytokines and Growth Factors to Chase Their Dynamics in Live Cells (2018)
  • Author(s): Takaoka Yousuke、Uchinomiya Shohei、Kobayashi Daichi、Endo Masataka、Hayashi Takahiro、Fukuyama Yoshiaki、Hayasaka Haruko、Miyasaka Masayuki、Ueda Takumi、Shimada Ichio、Hamachi Itaru
  • Journal Title: Chem Volume: 4 Issue: 6 Pages: 1451-1464
  • DOI: 10.1016/j.chempr.2018.03.021
  • Peer Reviewed / Open Access
• [Journal Article] Phosphorylation-induced conformation of β2-adrenoceptor related to arrestin recruitment revealed by NMR (2018)
  • Author(s): Yutaro Shiraishi, Mei Natsume, Yutaka Kofuku, Shunsuke Imai, Kunio Nakata, Toshimi Mizukoshi, Takumi Ueda, Hideo Iwai, Ichio Shimada
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 164-164
  • DOI: 10.1038/s41467-017-02632-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural basis for the ethanol action on G-protein?activated inwardly rectifying potassium channel 1 revealed by NMR spectroscopy (2018)
  • Author(s): Toyama Yuki、Kano Hanaho、Mase Yoko、Yokogawa Mariko、Osawa Masanori、Shimada Ichio
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 115 Issue: 15 Pages: 3858-3863
  • DOI: 10.1073/pnas.1722257115
  • Peer Reviewed
• [Journal Article] Balanced Regulation of Redox Status of Intracellular Thioredoxin Revealed by in-Cell NMR (2018)
  • Author(s): Mochizuki Ayano、Saso Arata、Zhao Qingci、Kubo Satoshi、Nishida Noritaka、Shimada Ichio
  • Journal Title: Journal of the American Chemical Society (selected as JACS Spotlights) Volume: 140 Issue: 10 Pages: 3784-3790
  • DOI: 10.1021/jacs.8b00426
  • Peer Reviewed
• [Journal Article] Deuteration and Selective Labeling of Alanine Methyl Groups of β 2-Adrenergic Receptor Expressed in a Baculovirus-Insect Cell Expression System (2018)
  • Author(s): Yutaka Kofuku, Tomoki Yokomizo, Shunsuke Imai, Yutaro Shiraishi, Mei Natsume, Hiroaki Itoh, Masayuki Inoue, Kunio Nakata, Shunsuke Igarashi, Hideyuki Yamaguchi, Toshimi Mizukoshi, Ei-Ichiro Suzuki, Takumi Ueda, Ichio Shimada
  • Journal Title: Journal of Biomolecular NMR Volume: 印刷中 Issue: 3 Pages: 185-192
  • DOI: 10.1007/s10858-018-0174-5
  • Peer Reviewed / Open Access
• [Journal Article] Functional roles of Mg2+ binding sites in ion-dependent gating of a Mg2+ channel, MgtE, revealed by solution NMR (2018)
  • Author(s): Maruyama Tatsuro、Imai Shunsuke、Kusakizako Tsukasa、Hattori Motoyuki、Ishitani Ryuichiro、Nureki Osamu、Ito Koichi、Maturana Andr?s D、Shimada Ichio、Osawa Masanori
  • Journal Title: eLife Volume: 7
  • DOI: 10.7554/elife.31596
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of the multimeric structure of poly(A)-binding protein on a poly(A) tail (2018)
  • Author(s): Sawazaki Ryoichi、Imai Shunsuke、Yokogawa Mariko、Hosoda Nao、Hoshino Shin-ichi、Mio Muneyo、Mio Kazuhiro、Shimada Ichio、Osawa Masanori
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 1455-1455
  • DOI: 10.1038/s41598-018-19659-6
  • Peer Reviewed / Open Access
