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Induction of apoptosis by TGF-beta isoforms in amelogenesis

Research Project

Project/Area Number 17H07198
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionTsurumi University

Principal Investigator

Kobayashi Saeko  鶴見大学, 歯学部, 助教 (90804534)

Project Period (FY) 2017-08-25 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsTGF-β / エナメル質 / エナメルタンパク質 / アポトーシス / エナメル芽細胞 / 生理活性物質 / エナメルタンパク / 歯学 / 細胞・組織 / 再生医学 / シグナル伝達
Outline of Final Research Achievements

Objectives are to elucidate it about how role of TGF-β isoforms during enamel formation. We were using a dental epithelial cell line derived from the apical bud of a mouse incisor (mHAT9d cells). We prepared mRNAs from culture supernatant of mHAT9d for Quantitative PCR Analysis of three TGF-β isoforms and several enamel proteins. TGF-β isoforms significantly upregulated the mRNA level of gene expression of related genes for maturation-stage ameloblasts in mHAT9d cells. Furthermore, the total number of caspase-3-positive apoptotic events and amount of ssDNA were significantly rose and there was the homogeny about their distribution in three TGF-β isoforms. TGF-β is regulated in an isoform-specific manner to exert multiple biological functions such as cell proliferation, gene expression and apoptosis in maturation-stage ameloblasts.

Academic Significance and Societal Importance of the Research Achievements

本研究ではエナメル質形成過程におけるTGF-βの役割について追究を行った。TGF-βがアポトーシス誘導能を有する可能性を示唆するだけでなく、アイソフォーム特異的な発現の時期や分布があること、さらにそれぞれに違う役割を有している可能性を見出した。生理活性物質に注目した研究は、正常なエナメル質の形成メカニズムを知ることのみならず、歯周組織やエナメル質の再生医療への大きな足掛かりになると期待している。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • Research Products

    (5 results)

All 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results)

  • [Int'l Joint Research] The Forsyth Institute(米国)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Potential Function of TGF-b Isoforms in Maturation-Stage Ameloblasts2019

    • Author(s)
      Okubo M, Chiba R, Karakida T, Yamazaki H, Yamamoto R, Kobayashi S, Niwa T, Margolis HC, Nagano T, Yamakoshi Y and Gomi K
    • Journal Title

      J Oral Biosci.

      Volume: 61(1) Issue: 1 Pages: 43-54

    • DOI

      10.1016/j.job.2018.12.002

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] TGF-β autocrine signaling at secretory-stage enamel.2018

    • Author(s)
      Kobayashi S, Yamakoshi Y, and Asada Y.
    • Journal Title

      J Oral Biosci.

      Volume: 60, (3) Issue: 3 Pages: 70-75

    • DOI

      10.1016/j.job.2018.05.001

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] TGF-β signaling during amelogenesis (エナメル質形成過程におけるTGF-βのシグナル伝達)2018

    • Author(s)
      大久保水羽、千葉理沙子、唐木田丈夫、山本竜司、小林冴子、長野孝俊、五味一博、山越康雄
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Induction of apoptosis by TGF-β isoforms in amelogenesis(エナメル質形成過程における TGF-βによるアポトーシス誘導について)2018

    • Author(s)
      小林冴子、山越康雄、朝田芳信
    • Organizer
      第56回日本小児歯科学会大会
    • Related Report
      2018 Annual Research Report

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Published: 2017-08-25   Modified: 2020-03-30  

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