Development of an efficient oocyte collection method using active immunization against inhibin in cynomolgus monkeys.
| Project/Area Number |
17H06766
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
Nakaya Masataka 滋賀医科大学, 動物生命科学研究センター, 特任助教 (90805459)
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| Project Period (FY) |
2017-08-25 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 発生工学 / ホルモン / 生殖工学 / 実験動物学 |
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| Outline of Final Research Achievements |
This study examined the induced active immunization against inhibin. The results are summarized as follows: (1) identified a splice variant of inhibin αexpressed in cynomolgus monkey follicles. (2) an amino acid sequence similar or common to “goat” inhibin was identified to induce active immunization. (3) developed a screening system for cynomolgus monkey using serum AMH. (4) generated an Oct3/4 reporter cynomolgus monkey ES Cell line.
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| Academic Significance and Societal Importance of the Research Achievements |
インヒビン免疫中和によるFSH投与への個体間の卵巣応答の均一化、AMH測定による採卵候補個体の選抜による採卵コストの低下、樹立したレポーター発現ES細胞を用いることでカニクイザル発生工学研究の効率化が図られ、本邦の基礎生物学研究や医学研究に大きく貢献することが期待される。また、卵巣のホルモン応答への均一化や採卵候補個体の選抜により、供試動物数を削減できることから、本研究の成果は実験動物福祉の観点からも非常に重要であると考えられる。
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Report
(3 results)
Research Products
(2 results)
