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Construction of three-dimensional hard tissue with vascular network using a novel fusion process

Research Project

Project/Area Number 17K01396
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionMeiji University

Principal Investigator

Honda Michiyo  明治大学, 理工学部, 専任准教授 (20384175)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords骨再生 / 血管新生 / バイオマテリアル / スキャフォルド / 血管ネットワーク / 硬組織再生
Outline of Final Research Achievements

The aim of this study is to construct bone-like tissue with three-dimensional structure by combination with biocompatible scaffold and periosteum-like sheet consisting of vascular endothelial cells and osteoblasts. The angiogenic proteins-loaded materials could enhance the cellular attachment through interaction with integrin and promote actin cytoskeletal reorganization. In the case of co-culture of osteoblasts and endothelial cells on a microfiber biomaterial, osteoblasts and endothelial cells could enhance and improve each other’s function such as osteoblastic differentiation and endothelial cell tube formation. These results were supported by the properties of the scaffold such as the fibrous and topological structures. This approach can provide the evidences for the effectiveness of the tissue-engineered bone with vascular networks by crosstalk between osteoblasts and endothelial cells and interaction between these cells and the scaffold.

Academic Significance and Societal Importance of the Research Achievements

超高齢社会を迎えた我が国において、骨組織をはじめとする生体組織の再生はiPS細胞を含む幹細胞による生体の構築に大きく貢献することができる。将来的にはそれらの技術は国民の生活の質の向上に寄与することが期待できる。本研究では、組織再生において重要と位置付けられる血管構造を有する骨膜様組織の構築に成功した。ネットワーク構造を有する足場材料は、再生組織を構築する細胞と血管形成に必要な細胞が相互作用し得る環境を提供し、血管網を備えた機能的で移植可能なサイズを持つ骨膜様組織の作出を実現した。この技術は、骨折などを含む硬組織の再生に大きく貢献し得ると考える。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (21 results)

All 2019 2018 2017

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 4 results) Presentation (10 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results) Book (2 results)

  • [Journal Article] Co-Culture of Osteoblasts and Endothelial Cells on a Microfiber Scaffold to Construct Bone-Like Tissue with Vascular Networks2019

    • Author(s)
      Inomata Kouki、Honda Michiyo
    • Journal Title

      Materials

      Volume: 12 Issue: 18 Pages: 2869-2869

    • DOI

      10.3390/ma12182869

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acceleration of Osteogenesis via Stimulation of Angiogenesis by Combination with Scaffold and Connective Tissue Growth Factor2019

    • Author(s)
      Honda Michiyo、Hariya Ryo、Matsumoto Morio、Aizawa Mamoru
    • Journal Title

      Materials

      Volume: 12 Issue: 13 Pages: 2068-2068

    • DOI

      10.3390/ma12132068

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inhibitory effects of zoledronic acid-loaded bioresorbable carrier on osteosarcoma2018

    • Author(s)
      M. Honda, N. Suzuki, K. Nagata, M. Aizawa
    • Journal Title

      Key. Eng. Mater.

      Volume: 782 Pages: 297-302

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] In vivo evaluation of porous hydroxyapatite ceramics including bone minerals using pig model2018

    • Author(s)
      Yokota Tomohiro、Nakano Kazuaki、Nagaya Masaki、Honda Michiyo、Nagashima Hiroshi、Aizawa Mamoru
    • Journal Title

      Materials Technology

      Volume: 33 Issue: 10 Pages: 689-697

    • DOI

      10.1080/10667857.2018.1495392

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fabrication and biological evaluation of hydroxyapatite ceramics including bone minerals2018

    • Author(s)
      Tomohiro Yokota, Takuya Miki, Michiyo Honda, Tomoko Ikeda-Fukazawa, Ken Ishii, Morio Matsumoto, and Mamoru Aizawa
    • Journal Title

      Journal of the Ceramic Society of Japan

      Volume: 126 Issue: 2 Pages: 99-108

    • DOI

      10.2109/jcersj2.17216

    • NAID

      130006329675

    • ISSN
      1348-6535, 1882-0743
    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bioresorbability of chelate-setting calcium-phosphate cement hybridized with gelatin particles using a porcine tibial defect model2018