• [Journal Article] Dynamic domain arrangement of CheA-CheY complex regulates bacterial thermotaxis, as revealed by NMR (2017)
  • Author(s): YuYuichi Minato, Takumi Ueda, Asako Machiyama, Hideo Iwai, Ichio Shimada
  • Journal Title: as revealed by NMR Scientific Reports Volume: 7 Issue: 1 Pages: 16462-16462
  • DOI: 10.1038/s41598-017-16755-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Forbidden Coherence Transfer of 19F Nuclei to Quantitatively Measure the Dynamics of a CF3-Containing Ligand in Receptor-Bound States (2017)
  • Author(s): Tokunaga Yuji、Takeuchi Koh、Shimada Ichio
  • Journal Title: Molecules Volume: 22 Issue: 9 Pages: 1492-1492
  • DOI: 10.3390/molecules22091492
  • Peer Reviewed / Open Access
• [Journal Article] ATP-dependent modulation of MgtE in Mg2+ homeostasis (2017)
  • Author(s): Tomita Atsuhiro、Zhang Mingfeng、Jin Fei、Zhuang Wenhui、Takeda Hironori、Maruyama Tatsuro、Osawa Masanori、Hashimoto Ken-ichi、Kawasaki Hisashi、Ito Koichi、Dohmae Naoshi、Ishitani Ryuichiro、Shimada Ichio、Yan Zhiqiang、Hattori Motoyuki、Nureki Osamu
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 148-148
  • DOI: 10.1038/s41467-017-00082-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A small-molecule compound inhibits a collagen-specific molecular chaperone and could represent a potential remedy for fibrosis (2017)
  • Author(s): S. Ito, K. Ogawa, K. Takeuchi, M. Takagi, M. Yoshida, T. Hirokawa, S. Hirayama, K. Shin-Ya, I. Shimada, T. Doi, N. Goshima, T. Natsume, K. Nagata
  • Journal Title: J. Biol. Chem. Volume: 292 Issue: 49 Pages: 20076-20085
  • DOI: 10.1074/jbc.m117.815936
  • Peer Reviewed / Open Access
• [Journal Article] Nuclear Magnetic Resonance Approaches for Characterizing Protein-Protein Interactions (2017)
  • Author(s): Toyama Yuki、Mase Yoko、Kano Hanaho、Yokogawa Mariko、Osawa Masanori、Shimada Ichio
  • Journal Title: Methods in Molecular Biology (invited review) Volume: 1684 Pages: 115-128
  • DOI: 10.1007/978-1-4939-7362-0_10
  • ISBN: 9781493973613, 9781493973620
  • Peer Reviewed
• [Presentation] Function-Related Dynamics of Membrane Proteins by NMR (2021)
  • Author(s): Ichio Shimada
  • Organizer: Xingda Lecture
  • Invited
• [Presentation] Function-related Dynamics of GPCRs (2021)
  • Author(s): Ichio Shimada
  • Organizer: ISMAR-APNMR-NMRSJ2021
  • Int'l Joint Research / Invited
• [Presentation] Function-related dynamics of GPCR by NMR (2021)
  • Author(s): Ichio Shimada
  • Organizer: Pacifichem
  • Int'l Joint Research / Invited
• [Presentation] Membrane protein function by NMR (2021)
  • Author(s): Ichio Shimada
  • Organizer: PDB 50th Anniversary Symposium in Asia
  • Int'l Joint Research / Invited
• [Presentation] Targeting cryptic sites: NMR-based strategy to improve protein druggability by controlling conformational equilibrium (2021)
  • Author(s): Koh Takeuchi
  • Organizer: ISMAR-APNMR-NMRSJ2021
  • Int'l Joint Research / Invited
• [Presentation] Ras阻害環状ペプチドの構造的柔軟性が決める細胞内移行性 (2021)
  • Author(s): 竹内恒
  • Organizer: 第59回 生物物理学会
