| Budget Amount *help |
¥203,060,000 (Direct Cost: ¥156,200,000、Indirect Cost: ¥46,860,000)
Fiscal Year 2021: ¥40,170,000 (Direct Cost: ¥30,900,000、Indirect Cost: ¥9,270,000)
Fiscal Year 2020: ¥38,740,000 (Direct Cost: ¥29,800,000、Indirect Cost: ¥8,940,000)
Fiscal Year 2019: ¥41,080,000 (Direct Cost: ¥31,600,000、Indirect Cost: ¥9,480,000)
Fiscal Year 2018: ¥51,740,000 (Direct Cost: ¥39,800,000、Indirect Cost: ¥11,940,000)
Fiscal Year 2017: ¥31,330,000 (Direct Cost: ¥24,100,000、Indirect Cost: ¥7,230,000)
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| Outline of Final Research Achievements |
To elucidate the mechanism of sexual differentiation of mouse germ cells, we elucidated the mechanism of action of the RNA-binding protein NANOS2, which is a central regulator of masculinization, and its partner factor DND1. Biochemical analysis using a cell reconstitution system and visualization of target RNA revealed that DND1 recognizes target RNA, then NANOS2 binds to the RNA-bound DND1 to ensure catching a specific target, and then NANOS2 recruits CNOT deadenylation complex leading to RNA degradation. We found candidate genes required for germline masculinization by single-cell analysis using sex-reversed germ cells. In addition, we constructed a system that can rapidly knock down the protein in mice (AID2 protein knockdown mouse), enabling direct functional analysis of the NANOS2 protein.
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