| Budget Amount *help |
¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000)
Fiscal Year 2019: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2018: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2017: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Outline of Final Research Achievements |
In recent years, the development of tissue clearing technology has dramatically advanced the imaging of neural circuit structures by fluorescence microscopy, but the challenge is how to reconstruct neural projections and trace circuits from the acquired images. In this study, we aimed to overcome the following issues: 1) to develop a method to label arbitrary neurons associated with circuit functions, 2) to develop a method for high-density multicolor labeling, and to analyze neural circuits at the resolution of a single cell. Furthermore, 3) we developed a method for extracting neural circuit morphology from multicolor labeled specimens.
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