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Development of muscle atrophy prevention method using human skeletal muscle cells

Research Project

Project/Area Number 17K01485
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Rehabilitation science/Welfare engineering
Research InstitutionFujita Health University

Principal Investigator

Nakatani Masashi  藤田医科大学, 総合医科学研究所, 助教 (00421264)

Co-Investigator(Kenkyū-buntansha) 中井 滋  藤田医科大学, 保健学研究科, 教授 (20345896)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords骨格筋 / 筋衛星細胞 / 間葉系前駆細胞 / 異所性骨化 / 分子スクリーニング / 筋肉内異所性骨化 / ヒト骨格筋由来細胞 / サルコペニア / マイオスタチン / ヒト骨格筋由来筋芽細胞
Outline of Final Research Achievements

Human satellite cells and mesenchymal progenitor cells derived from human skeletal muscle were isolated and used as research materials. Since the originally planned muscle satellite cells took time to isolate a highly differentiated strain. Therefore, screening using mesenchymal progenitor cells was performed.As a result, one candidate molecule derived from a natural component could be identified.We plan to continue in vivo analysis in the future.To identify the skeletal muscle atrophy preventive factor, sera of long-term dialysis patients were collected and patients with muscle atrophy were identified based on the amount of creatinine. In the future, we plan to identify the factors involved in muscle atrophy using the serum of this patient.

Academic Significance and Societal Importance of the Research Achievements

本研究は、ヒト骨格筋から筋の恒常性に関わっている、2つの細胞(筋衛星細胞、間葉系前駆細胞)を単離し、その細胞を用いて有益な化合物・薬剤の探索を行う。ヒト骨格筋から単離した細胞を用いることで、ヒトの病態に直結し、有効な化合物を同定できる可能性が高い。本研究で作用が明らかになった分子は天然由来の経口で摂取されている分子であるため、筋の恒常性を維持する新しい化合物としての可能性が高い。近年の高齢化に伴う社会において、骨格筋の恒常性の維持は非常に重要であるため、本研究結果は社会的意義が大きいと考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2020 2019 2018 2017

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 5 results) Presentation (2 results)

  • [Journal Article] Expression Levels of Long Non-Coding RNAs Change in Models of Altered Muscle Activity and Muscle Mass2020

    • Author(s)
      Hitachi Keisuke、Nakatani Masashi、Funasaki Shiori、Hijikata Ikumi、Maekawa Mizuki、Honda Masahiko、Tsuchida Kunihiro
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 5 Pages: 1628-1628

    • DOI

      10.3390/ijms21051628

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Desloratadine inhibits heterotopic ossification by suppression of BMP2‐Smad1/5/8 signaling2020

    • Author(s)
      Kusano Taiki、Nakatani Masashi、Ishiguro Naoki、Ohno Kinji、Yamamoto Naoki、Morita Mitsuhiro、Yamada Harumoto、Uezumi Akiyoshi、Tsuchida Kunihiro
    • Journal Title

      Journal of Orthopaedic Research

      Volume: in press Issue: 6 Pages: 11-11

    • DOI

      10.1002/jor.24625

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Long Non-Coding RNA Myoparr Regulates GDF5 Expression in Denervated Mouse Skeletal Muscle.2019

    • Author(s)
      Hitachi K, Nakatani M, Tsuchida K.
    • Journal Title

      Noncoding RNA

      Volume: 5 Issue: 2 Pages: 33-33

    • DOI

      10.3390/ncrna5020033

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reduced expression of calcitonin receptor is closely associated with age-related loss of the muscle stem cell pool.2019

    • Author(s)
      Ikemoto-Uezumi M, Uezumi A, Zhang L, Zhou H, Hashimoto M, Okamura K, Matsui Y, Tsukazaki K, Hosoyama T, Nakatani M, Morita M, Yamada H, Tsuchida K, Fukada S.
    • Journal Title

      JCSM Rapid Communications

      Volume: 2

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Myogenin promoter-associated lncRNA Myoparr is essential for myogenic differentiation.2019

    • Author(s)
      Hitachi K, Nakatani M, Takasaki A, Ouchi Y, Uezumi A, Ageta H, Inagaki H, Kurahashi H, Tsuchida K.
    • Journal Title

      EMBO Reports

      Volume: 20 Issue: 3 Pages: 47468-47468

    • DOI

      10.15252/embr.201847468

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] UBL3 modification influences protein sorting to small extracellular vesicles.2018

    • Author(s)
      Ageta H, Ageta-Ishihara N, Hitachi K, Karayel O, Onouchi T, Yamaguchi H, Kahyo T, Hatanaka K, Ikegami K, Yoshioka Y, Nakamura K, Kosaka N, Nakatani M, Uezumi A, Ide T, Tsutsumi Y, Sugimura H, Kinoshita M, Ochiya T, Mann M, Setou M, Tsuchida K.
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 3936-3936

    • DOI

      10.1038/s41467-018-06197-y

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Promethazine hydrochloride inhibits ectopic fat cell formation in skeletal musce.2017

    • Author(s)
      Kasai T, Tsuchida, K. et al.,
    • Journal Title

      Am. J. Pathol.

      Volume: 187 Issue: 12 Pages: 2627-2634

    • DOI

      10.1016/j.ajpath.2017.08.008

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ヒト骨格筋由来細胞を用いた筋量調節機構の解明2019

    • Author(s)
      中谷直史、中井滋、大橋篤、土田邦博
    • Organizer
      日本肥満学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ヒト骨格筋由来細胞を用いた分子スクリーニング2018

    • Author(s)
      中谷 直史、山本 直樹、森田 充浩,、山田 治基、土田 邦博
    • Organizer
      日本肥満学会
    • Related Report
      2018 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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