Genetic engineering study on increasing the production rate of algal biofuel using a novel culture system
| Project/Area Number |
17K00676
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Design and evaluation of sustainable and environmental conscious system
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| Research Institution | Hiroshima National College of Maritime Technology |
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Principal Investigator |
Ohnuma Mio 広島商船高等専門学校, その他部局等, 准教授 (70594076)
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| Co-Investigator(Kenkyū-buntansha) |
三角 修己 山口大学, 大学院創成科学研究科, 准教授 (90583625)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 藻類バイオマス燃料 / 極限環境 / 単離 / オルガネラ / 油滴 / 細胞増殖 / 脂質生産 / ゲノム / 藻類バイオ燃料 / バイオマス利活用 / 藻類 / バイオ燃料 |
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| Outline of Final Research Achievements |
Based on comprehensive gene expression analysis under lipid accumulation condition that does not inhibit cell proliferation using primitive eukaryotic red algae, Cyanidioschyzon merolae, RT-PCR analysis was demonstrated for four genes that were considered to be particularly important for lipid production and accumulation. RT-PCR analysis showed strong upregulation of these four genes. It was suggested that the gene is useful for increasing lipid production, and the overexpressing strain can be used for lipid production.
In parallel with the above study, we isolated a novel lipid-producing alga suitable for a low-cost mass-culture system from a high-pH hot spring. The alga belongs to the green alga Chlorococcum and produces lipids containing mainly nine kinds of fatty acids. Among the obtained fatty acids, the fatty acid with a particularly high content was octadecenoic acid as other lipid-producing algae.
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| Academic Significance and Societal Importance of the Research Achievements |
高率的な藻類生産においては、脂質含有量が高く、増殖性の高い藻類が必須条件となる。ところが、一般の藻類では、細胞増殖と脂質の蓄積はトレードオフの関係があり、両立させることが困難である。シゾンを用いた遺伝子工学的研究により、細胞増殖をしながら脂質を蓄積する機構の基礎的知見が得られた。この知見は、広く藻類に適用できると考えられる。シゾンは培養温度40℃前後を好むため、当該遺伝子のシゾンの変異体は、脂質高生産株として、温泉地や工業施設などの近隣地域での藻類バイオ燃料生産に有用と考えられる。
並行して探索した新規脂質高生産藻類は、培養温度22℃前後を好むため、海洋などでの利用も視野に入れることができる。
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Glycosyltransferase MDR1 assembles a dividing ring for mitochondrial proliferation comprising polyglucan nanofilaments2017
Author(s)
Yoshida Yamato, Kuroiwa Haruko, Shimada Takashi, Yoshida Masaki, Ohnuma Mio, Fujiwara Takayuki, Imoto Yuuta, Yagisawa Fumi, Nishida Keiji, Hirooka Shunsuke, Misumi Osami, Mogi Yuko, Akakabe Yoshihiko, Matsushita Kazunobu, Kuroiwa Tsuneyoshi
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Journal Title
Proceedings of the National Academy of Sciences
Volume: 114
Issue: 50
Pages: 13284-13289
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Development of a double nuclear gene-targeting method by two-step transformation based on a newly established chloramphenicol-selection system in the red alga Cyanidioschyzon merolae2017
Author(s)
Fujiwara, T., Ohnuma, M., Kuroiwa, T., Ohbayashi, R., Hirooka, S.and Miyagishima, S.
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Journal Title
Front. Plant Sci.
Volume: 8
Pages: 343-343
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Book] Cyanidioschyzon merolae A New Model Eukaryote for Cell and Organelle Biology2017
Author(s)
Tsuneyoshi Kuroiwa, Shinya Miyagishima, Sachihiro Matsunaga, Naoki Sato, Hisayoshi Nozaki, Kan Tanaka, Osami Misumi, Shunsuke Hirooka, Yuuta Imoto, Yamato Yoshida, Fumi Yagisawa, Motomichi Matsuzaki, Masaki Yoshida, Takayuki Fujiwara, Mio Ohnuma, Haruko Kuroiwa, Mitsumasa Hanaoka, Nobuko Sumiya
Total Pages
365
Publisher
Springer
ISBN
9789811061004
Related Report