  • Invited
• [Presentation] 溶液 NMR による多様な創薬モダリティに対する動的構造創薬 (2021)
  • Author(s): 竹内恒
  • Organizer: 第21回日本蛋白質科学会年会
  • Invited
• [Presentation] 細胞内生命現象をリアルタイム観測するNMR法の開発と応用 (2021)
  • Author(s): 西田紀貴
  • Organizer: ISMAR-APNMR-NMRSJ2021
  • Invited
• [Presentation] 分子夾雑環境が変調するタンパク質の構造と機能 (2021)
  • Author(s): 西田紀貴
  • Organizer: 第21回蛋白質科学会年会
  • Invited
• [Presentation] Function-related Dynamics of G-protein-coupled Receptors (2021)
  • Author(s): Ichio Shimada
  • Organizer: RIKEN BDR Symposium
  • Int'l Joint Research / Invited
• [Presentation] Structural equilibrium underlying ligand-dependent activation of GPCR (2020)
  • Author(s): Ichio Shimada
  • Organizer: "Emerging Topics in Biomolecular Magnetic Resonance" seminar
  • Int'l Joint Research / Invited
• [Presentation] in situ function-related dynamical analyses of proteins (2020)
  • Author(s): 嶋田一夫
  • Organizer: 第59回 NMR討論会(2020)
  • Invited
• [Presentation] in situ 動的構造からみたタンパク質の機能解明と超高磁場への期待 (2020)
  • Author(s): 嶋田一夫
  • Organizer: よこはまNMR研究会 第65回ワークショップ
  • Invited
• [Presentation] 試験管内と細胞内での蛋白質の活性の違いを定量するin-cell NMRの開発 (2020)
  • Author(s): 西田紀貴
  • Organizer: 新学術領域「分子夾雑化学」オンラインセミナー
  • Invited
• [Presentation] NMRを用いた動的構造解析への取り組みと創薬への展開 (2020)
  • Author(s): 竹内恒
  • Organizer: 第59回 NMR 討論会
  • Invited
• [Presentation] NMRを用いた動的構造解析による多剤耐性機構の理解 (2020)
  • Author(s): 竹内恒
  • Organizer: 第5回トランスポーター研究会関東部会（JTRAK2020）
  • Invited
• [Presentation] Function-related dynamics of membrane proteins (2019)
  • Author(s): 嶋田一夫
  • Organizer: 日本薬学会・韓国薬学会合同シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] Function-related dynamics of proteins -G protein signaling- (2019)
  • Author(s): 嶋田一夫
  • Organizer: The 8th Asia-Pacific NMR Symposium
  • Int'l Joint Research / Invited
• [Presentation] NMR for high-molecular-weight proteins (2019)
  • Author(s): 嶋田一夫
  • Organizer: 第58回NMR討論会
  • Invited
• [Presentation] Function-related dynamics of GPCR by NMR (2019)
  • Author(s): 嶋田一夫
  • Organizer: 第100回日本化学会
  • Invited
• [Presentation] Conductance of P2X4 receptor is determined by conformational equilibrium in the transmembrane region (2019)
  • Author(s): Takumi Ueda, Ichio Shimada
  • Organizer: The 8th Asia-Pacific NMR symposium
  • Int'l Joint Research / Invited
• [Presentation] Conformational equilibrium defines variable transcriptional repression of a multidrug binding transcriptional repressor, QacR (2019)
  • Author(s): Koh Takeuchi, Imai Misaki, and Ichio Shimada
  • Organizer: The 8th Asia-Pacific NMR symposium
  • Int'l Joint Research / Invited
• [Presentation] 構造平衡制御によるタンパク質のドラッガビリティの改善 (2019)
  • Author(s): 竹内恒,水越弓子,德永裕二,滝沢剛,半沢 宏之、嶋田一夫
  • Organizer: 第58回NMR討論会
• [Presentation] Targeting the cryptic sites: NMR-based strategy to improve the draggability of proteins by controlling the conformational equilibrium (2019)
  • Author(s): Koh Takeuchi, Yumiko Mizukoshi, Yuji Tokunaga, Hitomi Matsuo, Takeshi Takizawa, Hiroyuki Hanzawa, and Ichio Shimada.
