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Development of prediction method for mass death of fish by using eRNA and stress hormone

Research Project

Project/Area Number 17K00581
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental impact assessment
Research InstitutionShimane University

Principal Investigator

Takahara Teruhiko  島根大学, 学術研究院農生命科学系, 准教授 (10536048)

Co-Investigator(Kenkyū-buntansha) 土居 秀幸  兵庫県立大学, シミュレーション学研究科, 准教授 (80608505)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords環境DNA / 環境RNA / ストレスホルモン / 水生動物 / コルチゾール / 環境mRNA / 環境DNAメタバーコーディング / 宍道湖 / DNA断片長 / 魚介類 / 大量死 / 環境技術 / 環境分析 / 環境変動 / 生態学
Outline of Final Research Achievements

In this study, by using environmental DNA (eDNA), environmental RNA (eRNA), and stress hormones (cortisol), we tried to develop the method for assessing the risk of biological mass death and the predictive monitoring in aquatic ecosystems. Lake Shinji as our main field is a shallow oligohaline lake on the coast of the Sea of Japan. The samples for eDNA, eRNA, and stress hormone were accumulated at 14 sites/month throughout 3 study years. In addition, the optimal storage method for water samples for eDNA was clarified. And, a method for collecting, concentrating, and purifying eRNA was established. We also established the method for assessing the amount of cortisol (stress hormone) released from the target organism under stress experimental conditions. Based on these results, we believe that we have established the basic technology necessary to perform the risk assessment of aquatic mass mortality.

Academic Significance and Societal Importance of the Research Achievements

本研究では、これまでほとんど明らかにされていなかった湖沼などにおける魚介類の大量死イベントに関して、予測とモニタリングを可能にする技術開発に取り組んでおり、学術的意義は大きいと考えている。具体的には、水生動物を用いた詳細な飼育実験と継続的な野外調査を通して、大量死の予兆を把握するために環境RNAやストレスホルモンの測定を可能にした点があげられる。本研究で開発された技術は、様々な水生動物にも即座に応用可能であり、今後の発展として、水域生態系における大量死予測に関する汎用的な手法を提案し、重要水産資源等の持続可能な管理の実現が期待できると考えている。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (23 results)

All 2020 2019 2018 2017

All Journal Article (17 results) (of which Peer Reviewed: 13 results,  Open Access: 9 results) Presentation (6 results)

  • [Journal Article] Comparison of the detection of 3 endangered frog species by eDNA and acoustic surveys across 3 seasons2020

    • Author(s)
      Takahara Teruhiko、Iwai Noriko、Yasumiba Kiyomi、Igawa Takeshi
    • Journal Title

      Freshwater Science

      Volume: 39 Issue: 1 Pages: 18-27

    • DOI

      10.1086/707365

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Using environmental DNA to estimate the seasonal distribution and habitat preferences of a Japanese basket clam in Lake Shinji, Japan2019

    • Author(s)
      Takahara Teruhiko、Ikebuchi Takashi、Doi Hideyuki、Minamoto Toshifumi
    • Journal Title

      Estuarine, Coastal and Shelf Science

      Volume: 221 Pages: 15-20

    • DOI

      10.1016/j.ecss.2019.02.022

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] The detection of aquatic macroorganisms using environmental DNA analysis?A review of methods for collection, extraction, and detection2019

    • Author(s)
      Tsuji Satsuki、Takahara Teruhiko、Doi Hideyuki、Shibata Naoki、Yamanaka Hiroki
    • Journal Title

      Environmental DNA

      Volume: 1 Issue: 2 Pages: 99-108

    • DOI

      10.1002/edn3.21

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An application of PCR-RFLP species identification assay for environmental DNA detection2019

    • Author(s)
      Igawa Takeshi、Takahara Teruhiko、Lau Quintin、Komaki Shohei
    • Journal Title

      PeerJ

      Volume: 7 Pages: e7597-e7597

    • DOI

      10.7717/peerj.7597

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 環境DNAを用いた水域生態系の解明に向けた取り組み2019

    • Author(s)
      高原輝彦
    • Journal Title

      用水と排水

      Volume: 61 Pages: 64-70

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
  • [Journal Article] Efficacy of environmental DNA to detect and quantify stream tadpoles of Odorrana splendida2019

    • Author(s)
      Iwai Noriko、Yasumiba Kiyomi、Takahara Teruhiko
    • Journal Title

      Royal Society Open Science

      Volume: 6 Issue: 1 Pages: 181798-181798

    • DOI

      10.1098/rsos.181798

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of detection probabilities at the water-filtering and initial PCR steps in environmental DNA metabarcoding using a multispecies site occupancy model2019

    • Author(s)
      Doi Hideyuki、Fukaya Keiichi、Oka Shin-ichiro、Sato Keiichi、Kondoh Michio、Miya Masaki
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 3581-3581

    • DOI

      10.1038/s41598-019-40233-1

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Environmental DNA metabarcoding for fish community analysis in backwater lakes: A comparison of capture methods2019

    • Author(s)
      Fujii Kazuya、Doi Hideyuki、Matsuoka Shunsuke、Nagano Mariko、Sato Hirotoshi、Yamanaka Hiroki
    • Journal Title

