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Development of Microfluidic Devices for Circulating Tumor Cell Separation

Research Project

Project/Area Number 17K01432
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Medical systems
Research InstitutionToyama Industrial Technology Research and Development Center

Principal Investigator

TAKATA Koji  富山県産業技術研究開発センター, その他部局等, 主任研究員 (40530621)

Co-Investigator(Kenkyū-buntansha) 横堀 武彦  群馬大学, 未来先端研究機構, 准教授 (60420098)
安田 佳織  富山県立大学, 工学部, 講師 (70707231)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords循環がん細胞 / がん / マイクロ流路チップ / 低侵襲治療システム / 癌
Outline of Final Research Achievements

Circulating tumor cells (CTC) are tumor-derived cells circulating in bloodstream and could be the origin of lethal metastatic disease. Easy-to-use and high-throughput methods for CTC separation have potential to be used in innovative cancer therapy, next-generation diagnosis, personalized medicine, fundamental metastasis research, etc. In this study, we newly developed a microfluidic device which could easily separate CTCs, and devices which could be used for high-throughput cell separation. These devices were tested using blood sample spiked with cultured cancer cells, and we showed that the devices could successfully separate cultered cancer cells.

Academic Significance and Societal Importance of the Research Achievements

これまで開発してきたマイクロ流路チップをベースに、簡便に、かつ、大量の血液からCTCを分離する方法を開発する研究である。大量の血液からCTCが分離できるようになれば、転移を抑える革新的ながん治療等に繋がると考えられる。また、血液からCTCを分離・回収することもできるため、これまでCTCが希少なため困難であったCTCによる癌の状態のモニタリング、最適な治療法の選択等にも用いることができ、がん医療に多大な貢献ができると考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (6 results)

All 2020 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results)

  • [Journal Article] High expression of carcinoembryonic antigen and telomerase reverse transcriptase in circulating tumor cells is associated with poor clinical response to the immune checkpoint inhibitor nivolumab2018

    • Author(s)
      Bao H、Bai T、Takata K、Yokobori T、Ohnaga T、Hisada T、Maeno T、Bao P、Yoshida T、Kumakura Y、Honjo H、Sakai M、Sohda M、Fukuchi M、Altan B、Handa T、Ide M、Miyazaki T、Ogata K、Oyama T、Shimizu K、Mogi A、Asao T、Shirabe K、Kuwano H、Kaira K
    • Journal Title

      Oncology Letters

      Volume: 15 Pages: 3061-3067

    • DOI

      10.3892/ol.2017.7671

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] マイクロ流体チップによるCTC(circulating tumor cell)の検出2020

    • Author(s)
      松村吉晃、高田耕児、久米健一、別府真広、山下麻由美、東友太郎、杉浦剛
    • Organizer
      第4回Liquid Biopsy研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 循環腫瘍細胞分離におけるDeterministic Lateral Displacement(DLD)法を用いたサイズ分離チップの基礎検討2020

    • Author(s)
      森將鷹、金山雅俊、桒田泰治、米田和恵、高田耕児、田中文啓
    • Organizer
      第4回Liquid Biopsy研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 低コストCTC分離装置2018

    • Author(s)
      高田耕児
    • Organizer
      日本癌学会学術総会
    • Related Report
      2018 Research-status Report
  • [Presentation] SS-Chipを用いたCTCの検出2018

    • Author(s)
      松村吉晃、高田耕児、久米健一、別府真広、山下麻由美、杉浦剛
    • Organizer
      日本癌学会学術総会
    • Related Report
      2018 Research-status Report
  • [Presentation] CTCを利用したニボルマブ感受性マーカーの探索研究2018

    • Author(s)
      横堀武彦、高田耕児、浅尾高行、清水公裕、茂木晃、解良恭一、調憲、桑野博行
    • Organizer
      癌免疫外科研究会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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