The mechanism for the proliferation of epidermal stem cells during pregnancy

• Project/Area Number: 17H06809
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Kyoto University
• Principal Investigator: Ichijo Ryo 京都大学, ウイルス・再生医科学研究所, 特定助教 (50804382)
• Project Period (FY): 2017-08-25 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 妊娠 / 表皮 / 幹細胞 / 皮膚 / 表皮幹細胞

Outline of Final Research Achievements

In this study, we conducted the research to reveal the heterogeneity of interfollicular epidermal stem cells in pregnancy. Pregnancy-specific cell populations were identified by single cell RNA sequence using Tbx3-creERT2 / R26-H2B-EGFP mice. By extracting EGFP positive cells on the data, it revealed that the gene expression of EGFP positive cells differentiated. We also confirmed that EGFP positive cells were delaminated after delivery by whole mount staining.

Academic Significance and Societal Importance of the Research Achievements

近年、表皮幹細胞は均一な細胞群であると考えられている。よって、本研究により、表皮幹細胞のheterogeneityが証明されれば、現状では解明が困難であった皮膚病や癌研究に貢献できる可能性がある。表皮幹細胞のheterogeneityが基底細胞の増殖を亢進するメカニズムを解明することは、新たな表皮の増殖制御機構を明らかにする可能性があり、妊娠や老化などの「ライフステージ」による皮膚変化の解明に応用でき、社会に与えるインパクトは大きいことが予想される。

Research Products

• [Journal Article] Transient elevation of cytoplasmic calcium ion concentration at a single cell level precedes morphological changes of epidermal keratinocytes during cornification (2018)
  • Author(s): Murata Teruasa、Honda Tetsuya、Egawa Gyohei、Yamamoto Yasuo、Ichijo Ryo、Toyoshima Fumiko、Dainichi Teruki、Kabashima Kenji
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 1-10
  • DOI: 10.1038/s41598-018-24899-7
  • NAID: 120006522872
  • Peer Reviewed / Open Access
• [Journal Article] Tbx3-dependent amplifying stem cell progeny drives interfollicular epidermal expansion during pregnancy and regeneration (2017)
  • Author(s): Ichijo, R., Kobayashi, H., Yoneda, S., Iizuka, Y., Kubo, H., Matsumura, S., Kitano, S., Miyachi, H., Honda, T., and Toyoshima, F.
  • Journal Title: Nat. Commun. Volume: 8 Issue: 1 Pages: 508-508
  • DOI: 10.1038/s41467-017-00433-7
  • NAID: 120006346261
  • Peer Reviewed / Open Access
• [Presentation] 高増殖能を有する表皮幹細胞のダイナミクス (2018)
  • Author(s): 一條 遼
  • Organizer: 第41回日本分子生物学会年会
• [Presentation] 妊娠期の腹側皮膚伸展を可能とする表皮幹細胞ダイナミクスの解明 (2017)
  • Author(s): 一條 遼、小林 大毅、米田 早織、飯塚 ゆい、久保 嘉一、松村 繁、北野 さつき、宮地 均、本田 哲也、豊島 文子
  • Organizer: 2017年度生命科学系学会合同年次大会