  • Organizer: 第57 回日本生物物理学会年会
• [Presentation] Conformational equilibrium defines transcriptional repression of multidrug binding transcriptional repressors (2019)
  • Author(s): 竹内恒,水越弓子,德永裕二,滝沢剛,半沢 宏之、嶋田一夫
  • Organizer: 日本分子生物学会年会 2019
  • Invited
• [Presentation] Real-time observation of the biological events within living cells using in-cell NMR (2019)
  • Author(s): Noritaka Nishida, Ichio Shimada
  • Organizer: The 8th Asia-Pacific NMR symposium
  • Int'l Joint Research / Invited
• [Presentation] NMRとクライオ電顕を用いた細胞質ダイニンの微小管親和性制御機構の解明 (2019)
  • Author(s): 西田紀貴
  • Organizer: 第20回若手NMR研究会
  • Invited
• [Presentation] 細胞内非1：1相互作用によるタンパク質活性制御機構のin-cell NMR観測 (2019)
  • Author(s): 西田紀貴
  • Organizer: 第42回分子生物学会年会
  • Invited
• [Presentation] Function-related dynamics of membrane proteins (2019)
  • Author(s): Ichio Shimada
  • Organizer: PSJ-PSK Joint Symposium, Anual meeting of PSJ
  • Int'l Joint Research / Invited
• [Presentation] in-cell NMR法を用いた細胞内生命反応のリアルタイム観測 (2019)
  • Author(s): 西田紀貴 嶋田一夫
  • Organizer: よこはまNMR研究会 第62回ワークショップ
  • Invited
• [Presentation] バイオ計測とバイオITの融合による創薬プラットフォーム技術の構築とその展開 (2019)
  • Author(s): 竹内恒
  • Organizer: 平成30年度産技連 関東甲信越静地域部会 食品・バイオ分科会
  • Invited
• [Presentation] Gタンパク質共役型受容体のシグナル伝達制御機構の解明 (2019)
  • Author(s): 白石 勇太郎 夏目 芽依 幸福 裕 今井 駿輔 中田 國夫 水越 利巳 上田 卓見 岩井 秀夫 嶋田 一夫
  • Organizer: 日本薬学会第139年会
• [Presentation] Developments and applications of novel NMR methods to characterize function-related dynamics of high molecular weight proteins (2018)
  • Author(s): Ichio Shimada
  • Organizer: XLVII National Congress on Magnetic Resonance (プレナリー講演)
  • Int'l Joint Research / Invited
• [Presentation] Function-related dynamics of proteins-G protein and arrestins signaling by GPCR (2018)
  • Author(s): Ichio Shimada
  • Organizer: 2.International Conference on Magnetic Resonance in Biological Systems 2018 (プレナリー講演)
  • Int'l Joint Research / Invited
• [Presentation] Function-related dynamics of membrane proteins -G-protein coupled inwardly rectifying K+ channel- (2018)
  • Author(s): Ichio Shimada
  • Organizer: The 6th International Symposium on Drug Discovery and Design by NMR
  • Int'l Joint Research / Invited
• [Presentation] Function-related dynamics of membrane proteins -G-protein coupled inwardly rectifying K+ channel- (2018)
  • Author(s): Ichio Shimada
  • Organizer: 4.Asian Biophysics Symposium, Australian Society for Biophysic Meeting
  • Int'l Joint Research / Invited
• [Presentation] Function-related dynamics of membrane proteins -G-protein coupled inwardly rectifying K+ channel- (2018)
  • Author(s): Ichio Shimada
  • Organizer: 2018 Louis-Jeantet Symposium, Centre Medical Universitaire (CMU)
  • Int'l Joint Research / Invited
• [Presentation] Dynamic structure-guided optimization of ligand (2018)
  • Author(s): Koh Takeuchi
  • Organizer: The Israeli 2018 Magnetic Resonance Meeting
  • Int'l Joint Research / Invited
