| Project/Area Number |
17H07105
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Hygiene and public health
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| Research Institution | Showa University |
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Principal Investigator |
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| Research Collaborator |
Yamano Yuko
Ichihara Gaku
Ichihara Sahoko
Sakurai Toshihiro
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| Project Period (FY) |
2017-08-25 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | アクリルアミド / 神経毒性 / プロテオーム解析 / プロテオミクス |
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| Outline of Final Research Achievements |
In this study, we attempted to elucidate the mechanism of acrylamide-induced neurotoxicity and to search for a therapeutic effect for neurotoxicity by vitamin B1. The expression of five proteins confirmed by Western blotting was performed in PC12 cells. PC12 cells were elongated by nerve growth factor and acrylamide was exposed at concentrations from 0mM to 10mM. As a result of this study, there were no significant changes in the expression of five proteins, unlike the animal experiments. However, concentration of intracellular ATP was significantly reduced at 30 minutes after exposure. We administered vitamin B1 after exposure to acrylamide, there was no significant recovery thorough the amount of ATP.
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| Academic Significance and Societal Importance of the Research Achievements |
acrylamideは疫学研究において、長期曝露により感覚障害や歩行障害といいた末梢神経のみならず、記憶障害や言語障害、抑うつ症状といった中枢神経障害を引き起こすことが知られているが、その毒性発現メカニズムは十分に解明されていない。本研究結果では、acrylamide曝露時の影響マーカーを探索し、エネルギー産生量について検討することで、神経毒性発現メカニズムの一端を明らかにした。
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