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Elucidation of roles of hydration for inducing DNA strand breakage by ionizing radiations

Research Project

Project/Area Number 17K00564
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Risk sciences of radiation and chemicals
Research InstitutionNational Institutes for Quantum and Radiological Science and Technology

Principal Investigator

FUJII KENTARO  国立研究開発法人量子科学技術研究開発機構, 次世代放射光施設整備開発センター, 上席研究員(定常) (00360404)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords生体分子 / プロトン移動 / 軟X線 / 分子変化 / アデノシン / 電荷移動 / 水和 / デオキシリボース / 中性種の脱離 / DNA主鎖切断 / 水和水分子 / 重水素置換 / 脱離イオン / DNA鎖切断 / 放射線
Outline of Final Research Achievements

Alterations resulting proton or electron transfer between biomolecules such as DNA nucleobases and surrounding water molecules were investigated. Adenosine mono-nucleotide (AMP) and deoxy adenosine mono-nucleotide (dAMP) were chosen as target molecules. In order to investigate the alterations, X-ray absorption near edge structures (XANES) were observed before and after soft X-ray irradiations for the solid films of the target samples in a vacuum chamber. The obtained results showed that sugar site is one of the most fragile site in the nucleotide molecule. It is known that 4' position is one of the most fragile position in a pentose ring. Proton transfer from surrounding water molecules to 4'-position of pentose ring might induce degradation of sugar site of nucleotide molecules.

Academic Significance and Societal Importance of the Research Achievements

光合成などの生体分子内のエネルギー産生能力は非常に高効率で、そのメカニズムを利用したエネルギー生成が実現できれば、省エネルギーデバイスなどの実現に向けて大きく前進することが期待される。また核酸塩基と水分子は自然環境に当たり前に存在する物質であり、これらを利用したデバイスの応用は、環境適合材料としても最も適したものの一つと考えられ、生産から廃棄に至るまでのエネルギーコストは極めて少なく抑えられる。イオン液体内の電荷移動現象の基礎過程に関する知見となり、技術的な革命が期待されている生体分子を用いた電子デバイスの開発において重要な知見となることが期待される。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (48 results)

All 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (10 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 10 results,  Open Access: 7 results) Presentation (37 results) (of which Int'l Joint Research: 17 results,  Invited: 4 results)

  • [Int'l Joint Research] Piere Marie Curie University/Essonne University(フランス)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Electronic properties of DNA-related molecules containing a bromine atom2020

    • Author(s)
      Hirato Misaki、Onizawa Misato、Baba Yuji、Haga Yoshinori、Fujii Kentaro、Wada Shin-ichi、Yokoya Akinari
    • Journal Title

      International Journal of Radiation Biology

      Volume: - Issue: 1 Pages: 1-7

    • DOI

      10.1080/09553002.2020.1800121

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hydration of Nucleobase as Probed by Electron Emission of Uridine-5’-Mono-Phosphate (UMP) in Aqueous Solution Induced by Nitrogen K-Shell Ionization2020

    • Author(s)
      Yasuaki Takeda, Hiroyuki Shimada, Ryosuke Miura, Masatoshi Ukai, Kentaro Fujii, Yoshihiro Fukuda, Yuji Saitoh
    • Journal Title

      Quantum Beam Science

      Volume: 4 Issue: 1 Pages: 10-10

    • DOI

      10.3390/qubs4010010

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 放射線によるアデノシン三リン酸分子変異によって誘発される生物学的効果2020

    • Author(s)
      藤井 健太郎, 加藤 大, 藤井 紳一郎, 月本 光俊, 秋光 信佳
    • Journal Title

      X線分析の進歩

      Volume: 51 Pages: 251-259

    • NAID

      40022215594

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ab Initio Molecular Dynamics Simulations to Interpret the Molecular Fragmentation Induced in Deoxyribose by Synchrotron Soft X-Rays2019

