| Project/Area Number |
17K00903
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Eating habits
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
Baba Ryoko 産業医科大学, 医学部, 講師 (90271436)
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| Co-Investigator(Kenkyū-buntansha) |
國分 啓司 産業医科大学, 医学部, 助教 (00432740)
森本 景之 産業医科大学, 医学部, 教授 (30335806)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 小腸 / 回腸 / 吸収上皮細胞 / エンドサイトーシス / オルガノイド / 上皮 / パネート細胞 / 杯細胞 / 乳飲期 / 時間軸 |
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| Outline of Final Research Achievements |
In the neonatal mouse ileum, the absorptive cells that had an apical endocytic membrane system with a giant lysosome covered the surface of the villi. The system developed as the cells rise toward the tip of villus, and as mice grow. However, it disappeared in the absorptive cells after weaning. On the other hand, the shallow crypts formed around 2-weeks-old. Simultaneously, Paneth cells with many electron dense granules in the apical cytoplasm appeared in the base of the crypts. Its nucleus showed H3K27me positive.
In the organoid derived from neonatal mouse ileal crypt, the absorptive cells exhibited the similar structure to those of the mouse villi epithelium. H3K27me3 positive cells present in the organoid.
Our results suggested that epigenetic control participates in the shift the epithelial cell type from neonatal-suckling to weaning.
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| Academic Significance and Societal Importance of the Research Achievements |
典型的なパネート細胞が回腸の陰窩底部に出現する生後2週より前に、パネート細胞と杯細胞の中間型のような形態的特徴を示す複数段階の細胞（中間型細胞）が観察された。また、成熟期の陰窩において、深部ほど典型的なパネート細胞が観察され、より浅い部分に複数段階の中間型細胞が観察された。時間軸および絨毛-陰窩軸におけるパネート細胞及び中間型細胞の関連が明らかになった。近年、炎症性腸疾患等で、パネート細胞と杯細胞の中間型のような形態を示す細胞が出現することが多数報告されており、本研究成果を基に、今後、それらの疾患時に生じる細胞との関連について解明することで、それらの疾患の機序解明につながる可能性がある。
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