Researches on development of crustacean allergy

• Project/Area Number: 17H06248
• Research Category: Grant-in-Aid for Challenging Research (Pioneering)
• Allocation Type: Single-year Grants
• Research Field: Forestry and Forest Products Science, Applied aquatic science, and related fields
• Research Institution: The University of Tokyo
• Principal Investigator: Ushio Hideki 東京大学, 大学院農学生命科学研究科(農学部), 教授 (50251682)
• Project Period (FY): 2017-06-30 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000); Fiscal Year 2019: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000); Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
• Keywords: 甲殻類 / ヘモシアニン / トロポミオシン / 食物アレルギー / 炎症 / アレルギー / 感作 / 節足動物 / エビ・カニ類 / アレルゲン

Outline of Final Research Achievements

This study tests the hypothesis that crustacean food allergy should involve an IgE-mediated allergy and other mechanisms. Crustacean hemocyanin (CHM) induced inflammatory responses human THP-1 monocyte and macrophage cells. Dermal patch with CHM and allergen tropomyosin aggravated allergic responses of allergy model mouse. Phage display and bio-panning using THP-1 cells found that inflammatory peptide sequences would be included in many kinds of foods.

Academic Significance and Societal Importance of the Research Achievements

現在のように動物タンパク質源が確保できる状況では，「甲殻類を食べない」という選択が可能であるが，FAO が提唱するように将来的な動物タンパク質供給手段として昆虫食が重要となる可能性も高い．甲殻類アレルギー患者が非常に高い確率で同じ節足動物門に属する昆虫類にも強いアレルギー反応を示す事実から考えると，本研究で想定している甲殻類アレルギー患者は，昆虫の摂取ができない可能性が非常に高い．本研究の成果は，甲殻類の処理方時あるいは調理時に経皮感作をできるだけ予防する方策が有効であることを示している．これらは，昆虫食で支えるかもしれない将来的な食料対策の一助となる．

Research Products

• [Journal Article] tumor-suppressing potential of stingless bee propolis in in vitro and in vivo models of differentiated-type gastric adenocarcinoma (2019)
  • Author(s): Mark Joseph Desamero, Shigeru Kakuta, Yulan tang, James Kenn chambers, Kazuyuki Uchida, Maria Amelita estacio, Cleofas cervancia, Yuri Kominami, Hideki Ushio, Jun nakayama, Hiroyuki nakayama & Shigeru Kyuwa
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 19635-19635
  • DOI: 10.1038/s41598-019-55465-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Novel Analysis of the Peptide Terminome Characterizes Dynamics of Proteolytic Regulation in Vertebrate Skeletal Muscle Under Severe Stress (2019)
  • Author(s): Kominami Yuri、Hayashi Tatsuya、Tokihiro Tetsuji、Ushio Hideki
  • Journal Title: Proteomes Volume: 7 Issue: 1 Pages: 6-6
  • DOI: 10.3390/proteomes7010006
  • Peer Reviewed / Open Access
• [Journal Article] Application of magnetic resonance technologies in aquatic biology and seafood science (2019)
  • Author(s): Kaneko, Ushio, Ji
  • Journal Title: Fisheries Science Volume: 85 Issue: 1 Pages: 1-17
  • DOI: 10.1007/s12562-018-1266-6
  • NAID: 40021782055
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Changes in Intestinal Gene Expression of Zebrafish (Danio rerio) Related to Sterol Uptake and Excretion upon β-Sitosterol Administration (2018)
  • Author(s): M Takase, H Ushio
  • Journal Title: Fsihes Volume: 3 Issue: 1 Pages: 1-6
  • DOI: 10.3390/fishes3010001
  • Peer Reviewed / Open Access
• [Journal Article] Pre-harvest Modulation of N-3 Long-chain Polyunsaturated Fatty Acids in Rainbow Trout Meat for Human Consumption (2018)
  • Author(s): H Yoshinaga, H Ushio, Y Haga, S Satoh
  • Journal Title: J Food Process Technol Volume: 9 Issue: 02 Pages: 716-720
  • DOI: 10.4172/2157-7110.1000716
  • Peer Reviewed / Open Access
• [Journal Article] Ferulic acid promotes hypertrophic growth of fast skeletal muscle in zebrafish model (2017)
  • Author(s): Y Wen, H Ushio
  • Journal Title: Nutrients Volume: 9 Issue: 10 Pages: 1066-1066
  • DOI: 10.3390/nu9101066
  • Peer Reviewed / Open Access
• [Journal Article] Genome sequencing andannotation of Aeromonas veronii strain Ae52, a multidrug- resistant isolate fromsepticaemic goldfish (Carassius auratus) in Sri Lanka (2017)
  • Author(s): S Jagoda, K Honein, A Arulkanthan, H Ushio, S Asakawa
  • Journal Title: Genomics data Volume: 11 Pages: 46-48
  • DOI: 10.1016/j.gdata.2016.11.011
  • Peer Reviewed / Open Access
• [Presentation] 光親和性標識法によるマウス脂肪細胞におけるフコース相互作用分子の探索 (2020)
  • Author(s): 中尾 友彦、小南 友里、渡邉 壮一、細谷 孝充、潮 秀樹
  • Organizer: 日本農芸化学会
• [Presentation] スピルリナでの15N部分置換状態におけるタンパク質ターンオーバーの数理モデリング (2019)
  • Author(s): 川幡，上林，小南，渡邊，潮
  • Organizer: 日本農芸化学会
• [Presentation] ミオシン重鎖におけるtransuglutaminase反応領域の網羅的探索 (2019)
  • Author(s): 三根，小南，渡邊，潮
  • Organizer: 日本農芸化学会
• [Presentation] Dynamics of proteolysis regulation in teleost skeletal muscle: a multiple linear regression analysis using quantitative peptidome (2017)
  • Author(s): Y Kominami, T Hayashi, T Tokihiro, H Ushio
  • Organizer: JSFS 85 th internatinal symposium Fisheries Science for Future Generations
  • Int'l Joint Research
• [Presentation] Effects of dietary amino acid-imbalance diet on lipid metabolism in fish (2017)
  • Author(s): H Yoshinaga, N Kabeya, H Ushio, Y Haga, S Satoh
  • Organizer: JSFS 85 th internatinal symposium Fisheries Science for Future Generations
  • Int'l Joint Research
• [Presentation] Application of stable isotope labeling in aquatic biochemistry (2017)
  • Author(s): G Kaneko, H Ushio
  • Organizer: JSFS 85 th internatinal symposium Fisheries Science for Future Generations
  • Int'l Joint Research / Invited