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Development of web-based learning materials using high-definition 3D reconstruction images and the application in life science education on the university course

Research Project

Project/Area Number 17K00981
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Science education
Research InstitutionKitasato University

Principal Investigator

Obata Shuichi  北里大学, 医療衛生学部, 准教授 (10204273)

Co-Investigator(Kenkyū-buntansha) 門谷 裕一  北里大学, 医療衛生学部, 教授 (10185887)
林 徹  北里大学, 医療衛生学部, 講師 (10454266)
木村 武俊  北里大学, 医療衛生学部, 助教 (80327444)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsデジタル教材 / 光学顕微鏡画像 / 立体再構築 / 科学教育 / 生命科学 / 動物胚 / 光学顕微鏡像 / 椎骨形成 / サーフィスレンダリング / セグメンテーション / 光学顕微鏡
Outline of Final Research Achievements

We established a method for development of web-based learning materials using high-definition 3D reconstruction images of vertebrate embryos and larvae, and investigated a better presentation method of them in a university education. A series of the established processes included fixation condition, resin for embedding, thickness of a section, effective collection of serial sections, optimum objective lens, image connection, batch image correction, software for making 3D reconstruction image. Using 1,000 serial sections (1 micrometer thickness of each section) of several stages of vertebrate embryos (or larvae) embedded in epoxy resin, we made high-definition 3D reconstruction images. Moreover, we considered several presentation method of 3D reconstruction images for the university students. The best method was to use movies which show the surface images by rotation and the inside structures by showing continuous changes from the surface to the inside at 3 axes.

Academic Significance and Societal Importance of the Research Achievements

本研究で確立した方法を用いて作成した高精細3D再構築画像は,複雑に変化する脊椎動物胚の形態形成過程を胚内部の構造も含めて大学生や大学院生に理解させるのに有効であることが分かった。この高精細3D再構築画像およびそれから作成した動画を,学生がいつでもアクセスできる状態でサーバー上に保存すれば,学生の学習時間の実質的増加につながる。さらに,この画像を一般公開できれば,今後その重要性が益々増加する生涯学習の教材としても利用できる。作成した3D再構築画像は光学顕微鏡レベルではあるものの高精細であるため,脊椎動物の椎骨形成過程の解明にも重要な役割を果たすことができた。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2020 2019 2018

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Novel erythrocyte clumps revealed by an orphan gene Newtic1 in circulating blood and regenerating limbs of the adult newt2018

    • Author(s)
      Casco-Robles Roman M., Watanabe Akihiko, Eto Ko, Takeshima Kazuhito, Obata Shuichi, Kinoshita Tsutomu, Ariizumi Takashi, nakatani Kei, Nakada Tomoaki, Tsonis Panagiotis A., Casco-Robles Martin M., Sakurai Keisuke, Yahata Kensuke, Maruo Fumiaki, Toyama Fubito, Chiba Chikafumi,
    • Journal Title

      Sci. Rep.

      Volume: 8 Issue: 1 Pages: 7455-7455

    • DOI

      10.1038/s41598-018-25867-x

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 原腸胚形成における細胞骨格の再構築による胚細胞運動の制御2020

    • Author(s)
      高野和敬,増本三香,小畑秀一,浅島 誠,永島雅文
    • Organizer
      第125回日本解剖学会総会・全国学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Ossification and morphogenesis of vertebra in Japanese newt, Cynops pyrrhogaster2019

    • Author(s)
      Ryunosuke Yanaka, Yuichi Kadoya, Shuichi Obata
    • Organizer
      The 19th Congress of the International Federation of Associations of Anatomists (IFAA 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アカハライモリの胚発生過程における椎骨形成2019

    • Author(s)
      谷中竜之介,門谷裕一,小畑秀一
    • Organizer
      日本動物学会第90回大阪大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 有尾両生類の胚発生過程における椎骨形成2019

    • Author(s)
      谷中竜之介、門谷裕一、小畑秀一
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 原腸胚形成における胚葉特異的な細胞運動の制御機構2019

    • Author(s)
      高野和敬、小畑秀一、増本三香、浅島 誠、永島雅文
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 胎仔マウス顎下腺の脱メチル化シトシンとDNA脱メチル化酵素の発現解析2019

    • Author(s)
      林 徹、門谷裕一
    • Organizer
      第124回日本解剖学会総会・全国学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 原腸胚形成における胚細胞の自律的な運動のしくみとその役割2018

    • Author(s)
      高野和敬,小畑秀一,増本三香,浅島 誠,永島雅文
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 胎仔マウス顎下腺の発生における脱メチル化制御機構2018

    • Author(s)
      林 徹,門谷裕一
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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