| Project/Area Number |
17H06524
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Tohoku University |
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Principal Investigator |
Naito Takeo 東北大学, 東北メディカル・メガバンク機構, 助教 (80808197)
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| Project Period (FY) |
2017-08-25 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 大腸癌 / 腸管上皮オルガノイド / エクソソーム解析 / エクソソーム / オルガノイド / パネート細胞 / 炎症性腸疾患 |
|---|
| Outline of Final Research Achievements |
In this study, we try to establish small intestinal derived organoid.But cluture was not successful.Therefore, we have produced colon adenoma and cancer-derived organoids easier to culture. Exosomes, are extracellular vesicles havingcontaining a lipid bilayer, and they are known to play an important role in cell-to-cell communication by transmitting exosomal microRNA (miRNA). Therefore, the aim of this study was to clarify Colorectal Cancer characterize colorectal cancer (CRC) -specific exosomal miRNA by using patient-orientedderived organoids. We established a novel exosome extraction method usingTo this end, Among the miRNAs conserved in CRC and colorectal adenoma (CRA) organoids, themiR-1246 expression of miR-1246 was increased in the and exosome.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は大腸癌、腺腫からオルガノイドを作成することに成功し、長期培養することが可能であることを実証した。さらには、培養上清からエクソソームを生成し、大腸癌由来エクソソームにはmir-1246が高発現していることが明らかとした。大腸癌の生存や浸潤に関与していることが推定され、mir-1246の機能が明らかとなれば、治療や診断への応用へ期待できる。
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