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Mechanism of cesium accumulation in Paramecium bursaria

Research Project

Project/Area Number 17K00613
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Modeling and technologies for environmental conservation and remediation
Research InstitutionKobe University

Principal Investigator

Suzaki Toshinobu  神戸大学, 理学研究科, 准教授 (00187692)

Co-Investigator(Kenkyū-buntansha) 吉村 知里  神戸大学, 環境保全推進センター, 助教 (60362761)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords放射性セシウム / 除染 / 原生生物 / 油滴 / 細胞内共生 / クロレラ / ミドリゾウリムシ
Outline of Final Research Achievements

The protozoan Paramecium bursaria that contains intracellular symbiotic Chlorella was found to possess the ability to dissociated soil-bound cesium from contaminated soil and accumulate at high concentrations in intracellular oil droplet granules. This method was considered to be effective in treating contaminated soil with radioactive cesium in Fukushima Prefecture. In this study, we conducted the following. 1) A method was developed to selectively collect only P. bursaria after mixing the soil suspension with paramecia. 2) Oil droplets were isolated from P. bursaria and the protein composition contained therein was analyzed by mass spectrometry. As a result, proteins that may be involved in cesium transport were identified.

Academic Significance and Societal Importance of the Research Achievements

セシウムの除去法に関する技術は既に多数報告されているが、それらのほとんどは可溶性のセシウムを対象にしたものである。従って、汚染土壌の現場処理法としてミドリゾウリムシを用いた本法は有用性が高く、独創的である。他の生物と比較してミドリゾウリムシがバイオレメディエーションのための生物として優れている理由は、この生物が、細胞口から細胞内に運び込んだ土壌粒子を細胞内でpH 3程度にまで強く酸性化する能力を有している点にある。ミドリゾウリムシは、セシウム以外にも各種重金属を取り込むという報告もあるので、この技術は、セシウムに限らず様々な有害金属(あるいは有用な金属)の除去法もに発展する可能性がある。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (37 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (12 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 12 results,  Open Access: 11 results) Presentation (23 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Ansar/VDP Training Center(バングラデシュ)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Pediludiella daitoensis gen. et sp. nov. (Scenedesmaceae, Chlorophyceae), a large coccoid green alga isolated from a Loxodes ciliate2020

    • Author(s)
      Ryo Hoshina, Masashi M Hayakawa, Mayumi Kobayashi, Rina Higuchi, Toshinobu Suzaki
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 628-628

    • DOI

      10.1038/s41598-020-57423-x

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Taming chlorophylls allowed eukaryotes to prosper on the oxygenated Earth.2019

    • Author(s)
      Kashiyama Y, Yokoyama A, Shiratori T, Bachy C, Gutierrez-Rodriguez A, Not F, Hess S, Wang M, Chen M, Gong Y, Seto K, Kagami M, Hamamoto Y, Honda D, Umetani T, Shihongi A, Kayama M, Matsuda T, Taira J, Yabuki A, Tsuchiya M, Hirakawa Y, Kawaguchi A, Nomura M, Nakamura A, Namba N, Matsumoto M, Tanaka T, Yoshino T et al.
    • Journal Title

      The ISME journal

      Volume: - Issue: 8 Pages: 1-12

    • DOI

      10.1038/s41396-019-0377-0

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea)2018

    • Author(s)
      Rina Higuchi, Chihong Song, Ryo Hoshina and ToshinobuSuzaki
    • Journal Title

      Eur. J. Protistol.

      Volume: 66 Pages: 149-155

    • DOI

      10.1016/j.ejop.2018.09.003

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Putative Neural Network Within an Olfactory Sensory Unit for Nestmate and Non-nestmate Discrimination in the Japanese Carpenter Ant: The Ultra-structures and Mathematical Simulation2018

    • Author(s)
      Yusuke Takeichi, Tatsuya Uebi, Naoyuki Miyazaki, Kazuyoshi Murata, Kouji Yasuyama, Kanako Inoue, Toshinobu Suzaki, Hideo Kubo, Naoko Kajimura, Jo Takano, Toshiaki Omori, Ryoichi Yoshimura, Yasuhisa Endo, Masaru K. Hojo, Eichi Takaya, Satoshi Kurihara, Kenta Tatsuta, Koichi Ozaki, Mamiko Ozaki
    • Journal Title

      Frontiers in Cellular Neuroscience

      Volume: 12 Pages: 1-1

    • DOI

      10.3389/fncel.2018.00310

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bioconjugation strategy for cell surface labelling with gold nanostructures designed for highly localized pH measurement.2018

    • Author(s)
      Leonardo Puppulin, Shigekuni Hosogi, Hongxin Sun, Kazuhiko Matsuo, Toshio Inui, Yasuaki Kumamoto, Toshinobu Suzaki, Hideo Tanaka and Yoshinori Marunaka
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 5278-5278