• [Presentation] Dynamic structure-guided optimization of ligand using FCT method (2018)
  • Author(s): Koh Takeuchi, Yuji Tokunaga, Yumiko Mizukoshi, Ichio Shimada
  • Organizer: International Conference on Magnetic Resonance in Biological Systems 2018
  • Int'l Joint Research / Invited
• [Presentation] Dynamics of Membrane Proteins, -gating mechanism of the ethanol-induced activation of GIRK- (2018)
  • Author(s): Ichio Shimada
  • Organizer: The 18th World Congress of Basic and Clinical Pharmacology, WCP201
  • Int'l Joint Research / Invited
• [Presentation] Mechanism of multidrug resistance transcriptional regulation revealed by dynamic analysis using NMR (2018)
  • Author(s): Koh Takeuchi
  • Organizer: The Protein Society 32nd Annual symposium
  • Int'l Joint Research / Invited
• [Presentation] Developments and applications of novel NMR methods to characterize function-related dynamics of high molecular weight proteins (2018)
  • Author(s): Ichio Shimada
  • Organizer: Advanced Biological Chemistry Mini-symposium, Institute of Biological Chemistry
  • Int'l Joint Research / Invited
• [Presentation] in-cell NMR法を用いた細胞内生命現象のリアルタイム観測 (2018)
  • Author(s): 西田紀貴 嶋田一夫
  • Organizer: 日本分子生物学会第41回年会
  • Invited
• [Presentation] 禁制コヒーレント遷移を用いたリガンドの動的構造最適化 (2018)
  • Author(s): 竹内恒 水越弓子 德永裕二 滝沢剛 半沢宏之 嶋田一夫
  • Organizer: 第57回NMR討論会（誌上開催）
  • Invited
• [Presentation] 構造平衡により規定される多剤結合転写因子QacRの可変的転写制御 (2018)
  • Author(s): 竹内恒 嶋田一夫
  • Organizer: 第56回生物物理学会年会
  • Invited
• [Presentation] 多剤耐性転写制御の動的構造機能解析 (2018)
  • Author(s): 竹内恒 嶋田一夫
  • Organizer: 第18回蛋白質科学会年会
  • Invited
• [Presentation] 区分同位体標識を用いたβ2アドレナリン受容体のリン酸化によるシグナル制御機構の解明 (2018)
  • Author(s): 白石 勇太郎 夏目 芽依 幸福 裕 今井 駿輔 中田 國夫 水越 利巳 上田 卓見 岩井 秀夫 嶋田 一夫
  • Organizer: 第91回日本生化学会大会
• [Presentation] β2アドレナリン受容体のリン酸化によるシグナル制御機構の解明 (2018)
  • Author(s): 夏目 芽依 白石 勇太郎 幸福 裕 今井 駿輔 中田 國夫 水越 利巳 上田 卓見 岩井 秀夫 嶋田 一夫
  • Organizer: 第91回日本生化学会大会
• [Presentation] カリウムチャネルのミリ秒オーダーの開閉の構造機構の解明 (2018)
  • Author(s): 岩橋 優太、外山 侑樹、今井 駿輔、大澤 匡範、嶋田 一夫
  • Organizer: 第91回日本生化学会大会
• [Presentation] Gタンパク質のファミリー選択的なK+チャネル活性制御機構の構造生物学的解明 (2018)
  • Author(s): 加納 花穂、外山 侑樹、今井 駿輔、岩橋 優太、間瀬 瑤子、横川 真梨子、大澤 匡範、嶋田 一夫
  • Organizer: 第91回日本生化学会大会
• [Presentation] G タンパク質共役型内向き整流性カリウムチャネルのエタノール依存的な活性化機構の解明 (2018)
  • Author(s): 外山 侑樹 加納 花穂 間瀬 瑶子 横川 真梨子 大澤 匡範 嶋田 一夫
  • Organizer: 第57回NMR討論会（誌上開催）
• [Presentation] 昆虫細胞発現系における安定同位体標識法の開発とGタンパク質共役型受容体のNMR解析 (2018)
  • Author(s): 幸福 裕、上田 卓見、奥出 順也、白石 勇太郎、夏目 芽依、横溝 智貴、今井 俊輔、近藤 啓太、水村 拓也、鈴木 志歩、伊藤 寛晃、井上 将行、中田 國夫、五十嵐 俊介、山口 秀幸、水越 利巳、鈴木 栄一郎、嶋田 一夫
  • Organizer: 第20回若手NMR研究会
• [Presentation] ゲノム編集技術を活用した細胞内Rasの活性制御機構のin-cell NMR観測 (2018)
  • Author(s): Qingci Zhao, 藤宮璃勇, Chris Marshall, 伊倉光彦, 西田紀貴, 嶋田一夫
  • Organizer: 第16回次世代を担う若手のためのフィジカル・ファーマフォーラム(PPF2018）
• [Presentation] Studying Proteins inside Eukaryotic Cells in NMR (2018)
  • Author(s): 嶋田一夫
  • Organizer: The 62nd Annual Meeting Biophysical Society, San Francisco
  • Int'l Joint Research / Invited
• [Presentation] NMRによる膜タンパク質のin situ機能解明 (2018)
