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Elucidation of the molecular mechanisms of apomixis in plants

Research Project

Project/Area Number 17H06256
Research Category

Grant-in-Aid for Challenging Research (Pioneering)

Allocation TypeSingle-year Grants
Research Field Biology of Cells to Organisms, and related fields
Research InstitutionSaitama University

Principal Investigator

TAKAGI MASARU  埼玉大学, 理工学研究科, 教授 (40357348)

Co-Investigator(Kenkyū-buntansha) 木下 哲  横浜市立大学, 木原生物学研究所, 教授 (60342630)
Project Period (FY) 2017-06-30 – 2020-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000)
Fiscal Year 2019: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2018: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2017: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Keywords転写因子 / アポミクシス / キメラリプレッサー / 転写制御 / 胚発生 / 胚乳発生 / 胚乳形成 / 胚形成 / 未受精 / 胚珠肥大 / 胚乳 / 胚 / シロイヌナズナ / インプリンティング / リプレッサー / エピジェネティック制御 / 遺伝子 / エピジェネシス
Outline of Final Research Achievements

Using the newly developed gene silencing technology (CRES-T), we isolated chimeric repressors that frequently induces adventitious embryos for the purpose of artificially inducing the apomixis phenomenon. As a result of expression in the embryonic endothelium, which is a maternal 2n tissue, we succeeded in isolating a transformant having two torpedo-type embryos, which are considered to be a fertilized embryo and an apomixis embryo, in one ovule at the same time. We also found a chimeric repressor for five transcription factors named ESP, which enlarges the endosperm without fertilization. It was revealed that rice plants expressing a chimeric repressor for these rice orthologs could be produced endosperm formation without fertilization.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果を基に、植物における受精と種子形成の制御機構の全容である「受精機構の謎」と、その進化的役割も解き明かすことができると考えられる。加えて、受精は子孫となる胚に遺伝的バリエーションをもたらし、生存戦略にとって有利になることは明白であるが、「植物の胚乳発生に受精は必須なのか」という問いに対しての解答を得ることができる。加えて本アポミクシス誘導技術をベースに、主要穀物でF1ハイブリッド種を固定できれば、20%の増産が見込まれ、増産分をバイオエタノール生産に流用した場合、主要穀物の世界レベルの一年間の生産量から概算して下2億トンCO2削減効果が期待できる。

Report

(4 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (20 results)

All 2021 2020 2019 2018 2017

All Presentation (16 results) (of which Int'l Joint Research: 5 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 2 results)

  • [Presentation] シロイヌナズナの胚乳で発現する転写因子ELONGATION OF SILIQUES WITHOUT POLLINATION 3 の解析2021

    • Author(s)
      高崎寛則, 池田美穂, 長谷川玲花, Yilin Zhang, 坂本真吾, 丸山大輔, 光田展隆, 木下哲, 高木優
    • Organizer
      第62回日本植物生理学会シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] Regulatory mechanisms of nutrient supply necessary for embryogenesis2021

    • Author(s)
      Hironori Takasaki, Miho Ikeda, Yillin Zhang, Shingo Sakamoto, Daisuke Maruyama, Nobutaka Mitsuda, Tetsu Kinoshita, Masaru Ohme-Takagi.
    • Organizer
      第61回日本植物生理学会シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] Induction of Somatic Embryogenesis, Auxin and Transcription Factors2020

    • Author(s)
      Miho Ikeda, Tsubasa Yamagata, Jun Nakayama, Masaru Ohme-Takagi
    • Organizer
      第61回日本植物生理学会年会,シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] ELONGATION OF SILIQUIES WITHOUT POLLINATION 3 regulates ovule development in Arabidopsis2020

    • Author(s)
      Hironori Takasaki, Miho Ikeda, Reika Hasegawa, Yillin Zhang, Shingo Sakamoto, Daisuke Maruyama, Nobutaka Mitsuda, Tetsu Kinoshita, Masaru Ohme-Takagi.
    • Organizer
      Aplant Biology 2020 Worldwide Summit
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of EMDOSPERM3 in Arabidopsis2019

