| Budget Amount *help |
¥24,700,000 (Direct Cost: ¥19,000,000、Indirect Cost: ¥5,700,000)
Fiscal Year 2019: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2018: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2017: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Outline of Final Research Achievements |
A large scale purification system of CAF-I complexes was established and the purified complexes were observed by cryo-EM. The results suggested that the CAF-I complexes disassembled during grid preparation. Subsequently, in order to stabilize the subunit interactions, cross-linking and gel filtration experiments were performed, but the appropriate conditions could not be found. Next, since the dissociation constants of CAF-I and histone H3/H4 are the nanomolar order, the H3/H4 is expected to stabilize CAF-I complex. Then, CAF-I-H3/H4 complexes were purified by gel filtration column. The cryo-EM experiments are in progress, but no clear particle image has been obtained yet.
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