    • Author(s)
      Marie-Anne Herve du Penhoat, Anis Hamila, Marie-Pierre Gaigeot, Rodolphe Vuilleumier , Kentaro Fujii, Akinari Yokoya
    • Journal Title

      Quanum Beam Science

      Volume: 3 Issue: 4 Pages: 24-24

    • DOI

      10.3390/qubs3040024

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] NOVEL ANALYTICAL STUDY FOR REACTION INTERMEDIATES IN THE PRIMARY RADIATION INTERACTION OF DNA USING A SYNCHROTRON RADIATION-INDUCED LUMINESCENCE SPECTROSCOPY2019

    • Author(s)
      Kojima T、Aihara H、Kodashima Y、Makishima H、Nakiri S、Takada S、Shimada H、Ukai M、Ozga C、Holzapfel X、Schmidt Ph、Kostner-Wetekam C、Otto H、Bloβ D、Knie A、Ehresmann A、Yokoya A、Fujii K、Fukuda Y、Saitoh Y
    • Journal Title

      Radiation Protection Dosimetry

      Volume: - Issue: 1-2 Pages: 32-35

    • DOI

      10.1093/rpd/ncy239

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] XANES spectral changes of hydrated deoxyribose induced by K-shell ionization of oxygen2019

    • Author(s)
      Fujii Kentaro、Yokoya Akinari
    • Journal Title

      AIP Conference Proceedings

      Volume: 2054 Pages: 040005-040005

    • DOI

      10.1063/1.5084606

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Substituent effect on the yield of unpaired electrons in DNA bases studied by electron paramagnetic resonance2018

    • Author(s)
      Oka Toshitaka、Yokoya Akinari、Fujii Kentaro、Kino Yasushi、Sekine Tsutomu
    • Journal Title

      Applied Physics Letters

      Volume: 113 Issue: 24 Pages: 243701-243701

    • DOI

      10.1063/1.5055722

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Roles of Hydration for Inducing Decomposition of 2-deoxy-D-ribose by Ionization of Oxygen K-Shell Electrons2018

    • Author(s)
      Kentaro Fujii, Yudai Izumi, Ayumi Narita, Krishina Ghose, Pable Lopez-Tarifa, Alain Touati, Riccardo Spezia, Rodolphe Vuilleumier, Marie-Pierre Gaigeot, Marie-Francoise Politis, Marie-Anne Herve du Penhoat, Akinari Yokoya
    • Journal Title

      Radiation Research

      Volume: 189 Issue: 3 Pages: 264-272

    • DOI

      10.1667/rr14225.1

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A significant role of non-thermal equilibrated electrons in the formation of deleterious complex DNA damage2018

    • Author(s)
      Takeshi Kai, Akinari Yokoya, Masatoshi Ukai, Kentaro Fujii, Tomohiro Toigawa, Rituko Watanabe
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 92 Issue: 4 Pages: 660-664

    • DOI

      10.1039/c7cp06903k

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Circular dichroism spectroscopic study on structural alterations of histones induced by post-translational modifications in DNA damage responses: Lysine-9 methylation of H32017

    • Author(s)
      Yudai Izumi, Koichi Mastuo, Kentaro Fujii, Akinari Yokoya, Masaki Taniguchi, Hirofumi Namatame
    • Journal Title

      Journal of Radiation Research

      Volume: 59 Issue: 2 Pages: 108-115

    • DOI

      10.1093/jrr/rrx068

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 軟X線光電子分光法を用いた臭素化DNA関連分子の電子状態の研究2021

    • Author(s)
      平戸 未彩紀, 馬場 祐治, 和田 真一, 藤井 健太郎, 横谷 明徳
    • Organizer
      第34回日本放射光学会年会・放射光科学合同シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] 次世代放射光施設 共用ビームライン検討状況 -軟X線ナノ吸収分光(XMCD)ビームライン-2021

    • Author(s)
      今園 孝志, 上野 哲朗, 岩澤 英明, 大坪 嘉之, 堀場 弘司, 宮脇 淳, 中谷 健, 藤井 健太郎, 安居院 あかね, 木村 洋昭, 高橋 正光
    • Organizer
      第34回日本放射光学会年会・放射光科学合同シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] 放射線生物研究と軟X線分光2021

    • Author(s)
      藤井 健太郎
    • Organizer
      第34回日本放射光学会年会 放射光科学合同シンポジウム
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 次世代放射光施設 共用ビームライン検討状況 -軟X線超高分解能共鳴非弾性散乱(RIXS)ビームライン-2021

    • Author(s)
      宮脇 淳, 藤井 健太郎, 今園 孝志, 堀場 弘司, 大坪 嘉之, 中谷 健, 安居院 あかね, 木村 洋昭, 高橋 正光
    • Organizer
      第34回日本放射光学会年会 放射光科学合同シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] 次世代放射光施設 共用ビームライン検討状況 -軟X線ナノ光電子分光(ARPES)ビームライン-2021

    • Author(s)
      堀場 弘司, 今園 孝志, 岩澤 英明, 藤井 健太郎, 宮脇 淳, 大坪 嘉之, 中谷 健, 安居院 あかね, 木村 洋昭, 高橋 正光
    • Organizer
      第34回日本放射光学会年会・放射光科学合同シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] 細胞の放射線増感を誘発する臭素化DNA関連分子の電子状態の研究2020

    • Author(s)
      平戸 未彩紀, 馬場 祐治, 藤井 健太郎, 和田 真一, 黒川 悠索, 中辻 博, 横谷 明徳
    • Organizer
      量子生命科学会第2回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 次世代放射光施設を利用した量子生命科学研究の展開2020

    • Author(s)
      藤井 健太郎
    • Organizer
      量子生命科学先端フォーラム
    • Related Report
      2020 Annual Research Report
  • [Presentation] 次世代放射光を利用した量子生命科学研究の展望2020

    • Author(s)
      藤井 健太郎, 平野 優, 玉田 太郎, 宮脇 淳, 泉 雄大, 原田 慈久, 安達 基泰
    • Organizer
      量子生命科学会 第2回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Physical Process of Radiation Damage to DNA utilizing Soft X-ray Spectroscopy2020

    • Author(s)
      Kentaro Fujii, Akinari Yokoya, M-A. Herve du Penhoat, M-F. Politis
    • Organizer
      The international workshop for synergetic collaboration between material and synchrotron science through IMR-MAX IV joint research
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Physical Process of DNA Strand Breakages by Ionizing Radiations utilizing Synchrotron Soft X-ray Spectroscopy2020

    • Author(s)
      Kentaro Fujii, Akinari Yokoya, M-A. Herve du Penhoat, M-F. Politis
    • Organizer
      The 8th International Symposium of Gunma University Initiative for Advanced Research
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Physical Process of DNA Strand Breakage induced by Core-Electron Ionization2019

    • Author(s)
      Kentaro Fujii, 藤井 健太郎, Akinari Yokoya, 横谷 明徳, Marie-Anne Herve du Penhoat, Marie-Francoise Politis
    • Organizer
      International Young Researchers Workshop on Synchrotron Radiation Science
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 軟X線を用いたDNA損傷生成過程の研究2019

    • Author(s)
      藤井 健太郎
    • Organizer
      第25回HiSOR研究会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Physical Process of DNA Strand Breakage induced by Ionizing Radiations2019

    • Author(s)
      Kentaro Fujii, Akinari Yokoya, Marie-Anne Herve du Penhoat, Marie-Francoise Politis
    • Organizer
      International Conference on Materials and System for Sustainability
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 放射光軟X線分光を用いた電離放射線誘発DNA主鎖切断の物理過程の観測2019

    • Author(s)
      藤井 健太郎, 横谷 明徳, Marie-Anne Herve du Penhoat, Marie-Francoise Politis
    • Organizer
      量子生命科学会 第1回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Fragmentation of Doubly-Ionized Deoxyribose2019

    • Author(s)
      M-A. Herve du Penhoat, A. Souchaud, R. Vuilleumier, M-P. Gaigeot, Kentaro Fujii, Akinari Yokoya, M-F. Politis
    • Organizer
      International Congress of Radiation Research 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] XANESスペクトルによる放射線DNA鎖切断の観測2019

    • Author(s)
      藤井健太郎, 横谷明徳, M-A.Herve du Penhoat, M-F.Politis
    • Organizer
      第32回日本放射光学会年会・放射光科学合同シンポジウム
    • Related Report
      2018 Research-status Report
  • [Presentation] Soft X-ray spectroscopies for Br-incorporated DNA nucleotide2019

    • Author(s)
      Misaki Hirato, Kentaro Fujii, Akinari Yokoya, Shin-ichi Wada, Yuji Baba
    • Organizer
      The 23rd Hiroshima International Symposium on Synchrotron Radiation
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Brを含むDNA関連分子の内殻電子状態の研究2019

    • Author(s)
      平戸未彩紀, 横谷明徳, 馬場祐治, 藤井健太郎
    • Organizer
      2018年度量子ビームサイエンスフェスタ
    • Related Report
      2018 Research-status Report
  • [Presentation] XANES spectral changes of hydrated deoxyribose induced by K-ionization of oxygen2018

    • Author(s)
      Kentaro Fujii, Akinari Yokoya
    • Organizer
      International Conference on Synchrotron Radiation Instrumentation
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Proton transfers between a deoxyribose and hydrated waters after K-ionizations of constituent atoms2018

    • Author(s)
      Kentaro Fujii, Akinari Yokoya, M-A. Herve du Penhoat, M-F. Politis
    • Organizer
      15th International Workshop on Radiation Damage to DNA
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 酸素K殻イオン化によって起こるデオキシリボースの分解過程2018

    • Author(s)
      藤井健太郎, 横谷明徳, M-A. Herve du Penhoat, M-F. Politis
    • Organizer
      第12回分子化学討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] X線光電子分光法によるBrを含むDNA関連分子の内殻電子状態の研究2018

    • Author(s)
      平戸未彩紀, 横谷明徳, 馬場祐治, 藤井健太郎
    • Organizer
      日本放射線影響学会第61回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 電離放射線によって生じるDNA鎖切断 に対する水和水分子の役割2018

    • Author(s)
      藤井 健太郎, 横谷 明徳, M-A Herve du Penhoat, M-F Politis
    • Organizer
      第31回日本放射光学会年会・放射光科学合同シンポジウム
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 放射光円二色性分光によるメチル化ヒストンH3の構造解析2018

    • Author(s)
      泉 雄大, 松尾 光一, 藤井 健太郎, 横谷 明徳
    • Organizer
      第31回日本放射光学会年会・放射光科学合同シンポジウム
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] VUV-CDスペクトル測定による DNA修復タンパク質 XRCC4の二次構造解析2018

    • Author(s)
      西久保 開, 泉 雄大, 藤井 健太郎, 松尾 光一, 松本 義久, 横谷 明徳
    • Organizer
      第31回日本放射光学会年会・放射光科学合同シンポジウム
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Secondary structural analysis of XRCC4 protein using HiSOR-VUVCD2018

    • Author(s)
      Kai Nishikubo, Yudai Izumi, Kentaro Fujii, K.Matsuo, Y.Yamamoto, Akinari Yokoya
    • Organizer
      The 22nd Hiroshima International Symposium on Synchrotron Radiation
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Radiation Damage to DNA Induced by K-shell Electron Ionizations2018

    • Author(s)
      藤井 健太郎
    • Organizer
      HiSORセミナー
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 円二色性スペクトル法によるDNA2本鎖切断修復関連 タンパク質(XRCC4)の二次構造解析2017

    • Author(s)
      西久保 開, 泉 雄大, 藤井 健太郎, 松本 義久, 横谷 明徳
    • Organizer
      科学技術創成研究院 先導原子力研究所 研究交流・発表会
    • Related Report
      2017 Research-status Report
  • [Presentation] Structural Analysis of DNA Repair Protein (XRCC4) by Applying Circular Dichroism at Synchrotron VUV Beamline2017

    • Author(s)
      Kai Nishikubo, Yudai Izumi, Kentaro Fujii, Yoshihisa Matsumoto, Akinari Yokoya
    • Organizer
      第1回国際シンポジウム 「量子生命科学 -Quantum Life Science-」
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] シンクロトロン放射を用いたCDスペクトル測定によるDNA修復タンパク質XRCC4の構造解析2017

    • Author(s)
      西久保 開, 泉 雄大, 藤井 健太郎, 松本 義久, 横谷 明徳
    • Organizer
      平成29年度KUR専門研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] Ultrafast Proton Transfer between DNA-Sugar Molecule and Hydrated Water induced by K-Ionization of Oxygen2017

    • Author(s)
      藤井 健太郎, Marie-Francois Politis, Marie-Anne Herve du Penhoat, 横谷 明徳
    • Organizer
      第1回国際シンポジウム 「量子生命科学 -Quantum Life Science-」
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] シンクロトロン放射を用いたCD スペクトル測定によるDNA 修復タンパク質XRCC4 の溶液中での構造解析2017

    • Author(s)
      西久保 開, 泉 雄大, 藤井 健太郎, 松本 義久, 横谷 明徳
    • Organizer
      日本放射線影響学会
    • Related Report
      2017 Research-status Report
  • [Presentation] STRUCTURAL ANALYSIS OF DNA REPAIR PROTEIN (XRCC4) APPLYING CIRCULAR DICHROISM IN AN AQUEOUS SOLUTION2017

    • Author(s)
      西久保 開, 泉 雄大, 藤井 健太郎, 松本 義久, 横谷 明徳
    • Organizer
      MICROS 2017 - 17th International Symposium on Microdosimetry 参加
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] DNA構成元素の内殻励起によって誘発されるDNA損傷の定量実験2017

    • Author(s)
      鯉渕 誠也, 藤井 健太郎, 横谷 明徳
    • Organizer
      平成29年度KUR専門研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] Quantum Life Sciences in QST2017

    • Author(s)
      Akinari Yokoya, Tatsuhiko Imaoka, Takeshi Ohshima, Shinobu Onoda, Kenji Owada, Kentaro Fujii, Koichi Yamakawa, Motoyasu Adachi, Hironori Oba, Makiko Yamada, Hidetoshi Kono, Hiroshi Murakami, Noriyuki Ouchi, Yoshiteru Yonetani, Ryuji Igarashi, Yudai Izumi, Shigenori Tanaka, Mutshuko Hatano, Shigeki Takeuhi
    • Organizer
      第1回QST国際シンポジウム「量子生命科学 -Quantum Life Science-」
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Fragmentation of Doubly-Ionized Deoxyribose.2017

    • Author(s)
      Marie-Annne Herve du Penhoat, Alexandre souchaudl, Rodolphe Vuilleumier, Marie-Piene Gaigeot, Kentaro Fujii, Akinari Yokoya, Marie-Francoise politis
    • Organizer
      第1回QST国際シンポジウム「量子生命科学 -Quantum Life Science-」
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Synchrotron Radiation as a Quantum Tool for Studies of Radiation Damage to DNA and Resulting Cellular Responses2017

    • Author(s)
      横谷 明徳, 藤井 健太郎, 島田 紘行, 鵜飼 正敏, 神長 輝一, Kenji Owada, 町田 晃彦, 西村 由希子, 今岡 達彦
    • Organizer
      Conbio2017(生命科学系学会合同年次大会)
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2022-01-27  

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