  • Author(s): 嶋田一夫
  • Organizer: 機能タンパク質の構造と機能のダイナミクスとそれに基づく細胞・生体システム作動機構の研究拠点の形成、生理研
  • Invited
• [Presentation] NMRによる膜タンパク質のin situ機能解明 (2018)
  • Author(s): 嶋田一夫
  • Organizer: 平成29年度第2回生物構造学研究会、神楽坂
  • Invited
• [Presentation] NMR法により解明した、マルチドメイン蛋白質複合体CheA-CheYの動的ドメイン間相互作用による走温性制御機構 (2018)
  • Author(s): 上田卓見、湊雄一、町山麻子、岩井秀夫、嶋田一夫
  • Organizer: 日本薬学会第138年会
• [Presentation] 昆虫細胞発現系におけるアラニン選択的安定同位体標識法の開発と脂質二重膜中のβ2-アドレナリン受容体への応用 (2018)
  • Author(s): 中田國夫、五十嵐俊介、幸福裕、上田卓見、白石勇太郎、夏目芽依、横溝智貴、今井俊輔、水越利巳、鈴木榮一郎、嶋田一夫
  • Organizer: 日本薬学会第138年会
• [Presentation] アラニン側鎖メチル基を観測対象としたβ2-アドレナリン受容体のシグナル伝達機構の解明 (2018)
  • Author(s): 横溝智貴、今井俊輔、幸福裕、上田卓見、白石勇太郎、夏目芽依、中田國夫、五十嵐俊介、水越利巳、鈴木榮一郎、嶋田一夫
  • Organizer: 日本薬学会第138年会
• [Presentation] β2アドレナリン受容体のリン酸化によるシグナル制御機構の解明 (2018)
  • Author(s): 夏目芽依、白石勇太郎、幸福裕、今井駿輔、中田國夫、水越利巳、上田卓見、岩井秀夫、嶋田一夫
  • Organizer: 日本薬学会第138年会
• [Presentation] 区分同位体標識を用いたβ2アドレナリン受容体のリン酸化によるシグナル制御機構の解明 (2018)
  • Author(s): 白石勇太郎、夏目芽依、幸福裕、今井駿輔、中田國夫、水越利巳、上田卓見、岩井秀夫、嶋田一夫
  • Organizer: 日本薬学会第138年会
• [Presentation] Real-time observation of the biological events within living cells using in-cell NMR (2018)
  • Author(s): 西田紀貴、嶋田一夫
  • Organizer: JST-Mirai symposium
  • Int'l Joint Research / Invited
• [Presentation] 酸化ストレスに対する細胞内Trxの細胞応答のin-cell NMR観測 (2018)
  • Author(s): 望月綾野、佐宗新、久保智史、西田紀貴、嶋田一夫
  • Organizer: 日本薬学会第138年会
• [Presentation] in-cell NMR法による細胞内環境下におけるRasの活性状態の評価 (2018)
  • Author(s): 趙慶慈、藤宮璃勇、Chris Marshall、伊倉光彦、西田紀貴、嶋田一夫
  • Organizer: 日本薬学会第138年会
• [Presentation] Functional dynamics of membrane proteins by NMR (2017)
  • Author(s): 嶋田一夫
  • Organizer: Department Seminar, The Scripps Research Institute, La Jolla
  • Invited
• [Presentation] 核磁気共鳴法による膜タンパク質のin situ 機能解明 (2017)
  • Author(s): 嶋田一夫
  • Organizer: DDSBシンポジウム 構造生命科学のフロンティア、理研
  • Invited
• [Presentation] Functional dynamics of membrane proteins by NMR (2017)
  • Author(s): 嶋田一夫
  • Organizer: KMRS Award Lecture, Koran Magnetc Resonance Society Annual Meeing, Busan
  • Int'l Joint Research / Invited
• [Presentation] Developments and applications of multiple quantum NMR methods to characterize functional dynamics of high molecular weight proteins (2017)
  • Author(s): 嶋田一夫
  • Organizer: The 5th Conference of the Asia Pacific Protein, Bangsean
  • Int'l Joint Research / Invited
• [Presentation] Functional dynamics of proteins by NMR (2017)
  • Author(s): 嶋田一夫
  • Organizer: The Protein Society 31st Annual Symposium, Montreal
  • Int'l Joint Research / Invited
• [Presentation] 核磁気共鳴法によるタンパク質のin situ 機能解明 (2017)
  • Author(s): 嶋田一夫
  • Organizer: NMR共用プラットフォームシンポジウム -高磁場NMRを核としたプラットフォームの新たな展開-, 理研
  • Invited
• [Presentation] Functional dynamics of proteins by NMR (2017)
  • Author(s): 嶋田一夫
  • Organizer: J-PARK international symposium, Tokai
  • Int'l Joint Research / Invited
• [Presentation] Developments and applications of novel NMR methods to characterize functional dynamics of high molecular weight proteins (2017)