    • DOI

      10.1038/s41467-018-07726-5

    • NAID

      120006549200

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Intracellular symbiosis of algae with possible involvement of mitochondrial dynamics2017

    • Author(s)
      Chihong Song, Kazuyoshi Murata and Toshinobu Suzaki
    • Journal Title

      Scientific Repots

      Volume: 7 Issue: 1 Pages: 1221-1221

    • DOI

      10.1038/s41598-017-01331-0

    • NAID

      120006348188

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Diacylglycerol kinase alpha is involved in the vitamin E-induced amelioration of diabetic nephropathy in mice2017

    • Author(s)
      Daiki Hayashi1 Keiko Yagi, Chihong Song, Shuji Ueda, Minoru Yamanoue, Matthew Topham, Toshinobu Suzaki, Naoaki Saito, Noriaki Emoto and Yasuhito Shirai
    • Journal Title

      Scientific Repots

      Volume: 7 Issue: 1 Pages: 2597-2597

    • DOI

      10.1038/s41598-017-02354-3

    • NAID

      120006348186

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Major vault protein of the protozoan Raphidiophrys contractilis has a binding property to β-1,3-glucan and is involved in food capturing2017

    • Author(s)
      Bhadra, M., Kobayashi, M., Higuchi, R., Chen, L. and Suzaki, T.
    • Journal Title

      Int. J. New Tech. Res.

      Volume: 3 Pages: 1-7

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Brandtia ciliaticola gen. et sp. nov. (Chlorellaceae, Trebouxiophyceae) a common symbiotic green coccoid among various ciliate species2017

    • Author(s)
      Ryo Hoshina, Mayumi Kobayashi, Toshinobu Suzaki, Yasushi Kusuoka
    • Journal Title

      Phycol. Res.

      Volume: 66 Issue: 1 Pages: 76-81

    • DOI

      10.1111/pre.12194

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a compact, highly-sensitive and low-cost biological monitoring method using protozoa for detecting toxicants in aquatic environment2017

    • Author(s)
      Yoshimura, C., Kobayashi, M., Khan, S.M.M.K., Islam, M.D.S., Matsubara, S., Chen, L., Higuchi, R. and Suzaki, T.
    • Journal Title

      Int. J. Environ. Agr. Res.

      Volume: 3 Issue: 8 Pages: 41-44

    • DOI

      10.25125/agriculture-journal-ijoear-jul-2017-7

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Inositol hexakis phosphate is the seasonal phosphorus reservoir in the deciduous woody plant Populus alba L.2017

    • Author(s)
      Kurita, Y., Baba, K., Ohnishi, M., Matsubara, R., Kosuge, K., Anegawa, A., Shichijo, C., Ishizaki, K., Kaneko, Y., Hayashi, M., Suzaki, T., Fukaki, H., Mimura, T.
    • Journal Title

      Plant Cell Physiol.

      Volume: 58 Issue: 9 Pages: 1477-1485

    • DOI

      10.1093/pcp/pcx106

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Glomerular Hypertrophy and Hyperfiltration in Obesity-Related Diabetic (ob/ob) Mouse2017

    • Author(s)
      Tomonori Tokunaga, Yuri Fujiwara, Mai Matsushita, Sanae Horie, Toshinobu Suzaki, Seiichi Osaki, Tetsuaki Matsuhisa and Etsuko Suzaki
    • Journal Title

      Analytical and Quantitative Cytopathology and Histopathology

      Volume: 39 Pages: 223-230

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] ミドリゾウリムシに共生できないクロレラ変異株の分子的・構造的特徴2019

    • Author(s)
      石澤雪香,樋口里樹,ソンチホン,洲崎 敏伸
    • Organizer
      第3回日本共生生物学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] グリーンヒドラはクロレラを共生させることで周囲の藻類を除去する能力を獲得する2019

    • Author(s)
      竹中宏規,北川円,洲崎 敏伸
    • Organizer
      第3回日本共生生物学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 渦鞭毛藻Oxyrrhis Marinaの核に関する構造的研究2019

    • Author(s)
      ソンチホン,福田康弘,村田和義,洲崎敏伸
    • Organizer
      第52回日本原生生物学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ハリタイヨウチュウRaphidiophrys contractilisのケイ酸質被殻の機能に関する研究2019

    • Author(s)
      大谷咲会,長尾賢杜,有川幹彦,ソンチホン,洲崎 敏伸
    • Organizer
      第52回日本原生生物学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Ultrastructure of the kinetocyst in the heliozoon Raphidiophrys contractilis2019

    • Author(s)
      WAN Yumeng, SUZAKI Toshinobu, KOBAYASHI Mayumi
    • Organizer
      第52回日本原生生物学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Possible involvement of mitochondria in Chlorella-Paramecium bursaria endosymbiosis2019

    • Author(s)
      YANG Xin, SUZAKI Toshinobu
    • Organizer
      第52回日本原生生物学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Structure and function of the scale of Raphidiophrys contractilis.2018

