| Project/Area Number |
17H06799
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Integrative animal science
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| Research Institution | Kyoto University |
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Principal Investigator |
Sandeep Goel 京都大学, 農学研究科, 特定准教授 (80801745)
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| Research Collaborator |
KOIDE Tsuyoshi National Institute of Genetics, Mouse Genome Resource Laboratory, Professor
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| Project Period (FY) |
2017-08-25 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | testis / spermatogenesis / protein kinase / gene knockout / Testis / Spermatogenesis / Protein Kinase / Gene knockout / Infertility / Protein kinase / Null mutants / Male infertility / Genome editing |
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| Outline of Final Research Achievements |
PBK expression is exclusively detected in the testis of adult males. However, its role in testis remains elusive. To investigate, PBK knockout (KO) mice were generated. Parameters such as testis, epididymis, seminal vesicle weights, tubular diameter, litter size, epididymal sperm number, percentage of viable and morphologically normal sperm did not differ between the KO and wild type (WT) mice (P > 0.05). Fertilization competence of sperm of KO mice and oocyte development competence post-fertilization was comparable to WT mice. Protein expression analysis showed that P-P38 expression was elevated in KO mice as compared to WT mice (P < 0.05). However, expression of apoptosis, cell proliferation, spermatogonia, spermatogonial stem cell and meiotic cell -specific proteins were comparable between KO and WT mice testis (P > 0.05). In conclusion, deletion of PBK gene has no effect on the fertility of male mice possibly due to compensation of loss of PBK by some other kinase in the testis.
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| Academic Significance and Societal Importance of the Research Achievements |
PBK is a cancer-testis antigen expressed exclusively in adult testis and in highly metastatic tumors. Since its role in testis remains unknown, we hypothesized that PBK may have a critical role in male fertility. Results show that PBK is dispensable for fertility in males.
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