      PLOS ONE

      Volume: 14 Issue: 1 Pages: e0210357-e0210357

    • DOI

      10.1371/journal.pone.0210357

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] An analytical framework for estimating aquatic species density from environmental DNA2018

    • Author(s)
      Chambert Thierry、Pilliod David S.、Goldberg Caren S.、Doi Hideyuki、Takahara Teruhiko
    • Journal Title

      Ecology and Evolution

      Volume: 8 Issue: 6 Pages: 3468-3477

    • DOI

      10.1002/ece3.3764

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] 水生動物の生物量、季節分布と移動における環境DNAを用いた推定2017

    • Author(s)
      高原輝彦
    • Journal Title

      環境技術

      Volume: 46 Pages: 636-641

    • Related Report
      2017 Research-status Report
  • [Journal Article] 環境DNAモニタリング手法の課題と展望2017

    • Author(s)
      源利文、内井喜美子、高原輝彦、土居秀幸
    • Journal Title

      環境技術

      Volume: 46 Pages: 648-652

    • Related Report
      2017 Research-status Report
  • [Journal Article] 流水中におけるカワムツの生物量と環境DNA量の関係性-水路実験と野外への適用-2017

    • Author(s)
      乾隆帝、赤松良久、高原輝彦、後藤益滋、一松晃弘
    • Journal Title

      河川技術論文集

      Volume: 23 Pages: 651-656

    • Related Report
      2017 Research-status Report
  • [Journal Article] Isopropanol precipitation method for collecting fish environmental DNA2017

    • Author(s)
      Doi Hideyuki、Uchii Kimiko、Matsuhashi Saeko、Takahara Teruhiko、Yamanaka Hiroki、Minamoto Toshifumi
    • Journal Title

      Limnology and Oceanography: Methods

      Volume: 15 Issue: 2 Pages: 212-218

    • DOI

      10.1002/lom3.10161

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Usefulness and limitations of sample pooling for environmental DNA metabarcoding of freshwater fish communities2017

    • Author(s)
      Sato Hirotoshi、Sogo Yuki、Doi Hideyuki、Yamanaka Hiroki
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 14860-14860

    • DOI

      10.1038/s41598-017-14978-6

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Distinct seasonal migration patterns of Japanese native and non-native genotypes of common carp estimated by environmental DNA2017

    • Author(s)
      Uchii Kimiko、Doi Hideyuki、Yamanaka Hiroki、Minamoto Toshifumi
    • Journal Title

      Ecology and Evolution

      Volume: 7 Issue: 20 Pages: 8515-8522

    • DOI

      10.1002/ece3.3346

    • NAID

      120006358583

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Detection of an endangered aquatic heteropteran using environmental DNA in a wetland ecosystem2017

    • Author(s)
      Doi Hideyuki、Katano Izumi、Sakata Yusuke、Souma Rio、Kosuge Toshihiro、Nagano Mariko、Ikeda Kousuke、Yano Koki、Tojo Koji
    • Journal Title

      Royal Society Open Science

      Volume: 4 Issue: 7 Pages: 170568-170568

    • DOI

      10.1098/rsos.170568

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Environmental DNA method for estimating salamander distribution in headwater streams, and a comparison of water sampling methods2017

    • Author(s)
      Katano Izumi、Harada Ken、Doi Hideyuki、Souma Rio、Minamoto Toshifumi
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 5 Pages: e0176541-e0176541

    • DOI

      10.1371/journal.pone.0176541

    • NAID

      120006355667

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 環境DNAメタバーコーディングを用いた宍道湖-中海の魚類・鳥類群集の季節変遷解析2019

    • Author(s)
      高原 輝彦、山中 裕樹、折戸 みゆき、中道 友規、立石 新・土居 秀幸、後藤 亮、佐土 哲也、宮 正樹
    • Organizer
      第2回環境DNA学会神戸大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 環境DNAメタバーコーディング解析を用いた微生物群集の空間構造解析:群集生態学の高次プロセスを微生物群集から考える2019

    • Author(s)
      中島 広樹、土居 秀幸、高原 輝彦、松岡 俊将、永野 真理子
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 環境DNA分析を利用した宍道湖-中海に生息する魚介類の生態解明への試み2018

    • Author(s)
      高原輝彦、山岸聖、田口淳也、池淵貴志、立石新、尾形茂紀、稲岡悠樹、服部真也、黒瀬裕斗、藤井正人、土居秀幸、山中裕樹、源利文
    • Organizer
      第1回環境DNA学会東京大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 環境DNAを用いたため池における希少昆虫タガメと侵略的外来種の関係調査2018

    • Author(s)
      尾形茂紀、西脇淳浩、山添寛治、土居秀幸、源利文、須貝杏子、高原輝彦
    • Organizer
      第1回環境DNA学会東京大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 環境DNAを用いた宍道湖ヤマトシジミ資源量推定への試み2018

    • Author(s)
      高原輝彦、田口淳也、池淵貴志、内田浩、石田健次、山岸聖、尾形茂紀、土居秀幸、源利文
    • Organizer
      第65回日本生態学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 環境DNAを用いたため池におけるタガメの分布状況推定2018

    • Author(s)
      尾形茂紀、西脇淳浩、山添寛治、須貝杏子、高原輝彦
    • Organizer
      第65回日本生態学会大会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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