Budget Amount *help |
¥153,920,000 (Direct Cost: ¥118,400,000、Indirect Cost: ¥35,520,000)
Fiscal Year 2021: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
Fiscal Year 2020: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2019: ¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
Fiscal Year 2018: ¥35,880,000 (Direct Cost: ¥27,600,000、Indirect Cost: ¥8,280,000)
Fiscal Year 2017: ¥36,140,000 (Direct Cost: ¥27,800,000、Indirect Cost: ¥8,340,000)
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Outline of Final Research Achievements |
In this research, starting from the scientific elucidation of Chinese hamster ovary (CHO) cells as mammalian cells for industrial use, we performed integrated research based on cell and bioprocess engineering. We also conducted research on the basic research on integrated bioprocesses, including the improvement of productivity by enhancing the expression of multiple factors related to intracellular protein secretion, the evaluation of artificial induction of chromosomal aneuploidy and its effects, the construction of sub-strains showing 5-10 times higher productivity with various antibody molecules and the analysis of factors affecting productivity, the discovery of suitable perfusion rate per cell and appropriate media composition ratio in continuous perfusion culture using cell lines derived from Chinese hamsters with fast growth rate. Finally, we constructed the fundamental research on integrated bioprocesses for industrial mammalian cells.
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