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Genetic engineering study on increasing the production rate of algal biofuel using a novel culture system

Research Project

Project/Area Number 17K00676
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Design and evaluation of sustainable and environmental conscious system
Research InstitutionHiroshima National College of Maritime Technology

Principal Investigator

Ohnuma Mio  広島商船高等専門学校, その他部局等, 准教授 (70594076)

Co-Investigator(Kenkyū-buntansha) 三角 修己  山口大学, 大学院創成科学研究科, 准教授 (90583625)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords藻類バイオマス燃料 / 極限環境 / 単離 / オルガネラ / 油滴 / 細胞増殖 / 脂質生産 / ゲノム / 藻類バイオ燃料 / バイオマス利活用 / 藻類 / バイオ燃料
Outline of Final Research Achievements

Based on comprehensive gene expression analysis under lipid accumulation condition that does not inhibit cell proliferation using primitive eukaryotic red algae, Cyanidioschyzon merolae, RT-PCR analysis was demonstrated for four genes that were considered to be particularly important for lipid production and accumulation. RT-PCR analysis showed strong upregulation of these four genes. It was suggested that the gene is useful for increasing lipid production, and the overexpressing strain can be used for lipid production.
In parallel with the above study, we isolated a novel lipid-producing alga suitable for a low-cost mass-culture system from a high-pH hot spring. The alga belongs to the green alga Chlorococcum and produces lipids containing mainly nine kinds of fatty acids. Among the obtained fatty acids, the fatty acid with a particularly high content was octadecenoic acid as other lipid-producing algae.

Academic Significance and Societal Importance of the Research Achievements

高率的な藻類生産においては、脂質含有量が高く、増殖性の高い藻類が必須条件となる。ところが、一般の藻類では、細胞増殖と脂質の蓄積はトレードオフの関係があり、両立させることが困難である。シゾンを用いた遺伝子工学的研究により、細胞増殖をしながら脂質を蓄積する機構の基礎的知見が得られた。この知見は、広く藻類に適用できると考えられる。シゾンは培養温度40℃前後を好むため、当該遺伝子のシゾンの変異体は、脂質高生産株として、温泉地や工業施設などの近隣地域での藻類バイオ燃料生産に有用と考えられる。
並行して探索した新規脂質高生産藻類は、培養温度22℃前後を好むため、海洋などでの利用も視野に入れることができる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (15 results)

All 2019 2018 2017

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (10 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] Morphology, reproduction and taxonomy of Volvox dissipatrix (Chlorophyceae) from Thailand, with a description of Volvox zeikusii sp. nov.2019

    • Author(s)
      Nozaki Hisayoshi、Takusagawa Mari、Matsuzaki Ryo、Misumi Osami、Mahakham Wuttipong、Kawachi Masanobu
    • Journal Title

      Phycologia

      Volume: 58 Issue: 2 Pages: 192-199

    • DOI

      10.1080/00318884.2018.1540238

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Onsite GTP fuelling via DYNAMO1 drives division of mitochondria and peroxisomes2018

    • Author(s)
      Imoto Yuuta、Abe Yuichi、Honsho Masanori、Okumoto Kanji、Ohnuma Mio、Kuroiwa Haruko、Kuroiwa Tsuneyoshi、Fujiki Yukio
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 1-12

    • DOI

      10.1038/s41467-018-07009-z

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Glycosyltransferase MDR1 assembles a dividing ring for mitochondrial proliferation comprising polyglucan nanofilaments2017

    • Author(s)
      Yoshida Yamato, Kuroiwa Haruko, Shimada Takashi, Yoshida Masaki, Ohnuma Mio, Fujiwara Takayuki, Imoto Yuuta, Yagisawa Fumi, Nishida Keiji, Hirooka Shunsuke, Misumi Osami, Mogi Yuko, Akakabe Yoshihiko, Matsushita Kazunobu, Kuroiwa Tsuneyoshi
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 114 Issue: 50 Pages: 13284-13289

    • DOI

      10.1073/pnas.1715008114

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a double nuclear gene-targeting method by two-step transformation based on a newly established chloramphenicol-selection system in the red alga Cyanidioschyzon merolae2017

    • Author(s)
      Fujiwara, T., Ohnuma, M., Kuroiwa, T., Ohbayashi, R., Hirooka, S.and Miyagishima, S.
    • Journal Title

      Front. Plant Sci.