  • Author(s): 嶋田一夫
  • Organizer: 2017 Taiwan-Japan Biomedical Symposium on Magnetic Resonance、Tainan
  • Int'l Joint Research / Invited
• [Presentation] Function-related dynamics of membrane proteins by NMR (2017)
  • Author(s): 嶋田一夫
  • Organizer: The 4th membrane protein workshop 2017, Taipei
  • Int'l Joint Research / Invited
• [Presentation] Developments and applications of novel NMR methods to characterize functional dynamics of high molecular weight proteins (2017)
  • Author(s): 嶋田一夫
  • Organizer: The 15th Chinese Biophysics Congress, Shanghai
  • Int'l Joint Research / Invited
• [Presentation] Functional dynamics of membrane proteins revealed by NMR (2017)
  • Author(s): 上田卓見、嶋田一夫
  • Organizer: 蛋白質科学会
  • Invited
• [Presentation] 安定同位体標識法の応用による、蛋白質の動的構造の解明 (2017)
  • Author(s): 上田卓見、嶋田一夫
  • Organizer: 第56回NMR討論会
  • Invited
• [Presentation] 動的構造に着目したGタンパク質共役型受容体の機能解明 (2017)
  • Author(s): 幸福裕、上田卓見、嶋田一夫
  • Organizer: 第61回日本薬学会関東支部大会
  • Invited
• [Presentation] 動的構造解析による多剤耐性転写制御因子の非特異的かつ高親和性な結合メカニズムの解明 (2017)
  • Author(s): 竹内 恒、今井 美咲、德永 裕二、嶋田 一夫
  • Organizer: 第55回生物物理学会年会
  • Invited
• [Presentation] Mechanism of multidrug resistance transcriptional regulation revealed by quantitative dynamic analysis using site-specific isotope labeling (2017)
  • Author(s): 竹内 恒、嶋田 一夫
  • Organizer: 第56回日本核磁気共鳴学会年会
  • Invited
• [Presentation] カリウムチャネルのミリ秒オーダーの開閉の構造機構の解明 (2017)
  • Author(s): 岩橋 優太、外山 侑樹、今井 駿輔、大澤 匡範、嶋田 一夫
  • Organizer: 第17回日本蛋白質科学会年会
• [Presentation] Gタンパク質のファミリー選択的なK+チャネル活性制御機構の解明 (2017)
  • Author(s): 加納 花穂, 外山 侑樹, 岩橋 優太, 間瀬 瑶子, 横川 真梨子, 大澤 匡範, 嶋田 一夫
  • Organizer: 第17回日本蛋白質科学会年会
• [Presentation] Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses (2017)
  • Author(s): Hanaho Kano, Yuki Toyama, Yoko Mase, Mariko Yokogawa, Masanori Osawa, and Ichio Shimada
  • Organizer: 2017 Taiwan-Japan Biomedical Symposium on Magnetic Resonance
  • Int'l Joint Research
• [Presentation] 緩和速度の磁場依存性解析によるGタンパク質へのGDP結合の動的制御機構の解明 (2017)
  • Author(s): 外山 侑樹，加納花穂, 間瀬瑤子, 横川真梨子, 大澤匡範，嶋田一夫
  • Organizer: 第56回NMR討論会
• [Presentation] Real-time observation of the biological events within living cells using in-cell NMR (2017)
  • Author(s): 西田紀貴、嶋田一夫
  • Organizer: 第17回日本蛋白質科学会
  • Invited
• [Presentation] Real-time observation of the biological events within living cells using in-cell NMR (2017)
  • Author(s): 西田紀貴、嶋田一夫
  • Organizer: ConBio2017
  • Invited
• [Presentation] in-cell NMR法による細胞内環境下におけるRasの活性状態の評価 (2017)
  • Author(s): 趙慶慈、藤宮璃勇、Chris Marshall、伊倉光彦、西田紀貴、嶋田一夫
  • Organizer: ConBio2017
• [Remarks] 理化学研究所 生命機能科学研究センター 生体分子動的構造研究チーム
  • URL: https://www.bdr.riken.jp/jp/research/labs/shimada-i/index.html
• [Remarks] 東京大学薬学部 生命物理化学教室ホームページ
  • URL: https://biophys.f.u-tokyo.ac.jp/
• [Remarks] 東京大学薬学部 生命物理化学教室ホームページ
  • URL: http://ishimada.f.u-tokyo.ac.jp/public_html/index_j.html
• [Remarks] 東京大学薬学部生命物理化学教室ホームページ
  • URL: http://ishimada.f.u-tokyo.ac.jp/public_html/index_j.html