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Differentiation Induction of Human Mesenchymal Stem Cells by Graphene Attached Hard/Soft Hybrid Structure Substrates

Research Project

Project/Area Number 17K05009
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Nanomaterials engineering
Research InstitutionNippon Institute of Technology

Principal Investigator

Ban Masahito  日本工業大学, 基幹工学部, 教授 (70424059)

Co-Investigator(Kenkyū-buntansha) 沖川 侑揮  国立研究開発法人産業技術総合研究所, 材料・化学領域, 研究員 (50635315)
石原 正統  国立研究開発法人産業技術総合研究所, 材料・化学領域, 主任研究員 (70356450)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsグラフェン / 幹細胞 / 増殖 / 分化 / 神経 / ナノ材料 / 細胞足場
Outline of Final Research Achievements

Hard/soft hybrid structure substrates attached graphene with discriminating properties, extremely thin sheet-like structure, high elastic modulus and electric conductivity, were fabricated, and using these as the scaffolds culture tests of human mesenchymal stem cells were conducted. The substrates were obtained by fractionating the attached graphene into fine domains by means of applying a tensile strain. It was found that the graphene had a profound effect on promotion of cell proliferation at various conditions, substrates with different modulus, and basic medium and neuronal differentiation induction medium. Graphene did not indicate a significant effect on induction of differentiation into neuronal cells.

Academic Significance and Societal Importance of the Research Achievements

細胞足場として使用する基質の表面にグラフェンを付着させることで、ヒト間葉系幹細胞を大幅に増殖させることできることが明らかになった。この効果は、基質が軟質なPDMSだけでなくガラス基板においても、また、基本培地のほか分化誘導培地を使用した場合においても認められた。このことは、グラフェンが、ヒト間葉系幹細胞細胞の高効率な増殖用足場として使用できる可能性を示しており、培養時間の短縮を図るための有力なツールになることが期待できる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (32 results)

All 2020 2019 2018 2017

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (27 results) (of which Int'l Joint Research: 6 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] Extracting Carrier Mobility Using a Photoinduced Charge Transfer Reaction: From conducting polymers to nanocarbon materials2020

    • Author(s)
      Lingyun Lyu, Kazuhiro Kirihara, Yuki Okigawa, Masataka Hasegawa, Wuxiao Ding, Ying Wang, Masakazu Mukaida, Ying Zhou, Qingshuo Wei
    • Journal Title

      Organic Electronics

      Volume: 78 Pages: 105615-105615

    • DOI

      10.1016/j.orgel.2020.105615

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] グラフェン2020

    • Author(s)
      石原正統、水谷亘、山田貴壽、沖川侑揮、長谷川雅考
    • Journal Title

      表面技術

      Volume: 71 Pages: 189-189

    • NAID

      130007883207

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Fabrication of Arrayed Microwells with Wrinkle Microstructure by Ink-jet and Diamond-like Carbon Thin Film Deposition Process2019

    • Author(s)
      Masahito Ban, Yuuya Kogi, Taichi Hirose
    • Journal Title

      Materials Science and Engineering B

      Volume: 249 Pages: 114422-114422

    • DOI

      10.1016/j.mseb.2019.114422

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of diamond-like carbon thin film and wrinkle microstructure on cell proliferation2018

    • Author(s)
      Ban Masahito、Tobe Satoshi、Takeuchi Louie
    • Journal Title

      Diamond and Related Materials

      Volume: 90 Pages: 194-201

    • DOI

      10.1016/j.diamond.2018.10.021

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] DLC薄膜を用いたナノプラスチックの神経細胞毒性評価2020

    • Author(s)
      伴雅人、下田亮太
    • Organizer
      表面技術協会第141回講演大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] カーボン系薄膜パターニング基板の作製2020

    • Author(s)
      下田亮太、石原正統、沖川侑揮、伴雅人
    • Organizer
      表面技術協会第141回講演大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Behaviors of Mesenchymal Stem Cells on Graphene Attached Soft Material Structure2019

    • Author(s)
      Masahito Ban, Ryouta Shimoda, Masatou Ishihara, Yuki Okigawa
    • Organizer
      Tissue Engineering and Regenerative Medicine International Society - AP Chapter and the 7th Asian Biomaterials Congress (TERMIS-AP + ABMC7 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DLC薄膜成膜微細凹凸構造を用いた間葉系幹細胞の培養における効果2019

    • Author(s)
      伴雅人、竹上優
    • Organizer
      第39回化学とマイクロ・ナノシステム研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] DLC成膜微細凹凸構造による神経芽細胞腫の培養2019

    • Author(s)
      伴雅人、陳静
    • Organizer
      表面技術協会第140回講演大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 断片化したCVDグラフェン転写PDMS基板によるヒト間葉系幹細胞の培養評価2019

    • Author(s)
      下田亮太、石原正統、沖川侑揮、伴 雅人
    • Organizer
      表面技術協会第140回講演大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ダイヤモンドライクカーボン(DLC)薄膜の細胞足場への応用2019

    • Author(s)
      伴雅人
    • Organizer
      29th Annual Meeting of MRS-Japan
    • Related Report
      2019 Annual Research Report
  • [Presentation] グラフェン付着ソフトマテリアルの細胞増殖への効果2019

    • Author(s)
      下田亮太、石原正統、沖川侑揮、伴 雅人
    • Organizer
      29thAnnual Meeting of MRS-Japan
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高品質プラズマCVDグラフェン2019

