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Development of an efficient oocyte collection method using active immunization against inhibin in cynomolgus monkeys.

Research Project

Project/Area Number 17H06766
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionShiga University of Medical Science

Principal Investigator

Nakaya Masataka  滋賀医科大学, 動物生命科学研究センター, 特任助教 (90805459)

Project Period (FY) 2017-08-25 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords発生工学 / ホルモン / 生殖工学 / 実験動物学
Outline of Final Research Achievements

This study examined the induced active immunization against inhibin. The results are summarized as follows: (1) identified a splice variant of inhibin αexpressed in cynomolgus monkey follicles. (2) an amino acid sequence similar or common to “goat” inhibin was identified to induce active immunization. (3) developed a screening system for cynomolgus monkey using serum AMH. (4) generated an Oct3/4 reporter cynomolgus monkey ES Cell line.

Academic Significance and Societal Importance of the Research Achievements

インヒビン免疫中和によるFSH投与への個体間の卵巣応答の均一化、AMH測定による採卵候補個体の選抜による採卵コストの低下、樹立したレポーター発現ES細胞を用いることでカニクイザル発生工学研究の効率化が図られ、本邦の基礎生物学研究や医学研究に大きく貢献することが期待される。また、卵巣のホルモン応答への均一化や採卵候補個体の選抜により、供試動物数を削減できることから、本研究の成果は実験動物福祉の観点からも非常に重要であると考えられる。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • Research Products

    (2 results)

All 2019

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results)

  • [Journal Article] Generation of an OCT3/4 reporter cynomolgus monkey ES cell line using CRISPR/Cas9.2019

    • Author(s)
      Kobayashi K, Tsukiyama T, Nakaya M, Kageyama S, Tomita K, Murai R, Yoshida T, Narita M, Kawauchi A, Ema M.
    • Journal Title

      Stem cell research

      Volume: Epub Pages: 101439-101439

    • DOI

      10.1016/j.scr.2019.101439

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comprehensive evaluation of ubiquitous promoters suitable for the generation of transgenic cynomolgus monkeys†2019

    • Author(s)
      Seita Yasunari、Tsukiyama Tomoyuki、Azami Takuya、Kobayashi Kenichi、Iwatani Chizuru、Tsuchiya Hideaki、Nakaya Masataka、Tanabe Hideyuki、Hitoshi Seiji、Miyoshi Hiroyuki、Nakamura Shinichiro、Kawauchi Akihiro、Ema Masatsugu
    • Journal Title

      Biology of Reproduction

      Volume: 印刷中 Issue: 6 Pages: 1440-1452

    • DOI

      10.1093/biolre/ioz040

    • NAID

      120006594188

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research

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Published: 2017-08-25   Modified: 2020-03-30  

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