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Three Dimensional and co-culture model for Vascular metastasis of cancer

Research Project

Project/Area Number 17K01375
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionNihon University

Principal Investigator

Kataoka Noriyuki  日本大学, 工学部, 教授 (20250681)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsガン転移 / バイオメカニクス / 3次元共培養 / 3次元培養 / 骨組織 / 石灰化 / 血管内皮細胞 / 流れ負荷システム / アクチンフィラメント / ガン / 転移 / 血管 / 細胞培養
Outline of Final Research Achievements

Melanoma cells have metastasis-ability, invasive-ability, and migrate-ability. It is said that S100 protein triggers invasion of melanoma cells. In order to prevent metastasis of melanoma cells, it is important to know the migrate-ability of melanoma cells. Therefore, we constructed 3-dimensional culture model. In this study, in order to compare the influence on melanoma cells by using HEK 293, we made three conditions of models. And we analyzed the obtained data, behavior of melanoma cells were analyzed from two axes of X, Y and Z axes. We have successfully observe the cancer cell behavior on 3-dimensional culture model. Moreover, we have observed the cancer cell behavior on endothelial cells with fluid shear stress application. Under the aortic level of shear stress, cancer cell trans-endothelial migration were not observed.

Academic Significance and Societal Importance of the Research Achievements

現在のガン治療においては、早期診断・早期治療とともに、ガンの再発・転移の予防が重要な課題である。これまでの多くの研究により、分子レベルのガン細胞転移プロセスの解明は進んできた。ところが、各因子の相互的な作用、現象の連続的な解明は行われておらず、全容解明には程遠い状態である。このようななか、生化学的な研究と動物実験の相補的な役割を果たすのが、本研究で目指している「3次元共培養モデル」である。本研究のモデルを用いれば、分子、タンパク、細胞の挙動を実時間かつ3次元的にイメージングすることが可能であり、ガン細胞転移プロセスの解明の大きなブレイクスルーとなりうる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (7 results)

All 2019 2018 2017

All Presentation (7 results) (of which Int'l Joint Research: 2 results)

  • [Presentation] 骨芽細胞様株化細胞の石灰化及び分化に及ぼす長期力学刺激負荷の影響2019

    • Author(s)
      中嶋一雅、小松原和矩、長谷川翔大、下權谷祐児、片岡則之
    • Organizer
      日本機械学会 第30回バイオフロンティア講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 骨芽細胞様細胞に対する回転型流体せん断応力負荷に対する応答2019

    • Author(s)
      中嶋一雅、小松原和矩、下權谷祐児、片岡則之
    • Organizer
      日本機械学会 第32回バイオエンジニアリング講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Effect of mechanical stimulation on calcification and differentiation of MC3T3-E12019

    • Author(s)
      Kazunori Komatsubara, Shouta Hasegawa, Kazumasa Nakajima, Yuji shimogonya, Noriyuki Kataoka
    • Organizer
      10th Asian-Pacific Conference on Biomechanics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Real-time observation of F-actin fluctuation in living cell2018

    • Author(s)
      Tomoteru Oka, Gan Buntara Sthenly and Noriyuki Kataoka1
    • Organizer
      World Congress on Biomechanics 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 3 次元培養モデルを用いた流れ場でのメラノーマ細胞の実時間観察2018

    • Author(s)
      斎藤雅史、菅野健太、霜田浩貴、竹下彪我、堤浩平、片岡則之
    • Organizer
      日本機械学会 第31回バイオエンジニアリング講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] 生細胞内アクチンフィラメントの微小ゆらぎの計測2017

    • Author(s)
      岡 知輝, 片岡則之
    • Organizer
      第56回日本生体医工学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 生細胞アクチンフィラメントの微小ゆらぎの解析2017

    • Author(s)
      岡 知輝, 片岡則之
    • Organizer
      第28回バイオフロンティア講演会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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