| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Outline of Final Research Achievements |
In this study, we aimed to quantitatively analyze the effects of topological changes in DNA quadruplex structures on gene replication reactions and to elucidate their intracellular functions. For this purpose, we developed a correlation analysis method between the energy value of the nucleic acid structure and the replication rate, and quantitatively analyzed the gene replication control mechanism involving the nucleic acid topology. It was found that the replication inhibition effect was different depending on the quadruplex topology. Furthermore, by using pyrene-modifieded artificial nucleic acids, we succeeded in changing the topology of the G-quadruplex of the oxidatively damaged VEGF gene and restored the regulatory function of the replication reaction that was impaired by oxidative damage.
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