    • Author(s)
      Takasaki H, Ikeda M, Zhang Y, Maruyama D, Mitsuda N, Kinoshita T, Ohme-Takagi M
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] シロイヌナズナにおけるENDOSPRM3の機能解析2019

    • Author(s)
      高崎寛則,池田美穂,Yilin Zhang,丸山大輔,光田展隆 木下哲,高木優
    • Organizer
      第60回日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Analysis of ENDOSPERM3, which regulates fertilization-independent endosperm development2019

    • Author(s)
      Yilin Zhang, Hironori Takasaki, Miho Ikeda, Daisuke Maruyama, Nobutaka Mitsuda, Tetsu Kinoshita, Masaru Ohme-Takagi
    • Organizer
      第60回日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 分化全能性を制御する転写因子RSE1 の機能解析2019

    • Author(s)
      中山潤,山形翼,髙崎寛則,池田美穂,高木優
    • Organizer
      第60回日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] RSE1(REPRESSOR OF SOMATIC EMBRYOGENESIS 1) is a transcription factor that regulates cell totipotency in Arabidopsis2018

    • Author(s)
      Miho Ikeda, Tsubasa Yamagata, Masaru Ohme-Takagi
    • Organizer
      The 25th International Congress on Sexual Plant Reproduction
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ENDOSPERM3 is a transcription factor that regulates fertilization-independent endosperm development2018

    • Author(s)
      Hironori Takasaki, Miho Ikeda Nobutaka Mitsuda, Tetsu Kinoshita, Masaru Ohme-Takagi
    • Organizer
      The 25th International Congress on Sexual Plant Reproduction
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of RSE1 (REPRESSOR OF SOMATIC EMBRYOGENESIS 1) transcription factor that control cell totipotency in Arabidopsis2018

    • Author(s)
      Tsubasa Yamagata, Miho Ikeda, Masaru Ohme-Takagi
    • Organizer
      日本植物生理学会 第59回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Analysis of SNB1 transcription factor that positively regulate parthenocarpy2018

    • Author(s)
      Hibari Hayashi, Miho Ikeda, Masaru Ohme-Takagi
    • Organizer
      日本植物生理学会 第59回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 分化全能性を制御する転写因子CR117の機能解析2017

    • Author(s)
      山形翼、池田美穂、高木優
    • Organizer
      日本植物細胞分子生物学会 第35回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] SNB1転写因子による単為結果性誘導メカニズムの解析2017

    • Author(s)
      林陽葉莉、池田美穂、高木優
    • Organizer
      日本植物細胞分子生物学会 第35回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Analysis of SNB1 transcription factor that induces parthenocarpy2017

    • Author(s)
      Hibari Hayashi, Miho Ikeda, Masaru Ohme-Takagi
    • Organizer
      Taiwan-Japan Plant Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of transcription factor that regulates plant cell totipotency in Arabidopsis2017

    • Author(s)
      Tsubasa Yamagata, Miho Ikeda, Masaru Ohme-Takagi
    • Organizer
      Taiwan-Japan Plant Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] 受精無しで胚乳発生を誘導する遺伝子2018

    • Inventor(s)
      高木優、池田美穂、光田展隆
    • Industrial Property Rights Holder
      高木優、池田美穂、光田展隆
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2018 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 受精無しで果実委肥大発生を誘導する遺伝子2018

    • Inventor(s)
      高木優、池田美穂、光田展隆
    • Industrial Property Rights Holder
      高木優、池田美穂、光田展隆
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2018 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 受精を介さず種子植物の胚乳発生を誘導する核酸分子及びベクター、並びに受精を介さず胚乳を発生しうる組換え種子植物及びその作製方法2018

    • Inventor(s)
      高木優、池田美穂、光田展隆、大島良美
    • Industrial Property Rights Holder
      産業技術総合研究所生物 埼玉大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 受精を介さず種子植物の果実の生長を誘導する核酸及びベクター、並びに受精を介さず果実の生長が誘導される組換え種子植物及びその製造方法2018

    • Inventor(s)
      高木優、池田美穂、光田展隆、大島良美
    • Industrial Property Rights Holder
      産業技術総合研究所生物 埼玉大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2017 Annual Research Report

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Published: 2017-07-21   Modified: 2022-01-27  

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