the regulatory mechanism of muscle stem cells with exosomes

• Project/Area Number: 17K01859
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: Fujita Health University (2018-2019); Kyoto Prefectural University of Medicine (2017)
• Principal Investigator: Sato Takahiko 藤田医科大学, 医学部, 講師 (30570775)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: エクソソーム / 骨格筋幹細胞 / 細胞外小胞 / 発生・分化

Outline of Final Research Achievements

We have identified the genes that are significantly changed in differentiated myocytes with or without exosomes of serum, and focused on Tceal family.
Tceal7 expression is up-regulated in differentiated myotubes, and is detected in somites of developing mouse embryos.

Academic Significance and Societal Importance of the Research Achievements

骨格筋研究において老化時の影響を調査したものは数多く存在するが、幹細胞という点から研究を進めているものは実は曖昧なものが多い。加齢による転写後の遺伝子発現制御機構、そしてエクソソーム小胞をはじめとする細胞間相互作用による関係を知る事により、骨格筋幹細胞から骨格筋分化を決定する機構の全体像、組織幹細胞の未分化性維持の為の機構解明、そして骨格筋再生時の分子機構解明に役立つと考えている。

Research Products

• [Journal Article] Core Transcription Factors Promote Induction of PAX3-Positive Skeletal Muscle Stem Cells (2019)
  • Author(s): Sato Takahiko、Higashioka Koki、Sakurai Hidetoshi、Yamamoto Takuya、Goshima Naoki、Ueno Morio、Sotozono Chie
  • Journal Title: Stem Cell Reports Volume: 13 Issue: 2 Pages: 352-365
  • DOI: 10.1016/j.stemcr.2019.06.006
  • NAID: 120006843488
  • Peer Reviewed / Open Access
• [Journal Article] Human Skeletal Muscle Cells Derived from the Orbicularis Oculi Have Regenerative Capacity for Duchenne Muscular Dystrophy (2019)
  • Author(s): Yamanaka Yukito、Takenaka Nana、Sakurai Hidetoshi、Ueno Morio、Kinoshita Shigeru、Sotozono Chie、Sato Takahiko
  • Journal Title: International Journal of Molecular Sciences Volume: 20 Issue: 14 Pages: 3456-3456
  • DOI: 10.3390/ijms20143456
  • Peer Reviewed / Open Access
• [Presentation] 標的遺伝子欠損iPS細胞を用いた神経筋疾患解析 (2019)
  • Author(s): 佐藤 貴彦、平坂 勝也、二川 健、内田 貴之
  • Organizer: 第42回日本分子生物学会
• [Presentation] Core transcription factors promote induction from human iPS cells of functional Pax3-positive muscle stem cells (2018)
  • Author(s): Sato Takahiko
  • Organizer: 第51回日本発生生物学会
• [Presentation] 主要転写因子を用いた骨格筋幹細胞への誘導研究 (2018)
  • Author(s): 佐藤貴彦
  • Organizer: 第９１回日本組織培養学会
• [Presentation] Core transcription factors promote induction of functional Pax3-positive muscle stem cells (2018)
  • Author(s): Sato Takahiko
  • Organizer: 5th TERMIS World Congress
  • Int'l Joint Research