      Volume: 8 Pages: 343-343

    • DOI

      10.3389/fpls.2017.00343

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 単細胞紅藻 Cyanidioschyzon merolae のリン欠乏時の脂質合成遺伝子の研究2019

    • Author(s)
      大塚八雲,大沼みお,三角修己
    • Organizer
      日本植物形態学会 第31回大会(仙台)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 愛媛県鈍川温泉由来の油脂生産藻類とその脂肪滴2019

    • Author(s)
      大沼 みお,黒岩 晴子,黒岩 常祥,廣岡 俊亮,藤原 崇之,宮城島 進也,眞田 宣明,三角 修己
    • Organizer
      日本植物学会第83回大会(仙台)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 原始紅藻の硫黄濃度変動に対する適応応答機構の解析2019

    • Author(s)
      三角 修己,兼崎 友,齋藤 貴史,村上 瑞稀
    • Organizer
      日本植物学会第83回大会(仙台)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Algae sense exact temperatures: Analyses of small heat shock proteins at the survival threshold temperature in microalgae2019

    • Author(s)
      Osami Misumi
    • Organizer
      The International Symposium on Cellular Responses, Adaptation and Fermentation in Stress Environments (Yamaguchi)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 原始紅藻のバイオマス合成を誘導する外的要因と共通する代謝応答2019

    • Author(s)
      三角修己,斎藤貴史
    • Organizer
      日本藻類学会第43回大会 (京都)
    • Related Report
      2018 Research-status Report
  • [Presentation] 愛媛県鈍川温泉からの新油脂生産藻類の単離2018

    • Author(s)
      大沼みお, 黒岩晴子, 黒岩常祥, 三角修己
    • Organizer
      日本植物学会中国 四国支部大会(山口)
    • Related Report
      2018 Research-status Report
  • [Presentation] 愛媛県鈍川温泉から単離された新油脂生産藻類の脂肪滴2018

    • Author(s)
      大沼みお, 黒岩晴子, 黒岩常祥, 三角修己
    • Organizer
      日本植物形態学会 第30回大会(広島)
    • Related Report
      2018 Research-status Report
  • [Presentation] 愛媛県鈍川温泉から単離された新油脂生産藻類の脂肪滴2018

    • Author(s)
      大沼みお, 黒岩晴子, 黒岩常祥, 三角修己
    • Organizer
      日本植物学会第82回大会(広島)
    • Related Report
      2018 Research-status Report
  • [Presentation] 緑藻メダカモ(Medakamo hakoo)のオルガネラゲノムの特徴について2018

    • Author(s)
      三角修己,田草川真理,加藤翔一,乾弥生,松永幸大,黒岩晴子,黒岩常祥
    • Organizer
      日本植物学会第82回大会(広島)
    • Related Report
      2018 Research-status Report
  • [Presentation] 極小の微細藻類を用いた細胞の研究2018

    • Author(s)
      三角修己
    • Organizer
      日本細胞性粘菌学会第8回例会山口大会
    • Related Report
      2018 Research-status Report
  • [Book] Cyanidioschyzon merolae A New Model Eukaryote for Cell and Organelle Biology2017

    • Author(s)
      Tsuneyoshi Kuroiwa, Shinya Miyagishima, Sachihiro Matsunaga, Naoki Sato, Hisayoshi Nozaki, Kan Tanaka, Osami Misumi, Shunsuke Hirooka, Yuuta Imoto, Yamato Yoshida, Fumi Yagisawa, Motomichi Matsuzaki, Masaki Yoshida, Takayuki Fujiwara, Mio Ohnuma, Haruko Kuroiwa, Mitsumasa Hanaoka, Nobuko Sumiya
    • Total Pages
      365
    • Publisher
      Springer
    • ISBN
      9789811061004
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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