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Nano / Bottom-Up / Fabrication of TaylorMade 3D Bone Tissue for Clinical Application

Research Project

Project/Area Number 17H07018
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Dental engineering/Regenerative dentistry
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

Yoshiki Sato  京都府立医科大学, 医学(系)研究科(研究院), 特任助教 (50808235)

Project Period (FY) 2017-08-25 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsスキャホールド / ナノゲル / ダイレクト・リプログラミング / 骨芽細胞 / 再生医療 / 多孔性架橋ハイブリッドナノゲル / 再生医学 / 免疫学
Outline of Final Research Achievements

To treat patients with bone diseases including alveolar bone resorption by means of bone regenerative therapy, it should be important to devise a novel scaffold that allow efficient adhesion of and bone matrix production by osteoblasts. Here we established a novel porous cross-linked nanogel by means of the nanogel techtonics. This scaffold supported adhesion and proliferation of induced osteoblasts, as well as regeneration of injured bone tissue in vivo. This porous hybrid nanogel is adequate for transplantation therapy, due to its safety and functions.

Academic Significance and Societal Importance of the Research Achievements

多孔性架橋ハイブリッド・ナノゲルは、安全な自家骨細胞移植の足がかりとして大きな一歩となる。フリーズドライ処理を行うことで、3次元培養の精度が上がり、長期保存も可能となることから臨床応用可能な技術に近づいたといえる。本研究で多孔性架橋ハイブリッド・ナノゲルとダイレクト・リプログラミングによる大量の骨芽細胞を接着させ、移植細胞の創部からの漏洩の問題を解決し、一塊として簡易な術式で組織移植が行えたことは臨床応用への術式簡素化という意味で意義がある。動物実験では、マイクロCTおよび組織切片にて移植部に機能的な骨再生が確認でき、臨床応用に向けた基礎データが得られた。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • Research Products

    (8 results)

All 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Nanogel tectonic porous 3D scaffold for direct reprogramming fibroblasts into osteoblasts and bone regeneration.2018

    • Author(s)
      Sato Y, Yamamoto K, Horiguchi S, Tahara Y, Nakai K, Kotani SI, Oseko F, Pezzotti G, Yamamoto T, Kishida T, Kanamura N, Akiyoshi K, Mazda O.
    • Journal Title

      Sci Rep

      Volume: 8 Issue: 1 Pages: 15824-15824

    • DOI

      10.1038/s41598-018-33892-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Direct phenotypic conversion of human fibroblasts into functional osteoblasts triggered by a blockade of the transforming growth factor-β signal.2018

    • Author(s)
      Yamamoto K, Kishida T, Nakai K, Sato Y, Kotani SI, Nishizawa , Yamamoto T, Kanamura N, Mazda O.
    • Journal Title

      Sci Rep

      Volume: 8 Issue: 1 Pages: 8463-8463

    • DOI

      10.1038/s41598-018-26745-2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Osteoblast transplantation treatment of large scale bone defect using direct reprogramming technique and 3D scaffold.2018

    • Author(s)
      Sato Y, Yamamoto K, Nakai K, Oseko F, Hasegawa A, Kishida T, Mazda O, Yamamoto T, Kanamura N
    • Organizer
      13th Asian Congress on Oral & Maxillofacial Surgery (ACOMS)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ダイレクト・コンバージョン技術を用いた新規骨再生療法の開発2018

    • Author(s)
      中井 敬, 佐藤良樹, 山本健太,足立哲也,足立圭司,大迫文重,雨宮 傑,山本俊郎,金村成智.
    • Organizer
      第61回秋季日本歯周病学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Establishment of bone regeneration therapy by direct conversion using rat fibroblasts.2018

    • Author(s)
      Sato Y, Yamamoto K, Nakai K, Oseko F, Kishida T, Mazda O, Yamamoto T, Kanamura N.
    • Organizer
      40th Asia Pacific Dental Congress (APDC)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ダイレクト・リプログラミング技術を用いた新規骨再生療法の開発.2018

    • Author(s)
      佐藤良樹, 山本健太, 中井敬, 堀口智史, 足立哲也, 足立圭司, 大迫文重, 雨宮傑, 坂下敦宏, 山本俊郎, 金村成智.
    • Organizer
      第147回日本歯科保存学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ダイレクト・リプログラミング技術を用いた新規骨再生療法の開発2017

    • Author(s)
      佐藤良樹, 山本健太, 中井敬, 堀口智史, 足立哲也, 足立圭司, 大迫文重, 雨宮傑, 坂下敦宏, 山本俊郎, 金村成智.
    • Organizer
      第147回日本歯科保存学会
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 多孔質三次元細胞培養足場材料及びその製造方法2018

    • Inventor(s)
      佐藤良樹 山本健太 堀口智史 山本俊郎 岸田綱郎 松田修
    • Industrial Property Rights Holder
      京都府公立大学法人
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Acquisition Date
      2018
    • Related Report
      2018 Annual Research Report

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Published: 2017-08-25   Modified: 2020-03-30  

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