    • Author(s)
      Sakie Otani, Kento Nagao, Mikihiko Arikawa and Toshinobu Suzaki.
    • Organizer
      Joint Meeting of the Japan Society of Protistology and the Korean Society of Protozoologists
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Major vault protein, a protein for prey recognition in Raphidiophrys contractilis2018

    • Author(s)
      Munehiro Karasawa, Mayumi Kobayashi, Toshinobu Suzaki.
    • Organizer
      Joint Meeting of the Japan Society of Protistology and the Korean Society of Protozoologists
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 太陽虫Raphidiophrys contractilisのケイ酸質被殻の機能2018

    • Author(s)
      洲崎 敏伸,大谷 咲会,長尾 賢杜,有川 幹彦
    • Organizer
      第51回日本原生生物学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 盗葉緑体の一生:Rapaza viridisが示唆するオルガネラ獲得進化の側面2018

    • Author(s)
      丸山萌,小林滉宜,粟井光一郎,谷藤吾朗,洲崎敏伸,柏山祐一郎
    • Organizer
      第42回日本藻類学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 有中心粒太陽虫ハリタイヨウチュウの細胞外被殻の構造と形成機構2017

    • Author(s)
      洲崎敏伸,長尾賢杜
    • Organizer
      第88回日本動物学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ハリタイヨウチュウにおける細胞小器官キネトシストの微細立体構造の決定と構成分子の同定に向けて2017

    • Author(s)
      小林真弓,Bhadra, M., 洲崎敏伸
    • Organizer
      第88回日本動物学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ミドリゾウリムシと共生クロレラを用いた細胞内共生構築に関わる分子機構の探索2017

    • Author(s)
      樋口里樹,洲崎敏伸
    • Organizer
      第88回日本動物学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 盗葉緑体の使用期限:Rapaza viridisの光合成活性2017

    • Author(s)
      丸山 萌,小林 滉宜,粟井光一郎,洲崎 敏伸,柏山 祐一郎
    • Organizer
      第1回日本共生生物学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ハリタイヨウチュウの捕食に関わるMVP2017

    • Author(s)
      唐澤宗宏,Mousumi BHADRA,小林真弓,洲崎敏伸
    • Organizer
      第1回日本共生生物学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Raphidiophrys contractilisの持つケイ酸質被殻の形成機構2017

    • Author(s)
      陳 林,千原あかね,小林真弓,長尾賢杜,洲崎敏伸
    • Organizer
      第1回日本共生生物学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] A new, compact, highly sensitive and low cost biological monitoring device using protozoa to detect toxic substances in water2017

    • Author(s)
      Islam, MD Shafiqul., 吉村知里,小林真弓,陳 林,樋口里樹,洲崎敏伸
    • Organizer
      第1回日本共生生物学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Chlorella variabilisの細胞表層タンパク質の細胞内共生に伴う変化2017

    • Author(s)
      樋口里樹,洲崎敏伸
    • Organizer
      第1回日本共生生物学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 原生生物Raphidiophrys contractilisの細胞小器官キネトシストの微細構造と分子構成2017

    • Author(s)
      小林真弓., ソンチホン, 村田和義, 洲崎敏伸
    • Organizer
      生理研ワークショップ「クライオ電子顕微鏡によるタンパク質の高分解能単粒子構造解析」
    • Related Report
      2017 Research-status Report
  • [Presentation] Structural and molecular basis for the interaction between endosymbiotic green algae and host protozoans2017

    • Author(s)
      Toshinobu Suzaki and Chihong Song
    • Organizer
      5th International Symposium on Plant Signaling and Behavior (PSB2017)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] An obligate kleptoplastic phototrophy of a euglenoid Rapaza viridis2017

    • Author(s)
      Moe Maruyama, Goro Tanifuji, Yuki Yazaki, Koichiro Awai, Toshinobu Suzaki and Yuichiro Kashiyama
    • Organizer
      15th International Congress of Protistology (ICOP 2017)ligate kleptoplastic phototrophy of a euglenoid Rapaza viridis
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Study on Cyclotrichium-like ciliate with sequestered diatom chloroplasts2017

    • Author(s)
      Hoshina, R., Kobayashi, M., Suzaki, T. and Kusuoka, Y.
    • Organizer
      15th International Congress of Protistology (ICOP 2017)ligate kleptoplastic phototrophy of a euglenoid Rapaza viridis
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Chlorb’ (Chlorellaceae, Trebouxiophyceae) a common symbiootc green coccoid of various ciliate species2017

    • Author(s)
      Hoshina, R., Kobayashi, M., Suzaki, T. and Kusuoka, Y.
    • Organizer
      The Asian Pacific Phycological Forum
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] 水棲生物の飼育装置2018

    • Inventor(s)
      洲崎敏伸、小林真弓、吉田恵史郎
    • Industrial Property Rights Holder
      洲崎敏伸、小林真弓、吉田恵史郎
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-049441
    • Filing Date
      2018
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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