    • Author(s)
      KIMINAMI Keishi、NAGATA Kohei、KONISHI Toshiisa、MIZUMOTO Minori、HONDA Michiyo、NAKANO Kazuaki、NAGAYA Masaki、ARIMURA Hidetoshi、NAGASHIMA Hiroshi、AIZAWA Mamoru
    • Journal Title

      Journal of the Ceramic Society of Japan

      Volume: 126 Issue: 2 Pages: 71-78

    • DOI

      10.2109/jcersj2.17197

    • NAID

      130006329683

    • ISSN
      1348-6535, 1882-0743
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inhibition of bacterial adhesion using calcium phosphate microspheres loaded with protamine2017

    • Author(s)
      M. Honda, Y. Kawanobe, H. Uchida, M. aizawa
    • Journal Title

      Int. J. Metall. Mater. Eng.

      Volume: 3 Issue: 2 Pages: 1-5

    • DOI

      10.15344/2455-2372/2017/136

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Preliminary study for Co-Culture of Osteoblasts and Endothelial Cells to Construct the Regenerative Bone2017

    • Author(s)
      M. Honda, M. Aizawa
    • Journal Title

      Key. Eng. Mater.

      Volume: 758 Pages: 269-272

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fabrication of chelate-setting α-tricalcium phosphate cement using sodium citrate and sodium alginate as mixing solution and its in vivo osteoconductivity2017

    • Author(s)
      T. Konishi, PN. Lim, M. Honda, M. Nagaya, H. Nagashima, ES. Thian, M. Aizawa
    • Journal Title

      J. Biomed. Mater. Res. B

      Volume: 17 Issue: 6 Pages: 1-10

    • DOI

      10.1002/jbm.b.34028

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 温度変化に伴う骨分化及び血管新生への影響2019

    • Author(s)
      猪俣光稀、本田みちよ
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 血管内皮細胞との共培養による歯根膜線維芽細胞の分化への影響2019

    • Author(s)
      結城大悟、本田みちよ
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 骨芽細胞構造体の作製とその機能性評価2019

    • Author(s)
      片桐奈津子、坂井秀昭、本田みちよ
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Inhibitory effects of zoledronic acid-loaded bioresorbable cement on osteosarcoma2018

    • Author(s)
      M. Honda, N. Suzuki, K. Nagata, M. Aizawa
    • Organizer
      Bioceramics30
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Evaluation of antimicrobial activity of protamine against Cutibacterium acnes2018

    • Author(s)
      Y. Tashiro, M. Aizawa, M. Honda
    • Organizer
      The 18th Asian bioceramics symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Zinc induces bone differentiation through Hedgehog-Gli Signaling Pathway2018

    • Author(s)
      Iwao Sato, Mamoru Aizawa, Michiyo Honda
    • Organizer
      The 18th Asian bioceramics symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 亜鉛のHedgehog-Gli経路を介した骨髄間質細胞の骨分化促進効果2018

    • Author(s)
      佐藤巌,本田みちよ
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Three-dimensional scaffolds for bone tissue engineering2017

    • Author(s)
      M. Honda, and M. Aizawa,
    • Organizer
      STAC10
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Acceleration of osteogenesis and angiogenesis by CTGF-containing apatite-fiber scaffold2017

    • Author(s)
      M. Honda, K. Nagata, T. Yokota, K. Ishii, M. Natsumoto, and M. Aizawa
    • Organizer
      IUMRS-ICAM
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Promotion of osteogenesis by combination of apatite-fiber scaffold and connective tissue growth factor2017

    • Author(s)
      M. Honda, and M. Aizawa
    • Organizer
      Bioceramics29
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Book] 動物細胞培養・自動化におけるトラブル発生原因と対策2017

    • Author(s)
      本田みちよ,相澤 守
    • Total Pages
      526
    • Publisher
      技術情報協会
    • Related Report
      2017 Research-status Report
  • [Book] Advances in Bioinspired and Biomedical Materials2017

    • Author(s)
      M. Aizawa, M. Honda, M. Emoto,
    • Total Pages
      303
    • Publisher
      ACS Books
    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

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