| Project/Area Number |
17K00852
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Eating habits
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| Research Institution | Mie University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | アルギン酸デオキシ糖 / DEH / KDG / DEHレダクターゼ / Formosa haliotis / Caenorhabditis elegans / 寿命延長効果 / ポリグルタミン凝集体 / ハンチントン病 / C. elegans N2 strain / TJ375 strain / HPLC / DEH還元酵素 / 寿命延長活性 / 抗酸化性 / 糖 / 酵素 / 応用微生物 / 生理活性 / バイオテクノロジー |
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| Outline of Final Research Achievements |
4-Deoxy-L-erythro-5-hexoseulose uronic acid (DEH) and 2-keto-3-deoxy-D-gluconic acid (KDG) are rare sugars produced from alginate by the action of lyase and reductase. In this study, we obtained sequence information of a novel DEH reductase gene from genomic DNA of alginate-utilizing bacteria, and developed a new method for producing KDG from DEH using the protein expression system in Escherichia coli. In addition, it was clarified that DEH has a physiological action of anti-aging effect, enhances stress tolerance and suppresses the progression of neurodegenerative diseases (polyglutamine diseases).
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| Academic Significance and Societal Importance of the Research Achievements |
DEHとKDGは，食用多糖類のアルギン酸から得られる希少糖である．DEHに加えKDGについても，1ステップの工程で純度約95%以上の産物を生産出来る方法を世界に先駆けて開発した．DEHは，生理機能としてアンチエイジング活性やストレス耐性の上昇効果，神経変異性疾患の進行を抑制する効果を持つことを明らかにした．したがって，DEHとKDG，この二つのアルギン酸デオキシ糖は，薬理効果を持つ新しい機能性単糖としての利用が期待される．
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