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Study of glioma infiltration in complex microenvironment on an automated microfluidic chip

Research Project

Project/Area Number 17J00362
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionOkinawa Institute of Science and Technology Graduate University

Principal Investigator

TSAI Hsieh Fu  沖縄科学技術大学院大学, 科学技術研究科, 特別研究員(DC1)

Project Period (FY) 2017-04-26 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2019: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2018: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2017: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordshybrid microfluidics / glioblastoma / voltage-gated ionchannel / deep learning / neural network / cell migration / cell tracking / Hybrid / label-free / machine learning / single-cell segmentation / perivasculature / neuron culture / label-free segmentation / electrotaxis / temperature controller
Outline of Annual Research Achievements

A. A two-layer hybrid microfluidic device is developed for high throughput electrotaxis study. A robust hybrid microfluidic device composed of PMMA/PDMS is developed with 2-layer design for high throughput electrotaxis study of glioblastoma. A method to uniformly seed sparse cells in microchannel is also achieved by balancing inlet/outlet pressure.
B. Electrotaxis of glioblastoma requires optimal laminin-containing extracellular matrix. Using the platform and the label-free cell tracking software we developed before, T98G and U-251MG glioblastoma electrotaxis are investigated and been found to depend on laminin extracellular matrix
C. Electrotaxis of glioblastoma cell models can be heterogenous. We have also found that T98G and U-251MG despite being molecularly and phenotypically typed similarly as mesenchymal glioblastoma, the electrotactic behavior is completely different. The result may reflect the heterogeneity of the brain cancer.
D. Electrotaxis of glioblastoma cells are mediated by voltage-gated ion channels. The calcium signaling plays a pivotal role in cell electrotaxis as well as various aspects of glioblastoma biology such as drug resistance and proliferation. We investigated if the voltage-gated calcium channels (VGCC) mediate the electrotaxis of glioblastoma and that different VGCCs are involved in different glioblastoma cell lines. Other ion channels such as voltage-gated potassium channels and the amiloride-sensitive sodium channels also mediates the glioblastoma cell electrotaxis. showing the importance of confirming patient-specific ion expression profile.

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (5 results)

All 2020 2019 2017 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Glioblastoma adhesion in a quick-fit hybrid microdevice2019

    • Author(s)
      Hsieh-Fu Tsai, Kazumi Toda-Peters, Amy Q. Shen
    • Journal Title

      Biomedical Microdevices

      Volume: 21 Issue: 2 Pages: 1-14

    • DOI

      10.1007/s10544-019-0382-0

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Usiigaci: Instance-aware cell tracking in stain-free phase contrast microscopy enabled by machine learning2019

    • Author(s)
      Hsieh-Fu Tsai, Joanna Gajda, Tyler F.W. Sloan, Andrei Rares, Amy Q. Shen
    • Journal Title

      SoftwareX

      Volume: 9 Pages: 230-237

    • DOI

      10.1016/j.softx.2019.02.007

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tumour-on-a-chip: microfluidic models of tumour morphology, growth and microenvironment2017

    • Author(s)
      Tsai Hsieh-Fu、Trubelja Alen、Shen Amy Q.、Bao Gang
    • Journal Title

      Journal of Royal Society Interface

      Volume: 14 Issue: 131 Pages: 1-20

    • DOI

      10.1098/rsif.2017.0137

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Remarks] Usiigaci opensource repository

    • URL

      https://github.com/oist/Usiigaci

    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] A DETACHABLE MICROFLUIDIC SYSTEM AND METHOD FOR ROBUST SPARSE CELL SEEDING WITHOUT BUBBLES.2020

    • Inventor(s)
      HSIEH-FU TSAI, AMY Q. SHEN
    • Industrial Property Rights Holder
      HSIEH-FU TSAI, AMY Q. SHEN
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2019 Annual Research Report
    • Overseas

URL: 

Published: 2017-05-25   Modified: 2024-03-26  

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