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Development of intracellular multi-monitoring system and its application for analysis of glycation effect on tumor cells

Research Project

Project/Area Number 17K01376
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionTokyo University of Technology

Principal Investigator

SAKAGUCHI-MIKAMI Akane  東京工科大学, 医療保健学部, 講師 (70469782)

Co-Investigator(Kenkyū-buntansha) 山崎 智彦  国立研究開発法人物質・材料研究機構, 機能性材料研究拠点, 主幹研究員 (50419264)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords生体情報・計測 / 細胞内計測 / 糖化関連疾病 / 細胞内糖化 / 腫瘍細胞 / 糖化物
Outline of Final Research Achievements

In this study we attempted to investigate intracellular glycation in tumor cells and its effect on cell functions using intracellular glycation monitoring system, prior to elucidation of involvement mechanism for intracellular glycation on tumor progression.
The result showed that the intracellular glycation level was not change in myeloma cells whereas significantly increased in aged-model tumor cells with the increase of medium glucose. The glucose metabolism and gene expression patterns of the cells suggested that the glycation level in myeloma cells may be controlled by a mechanism other than glucose metabolism system. It is also indicated that genes concerned with intracellular glycation level in each tumor cells are different. Further studies to identify responsibility genes for intracellular glycation level and their cellular functions are expected to contribute to the elucidation of involvement mechanism for intracellular glycation on tumor progression.

Academic Significance and Societal Importance of the Research Achievements

本研究では,近年,細胞内糖化と脱糖化のバランスが細胞機能及びエピジェネティック制御に影響し,がんや加齢関連疾患の進行などの発症・進行に関与するとして注目されている細胞内糖化レベルの簡便なモニタリングを可能とする技術を開発した.また本技術を用いた腫瘍細胞内糖化モニタリングにより,細胞内糖化制御に関与する因子が腫瘍細胞ごとに異なっていることが示唆された.本技術を用いた腫瘍細胞内糖化物の解析は、腫瘍進行と糖化の関与機構の解明のみならず、バイオマーカーの開発や薬剤スクリーニングにも有用であり、がんの治療及び早期発見に貢献すると期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (5 results)

All 2020 2019 2017

All Presentation (5 results)

  • [Presentation] A Novel Monitoring System for Intracellular glycation2020

    • Author(s)
      Akane Sakaguchi-Mikami, Maho Sakurai, Shoya Kojima, Yukimi Sugimoto, Misato Kashiba
    • Organizer
      日本化学会第100回春季年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 土壌細菌由来新規糖化産物応答性転写調節因子2019

    • Author(s)
      三上あかね、Yani Faozani Alli
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 新規糖化タンパク質応答性転写調節因子の検索2017

    • Author(s)
      金村孝太、綾野晃一、三上あかね
    • Organizer
      第27回日本メイラー ド学会(女子栄養大学 埼玉)
    • Related Report
      2017 Research-status Report
  • [Presentation] 細胞糖化マルチモニタリング用蛍光モニタリング法の開発2017

    • Author(s)
      相上莉穂、廣原まゆ、三上あかね
    • Organizer
      第27回日本メイラー ド学会(女子栄養大学 埼玉)
    • Related Report
      2017 Research-status Report
  • [Presentation] 新規糖化物応答転写調節因子に基づく細胞内糖化物モ ニタリング法の開発2017

    • Author(s)
      金井知芙優、内田直恵、アリ ヤニ・ファオザニ、三上あかね
    • Organizer
      第27回日本メイラー ド学会(女子栄養大学 埼玉)
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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