• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of the resolution mechanism of inflammation via central carbon metabolism and 12/15-lipoxygenase in eosinophil

Research Project

Project/Area Number 17H07368
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Okahashi Nobuyuki  国立研究開発法人理化学研究所, 生命医科学研究センター, 客員研究員 (30802748)

Project Period (FY) 2017-08-25 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords12/15-リポキシゲナーゼ / リピッドメディエーター / 好酸球 / レドックスバランス / 中心炭素代謝 / リポキシゲナーゼ / 抗炎症メディエーター
Outline of Final Research Achievements

Previous studies demonstrated that the activity of 12/15 lipoxygenase (LOX), an oxidation enzyme of polyunsaturated fatty acids, expressed in eosinophil is related to the production of anti-inflammatory lipid mediators and resolution of inflammation. However, the regulation mechanism of 12/15-LOX activity is not well understood. We hypothesized that the rapid neutralization of the lipid peroxide produced via 12/15-LOX by reducing cofactors could be essential to keep higher enzymatic activity of 12/15-LOX. In this project, the role of metabolism in eosinophil was explored by the quantitative measurement of lipid mediators and metabolites in central carbon metabolism using mass spectrometry。

Academic Significance and Societal Importance of the Research Achievements

本研究の学術的意義は、脂質代謝が基質量や酵素量だけでなく、還元力という側面からも制御されうることを示唆した点である。この結果は、アレルギー、癌やアルツハイマー病など炎症反応が病態に関与する多くの疾患に対して、還元力が新たな治療法開発の糸口となる可能性を示している。また、還元力に注目して生体内の抗炎症脂質メディエーターの産生量を高めることができれば、ω3脂肪酸の特定保健用食品の効果を最大化し、医療用医薬品に頼らない疾患予防の発展にもつながる点が本研究の社会的意義である。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • Research Products

    (4 results)

All 2019 2018

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (1 results) Book (1 results)

  • [Journal Article] 糖リン酸の精密計測が明らかにする中心代謝フラックス2019

    • Author(s)
      岡橋伸幸, 松田史生
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 77 Pages: 116-118

    • NAID

      40021840502

    • Related Report
      2018 Annual Research Report
    • Open Access
  • [Journal Article] Sugar phosphate analysis with base line separation and soft ionization by gas chromatography-negative chemical ionization-mass spectrometry improves flux estimation of bidirectional reactions in cancer cells.2018

    • Author(s)
      Nobuyuki Okahashi, Kousuke Maeda, Shuichi Kawana, Junko Iida, Hiroshi Shimizu, Fumio Matsuda
    • Journal Title

      Metabolic Engineering

      Volume: 51 Pages: 43-49

    • DOI

      10.1016/j.ymben.2018.08.011

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] ガスクロマトグラフィー負化学イオン化質量分析法を用いた糖リン酸新規分析法の構築と13C代謝フラックス解析への応用2018

    • Author(s)
      岡橋伸幸, 前田昂亮, 川名修一, 飯田順子, 清水浩, 松田史生
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Annual Research Report
  • [Book] 代謝センシング -健康、食、美容、薬、そして脳の代謝を知る-2018

    • Author(s)
      岡橋伸幸, 松田史生, 清水浩
    • Total Pages
      249
    • Publisher
      シーエムシー出版
    • ISBN
      9784781313504
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2017-08-25   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi