Human exposure and disposition of naturally occuring brominated biphenols and anthropogenic persistent organohalogens

• Project/Area Number: 17K00574
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Daiichi University, College of Pharmaceutical Sciences
• Principal Investigator: Haraguchi Koichi 第一薬科大学, 薬学部, 教授 (00258500)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 体内動態 / 臭素化合物 / 海藻 / 天然化合物 / 曝露量 / GC/MS / 母乳 / ヒト / 残留

Outline of Final Research Achievements

The origin of naturally produced and persistent brominated biphenyls (OH-BDEs) was found to be marine alga (Sargassum sp.) from east Asia. These OH-BDEs were accumulated as methoxylated products in higher trophic organisms such as cetaceans, followed by fish. Human exposure to MeO-BDEs via diet may result in hepatic metabolism by CYP2B6 to OH-BDEs, of which 6-OH-BDE47 may be retained in blood of human.

Academic Significance and Societal Importance of the Research Achievements

ヒトや海洋哺乳動物には残留性有機汚染物質(POPs)と同レベルで天然由来の臭素化ビフェノール類(OH-BDE)が広範囲で検出されている。本研究では、海藻由来のOH-BDEのメチル化体が、食物連鎖により魚介類やヒト食事に検出されること、またヒトに取り込まれたMeO-PBDEは容易に脱メチル化されやすいことを明らかにした。OH-BDEは、抗菌・抗酸化能や内分泌かく乱性を有すると考えられ、そのヒト影響研究の基礎資料となる。

Research Products

• [Journal Article] Estimation of dietary intake and sources of organohalogenated contaminants among infants: 24-h duplicate diet survey in Fukuoka, Japan (2021)
  • Author(s): Fujii Y, Poma G, Malarvannan G, Soeda F, Toda A, Haraguchi K, Covaci A.
  • Journal Title: Environmental Research Volume: 195 Pages: 110745-110745
  • DOI: 10.1016/j.envres.2021.110745
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effects of perfluoroalkyl carboxylic acids on the uptake of sulfobromophthalein via organic anion transporting polypeptides in human intestinal Caco-2 cells (2020)
  • Author(s): Kimura Osamu、Fujii Yukiko、Haraguchi Koichi、Kato Yoshihisa、Ohta Chiho、Koga Nobuyuki、Endo Tetsuya
  • Journal Title: Biochemistry and Biophysics Reports Volume: 24 Pages: 100807-100807
  • DOI: 10.1016/j.bbrep.2020.100807
  • Peer Reviewed / Open Access
• [Journal Article] Perfluorinated carboxylic acids in edible clams: A possible exposure source of perfluorooctanoic acid for Japanese population (2020)
  • Author(s): Fujii Yukiko、Harada Kouji H.、Nakamura Tsubasa、Kato Yoshihisa、Ohta Chiho、Koga Nobuyuki、Kimura Osamu、Endo Tetsuya、Koizumi Akio、Haraguchi Koichi
  • Journal Title: Environmental Pollution Volume: 263 Pages: 114369-114369
  • DOI: 10.1016/j.envpol.2020.114369
  • Peer Reviewed
• [Journal Article] Lactational transfer of long-chain perfluorinated carboxylic acids in mice: a method to directly collect milk and evaluate chemical transferability (2020)
  • Author(s): Fujii Y, Harada K, Kobayashi H, Haraguchi K, Koizumi A.
  • Journal Title: Toxics Volume: 8 Issue: 2 Pages: 23-23
  • DOI: 10.3390/toxics8020023
  • Peer Reviewed / Open Access
• [Journal Article] 2,2',3,4',5,6,6'-七塩素化ビフェニル(CB188)のラットおよびモルモット肝ミクロゾームによる代謝 (2019)
  • Author(s): 太田千穂、山本健太、加藤善久、藤井由希子、原口浩一、木村治、遠藤哲也、古賀信幸
  • Journal Title: 福岡医学雑誌 Volume: 110 Pages: 83-90
  • Peer Reviewed
• [Journal Article] Assessment of diabetics by the quantification of essential elements and stable isotope ratios of carbon and nitrogen in scalp hair (2019)
  • Author(s): Hotta Yohei、Fujino Ryoji、Kimura Osamu、Fujii Yukiko、Haraguchi Koichi、Endo Tetsuya
  • Journal Title: Obesity Medicine Volume: 印刷中 Pages: 100106-100106
  • DOI: 10.1016/j.obmed.2019.100106
  • Peer Reviewed
• [Journal Article] Kanechlor 500-mediated changes in serum and hepatic thyroxine levels primarily occur in a transthyretin-unrelated manner (2019)
  • Author(s): Kato Y, Tamaki S, Haraguchi K, Ikushiro S, Fujii Y, Ohta C, Atobe K, Kimura O, Endo T, Koga N, Yamada S, Degawa M
  • Journal Title: Journal of Applied Toxicology Volume: 39 Issue: 12 Pages: 1701-1709
  • DOI: 10.1002/jat.3895
  • Peer Reviewed
• [Journal Article] Levels and profiles of long-chain perfluoroalkyl carboxylic acids in Pacific cod from 14 sites in the North Pacific Ocean (2019)
  • Author(s): Fujii Yukiko、Tuda Hayato、Kato Yoshihisa、Kimura Osamu、Endo Tetsuya、Harada Kouji H.、Koizumi Akio、Haraguchi Koichi
  • Journal Title: Environmental Pollution Volume: 247 Pages: 312-318
  • DOI: 10.1016/j.envpol.2019.01.006
  • Peer Reviewed
• [Journal Article] Different profiles of naturally produced and anthropogenic organohalogens in the livers of cetaceans from the Sea of Japan and the North Pacific Ocean (2018)
  • Author(s): Fujii Yukiko、Kato Yoshihisa、Kozai Mai、Matsuishi Takashi、Harada Kouji H.、Koizumi Akio、Kimura Osamu、Endo Tetsuya、Haraguchi Koichi
  • Journal Title: Marine Pollution Bulletin Volume: 136 Pages: 230-242
  • DOI: 10.1016/j.marpolbul.2018.08.051
  • Peer Reviewed
• [Journal Article] Comparison of radiocesium and stable isotope ratios of carbon and nitrogen among three stocks of Pacific cod (Gadus macrocephalus) around Hokkaido, Japan (2018)
  • Author(s): Nakamura Tsugiya、Tanaka Toshiyasu、Kimura Osamu、Fujii Yukiko、Haraguchi Koichi、Endo Tetsuya
  • Journal Title: Marine Pollution Bulletin Volume: 127 Pages: 39-44
  • DOI: 10.1016/j.marpolbul.2017.11.029
  • Peer Reviewed
• [Journal Article] Tissue-specific bioaccumulation of long-chain perfluorinated carboxylic acids and halogenated methylbipyrroles in Dall's porpoises (Phocoenoides dalli) and harbor porpoises (Phocoena phocoena) stranded in northern Japan. (2018)
  • Author(s): Fujii Y, Kato, Sakamoto KMatsuishi T, Kouji KH, Koizumi A, Kimura O, ENdo T, Haraguchi K.
  • Journal Title: Science of the Total Environment Volume: 616-617 Pages: 554-563
  • Peer Reviewed
• [Journal Article] Contamination trends and factors affecting the transfer of hexabromocyclododecane diastereomers, tetrabromobisphenol A, and 2,4,6-tribromophenol to breast milk in Japan. (2018)
  • Author(s): Fujii Y, Kato Y, Masuda N, Harada KH, Koizumi A, Haraguchi K.
  • Journal Title: Environmental Pollution Volume: 237 Pages: 936-943
  • DOI: 10.1016/j.envpol.2018.03.015
  • Peer Reviewed
• [Presentation] 2,2’,4,4’,6,6’-六塩素化ビフェニル(PCB155)のヒト肝ミクロゾームおよびチトクロムP450分子種による代謝 (2021)
  • Author(s): 太田 千穂、加藤 善久、藤井 由希子、原口 浩一、木村 治、遠藤 哲也、古賀 信幸
  • Organizer: 日本薬学会第141年会
• [Presentation] Occurrence of Anthropogenic and Naturally Produced Organohalogens in Baby Food Duplicates for Japanese Infants (2019)
  • Author(s): Fujii Y, Poma G, Malarvannan G, Haraguchi K, Covaci A
  • Organizer: Dioxin 2019
  • Int'l Joint Research
• [Presentation] Seafood in Lower Trophic Level is a Possible Source of Human Dietary Exposure：Perfluorinated Carboxylic Acids in Manila Clam (ruditapes philippinarum) in Japan (2019)
  • Author(s): Fujii Y, Nakamura T, Kato Y, Ohta C, Koga N, Kimura O, Endo T, Harada KH, Koizumi A, Haraguchi K
  • Organizer: Dioxin 2019
  • Int'l Joint Research
• [Presentation] Edible fish is a source of human dietary exposure: perfluorinated alkyl acids in Pacific cods from North Pacific Ocean. (2018)
  • Author(s): Fujii Y, Haraguchi K, Kato Y, Ohta C, Koga N, Kimura O, Endo T, Harada KH, Koizumi A
  • Organizer: Dioxin 2018: 38th International Symposium & 10th international PCB workshop.
• [Presentation] In vitro metabolism of 2,2',3,4',5,6,6'-heptachlorobiphenyl (CB188) by rat liver microsomes. (2018)
  • Author(s): Ohta C, Yamamoto K, Fujii Y, Haraguchi K, Kimura O, Endo T, Kato Y, Koga N.
  • Organizer: Dioxin 2018: 38th International Symposium & 10th international PCB workshop.
• [Presentation] Involvement of transthyretin to Kanechlor-500-mediated changes in serum and hepatic thyroxine levels in mice. (2018)
  • Author(s): Kato Y, Tamaki S, Haraguchi K, Fujii Y, Kimura O, Ohta C, Endo T, Koga N, Degawa M.
  • Organizer: Dioxin 2018: 38th International Symposium & 10th international PCB workshop.
• [Presentation] Human exposure to brominated phenoxy phenols: seaweeds as source of hydroxylated and methoxylated PBDEs in Asia-Pacific (2018)
  • Author(s): Haraguchi K, Fujii Y, Ohta C, Koga N, Kimura O, Endo T, Kato Y.
  • Organizer: Dioxin 2018: 38th International Symposium & 10th international PCB workshop.
• [Presentation] Brominated catechols and guaiacols as possible sources of dihydroxylated PBDEs and hydroxylated dioxins in bivalves from Asia-Pacific (2017)
  • Author(s): Haraguchi K, Fujii Y, Kimura O, Endo T, Kato Y, Ohta C, Koga N
  • Organizer: Dioxin 2017、Vancouver Canada
• [Presentation] 環境化学物質による女性ホルモン機能の修飾メカニズム (2017)
  • Author(s): 岡崎裕之、竹田修三、藤井由希子、原口浩一、渡辺和人、荒牧弘範.
  • Organizer: 第44回日本毒性学会学術大会（横浜）
• [Presentation] 海藻（Sargassum fusiforme）に含有される臭素化ビフェノール（6-OH-BDE47）の抗菌作用 (2017)
  • Author(s): 藤井由希子、小川和加野、松原 大、原口浩一
  • Organizer: 第51回ビブリオシンポジウム（石垣）
• [Book] Marine Environments: Diversity, Threats and Conservation (2020)
  • Author(s): Endo T, Matsuishi T, Fujii Y, Haraguchi K
  • Total Pages: 58
  • Publisher: Nova (New York)
  • ISBN: 9781536188745