| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Pyruvic oxime dioxygenase (POD) is a key enzyme participating in a heterotrophic nitrification. Using designed specific primers, we attempted PCR amplification of the POD gene using environmental DNA collected from various kinds of soil. All the products obtained were POD genes from Beauveria bassiana, a eukaryotic microorganism of the phylum Ascomycota. Further we conducted biochemical analysis of the recombinant of B. bassiana POD, and the cultivation experiments using the isolate of B. bassiana. Those results indicate that B. bassiana may be significantly involved in heterotrophic nitrification which is highly active especially in the acidic forest soils.
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