| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Outline of Final Research Achievements |
We examined the biological effect of cluster ion beams, which has never been used for the irradiation of biological samples, by using the spores of Bacillus subtilis as a model organism. Mono-layered spore samples were prepared on small pieces of silicon wafer by freeze drying followed by dew condensation treatment. The samples were irradiated in a vacuum chamber and the survival rate was determined. The lethal effect per number of atoms for 2MeV H2+ was slightly lower than that for 1 MeV H+, suggesting a negative cluster effect regarding a lethal effect. The whole genome sequencing analysis of the irradiated spores suggested that 1 MeV H+ and 2MeV H2+ may have some difference in the characteristics of induced mutations.
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