Development of iPS cell-based functional working memory model in vitro
| Project/Area Number |
17H07358
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Yada Yuichiro 国立研究開発法人理化学研究所, バイオリソース研究センター, 特別研究員 (80805797)
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| Research Collaborator |
Inoue Haruhisa
Imamura Keiko
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| Project Period (FY) |
2017-08-25 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 多点電極アレイ / iPS細胞由来神経細胞 / 神経同期活動 / 神経工学 / ワーキングメモリ |
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| Outline of Final Research Achievements |
The aim of this project was developing a method to evaluate cellular working memory by electrical stimulation. We cultivated mouse/human iPSC (induced pluripotent stem cell) derived neurons on multi-electrode array, so that patterned stimulation can be added to the cell population. We investigated the suitable experimental conditions, including cultivation period, of the cells for the experiment.
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| Academic Significance and Societal Importance of the Research Achievements |
iPS細胞を使用して培養皿上に神経疾患を再現し、有効な薬剤を探索するなどの創薬基盤として利用することに注目が集まっている。認知症などの神経「機能」の疾患モデルを評価する上では、遺伝子やタンパク質の発現、細胞死や形態変化に留まらず、培養皿上の神経集団機能を評価する方法の開発が有用と考えられる。本研究を礎として、電気刺激応答による機能評価手法の開発が期待される。
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Report
(3 results)
Research Products
(1 results)