    • Author(s)
      石原正統、沖川侑揮、伴雅人、長谷川雅考
    • Organizer
      29th Annual Meeting of MRS-Japan
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] CVDグラフェンによる ヒト間葉系幹細胞の増殖2019

    • Author(s)
      下田亮太、石原正統、沖川侑揮、伴雅人
    • Organizer
      グラフェン・酸化グラフェン合同シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] Thermoelectric Characteristics of Graphene for Recovery of Low Temperature Waste Heat2019

    • Author(s)
      J. Kihara, M. Ishihara, Y. Okigawa, S. Koura
    • Organizer
      Thermoelectric Characteristic
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Relationship between Mobility and Raman Spectra for Plasma CVD Graphene on HTHP h-BN2019

    • Author(s)
      Y. Okigawa, T. Yamada, M. Hasegawa, K. Watanabe, T. Taniguchi
    • Organizer
      Recent Progress in Graphene and Two-dimensional Materials Research Conference (RPGR2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dry Transfer of Chemical Vapor Deposition Graphene onto Silicon Wafers Treated by Silan Coupling Agents2019

    • Author(s)
      M. Ishihara, M. Hasegawa
    • Organizer
      European Graphene Forum 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ラマン分光法を用いたCVDグラフェンの移動度散乱機構の解明2019

    • Author(s)
      沖川侑揮、谷口尚、渡邊賢司、長谷川雅考、山田貴壽
    • Organizer
      第33回ダイヤモンドシンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] アミノシランで処理したシリコンウェハへのCVDグラフェンの転写と評価2019

    • Author(s)
      石原正統、長谷川雅考
    • Organizer
      表面技術協会第140回講演大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] DLC成膜微細凹凸構造によるヒト間葉系幹細胞の分化2019

    • Author(s)
      伴雅人、竹上優
    • Organizer
      表面技術協会第139回講演大会
    • Related Report
      2018 Research-status Report
  • [Presentation] CVDグラフェン転写PDMS基板によるヒト間葉系幹細胞の分化2019

    • Author(s)
      下田亮太、石原正統、沖川侑揮、長谷川雅考、伴雅人
    • Organizer
      表面技術協会第139回講演大会
    • Related Report
      2018 Research-status Report
  • [Presentation] パターニング成膜されたDLC薄膜によるヒト間葉系幹細胞の挙動制御2019

    • Author(s)
      竹上優、伴雅人
    • Organizer
      表面技術協会第139回講演大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Stem Cell Behavior Control by Surface Wave Plasma CVD Graphene Attached on PDMS2018

    • Author(s)
      Masahito Ban, Masatou Ishihara, Yuki Okigawa, Masataka Hasegawa
    • Organizer
      International Conference on Diamond and Carbon Materials
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] DLC薄膜成膜による微細凹凸構造のヒト間葉系幹細胞の挙動への効果2018

    • Author(s)
      伴雅人、戸辺悟司
    • Organizer
      表面技術協会第138回講演大会
    • Related Report
      2018 Research-status Report
  • [Presentation] CVDグラフェン転写PDMS基板によるヒト間葉系幹細胞の培養2018

    • Author(s)
      伴雅人、下田亮太、石原正統、沖川侑揮、長谷川雅考
    • Organizer
      第40回日本バイオマテリアル学会シンポジウム
    • Related Report
      2018 Research-status Report
  • [Presentation] カーボン系薄膜を用いた細胞足場の開発2018

    • Author(s)
      伴雅人
    • Organizer
      表面技術協会関東支部講演会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] CVDグラフェン転写基板によるヒト間葉系幹細胞の培養評価2018

    • Author(s)
      伴雅人、吉澤利哉、石原正統、沖川侑揮、長谷川雅考
    • Organizer
      表面技術協会第137回講演大会
    • Related Report
      2017 Research-status Report
  • [Presentation] DLC薄膜成膜による微細凹凸構造の寸法と細胞増殖の関係2018

    • Author(s)
      伴雅人、戸辺悟司、竹内瑠維
    • Organizer
      表面技術協会第137回講演大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Effect of diamond-like carbon thin film and wrinkle microstructure on cellular behavior2017

    • Author(s)
      Masahito Ban, Louie Takeuchi, Ryuki Arayama
    • Organizer
      28th European Conference on Biomaterials
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] DLC薄膜および微細凹凸構造の培養細胞への効果 その22017

    • Author(s)
      竹内瑠維、戸辺悟司、伴雅人
    • Organizer
      表面技術協会第136回講演大会
    • Related Report
      2017 Research-status Report
  • [Presentation] プラズマ処理を用いたグラフェン合成におけるアウトガスの影響2017

    • Author(s)
      沖川侑揮、加藤隆一、山田貴壽、石原正統、長谷川雅考
    • Organizer
      グラフェン・酸化グラフェン合同シンポジウム
    • Related Report
      2017 Research-status Report
  • [Book] グラフェンから広がる二次元物質の新技術と応用2020

    • Author(s)
      吾郷浩樹、齋藤理一郎、Jari Kinaret、Vladimir Fal’ko、Zhongfan Liu、Young Hee Lee、黒部篤、長谷川雅考、沖川侑揮、山田貴壽、他多数
    • Total Pages
      558
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2019 Annual Research Report

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Published: 2017-04-28   Modified: 2021-02-19  

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