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Experimental examination for contributiions from the protein flluctuations with large amplitude and low frequencies to cooperativity of human hemoglobin

Research Project

Project/Area Number 17K05606
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological physics/Chemical physics/Soft matter physics
Research InstitutionUniversity of Tsukuba

Principal Investigator

Nagatomo Shigenori  筑波大学, 数理物質系, 講師 (80373190)

Co-Investigator(Kenkyū-buntansha) 北川 禎三  兵庫県立大学, 生命理学研究科, 客員研究員(研究員) (40029955)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsヘモグロビン / 揺らぎ / 酸素親和性 / 鉄ヒスチジン配位結合 / テラヘルツ分光 / 低波数振動 / α鎖 / 非等価 / heterogeneity / Fe-His結合 / αサブユニット / βサブユニット / 三次構造変化 / 四量体 / 協同性 / 生物物理 / 生体分子 / タンパク質 / 振動分光 / 機能発現
Outline of Final Research Achievements

Function of proteins are considered to be caused by structural change of active site, while hypotheses that fluctuations of main chains and/or low frequencies of proteins are important for function of proteins have been proposed. To detect these signals based on absorbance, apparatus connected with vector network analyzer (GHz) and Teraherz-time domain spectroscopy (TDS) was constructed. It was suggested that fluctuations of main chains and/or low frequencies of proteins were buried and that difficult to detect their signals because of large permittivity of solvent. This funding is of very significance, because clue to detect their signals can be obtained by confining proteins to medium (pore) of low permittivity.

Academic Significance and Societal Importance of the Research Achievements

タンパク質の機能発現が従来考えられている活性部位近傍の構造のみに着目するのではなく,主鎖の揺らぎ,あるいは低波数振動がタンパク質の機能発現に寄与していることの実証を主にヒト成人ヘモグロビンを用いて検証した.ギガヘルツからテラヘルツまでの帯域でアイティファクトを排した非常に小さな吸光度差を測定することができる実験系を構築し,その結果,低波数振動を観測するには,水の大きな比誘電率を軽減する必要があることを明らかにした.本研究課題「タンパク質の機能発現における主鎖の揺らぎ,あるいは低波数振動の実験的検証」の推進において,比誘電率の小さい媒体を用いる必要性が明確になったことは大きな成果であった.

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (8 results)

All 2019 2018 2017

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] A role of heme side-chains of human hemoglobin in its function revealed by circular dichroism and resonance Raman spectroscopy2018

    • Author(s)
      M. Nagai, N. Mizusawa, T. Kitagawa, S. Nagatomo
    • Journal Title

      Biophys. Rev. (Biophysical Reviews)

      Volume: 10 Issue: 2 Pages: 271-284

    • DOI

      10.1007/s12551-017-0364-5

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Heterogeneity between Two α Subunits of α2β2 Human Hemoglobin and O2 Binding Properties: Raman, 1H Nuclear Magnetic Resonance, and Terahertz Spectra2017

    • Author(s)
      Shigenori Nagatomo, Kazuya Saito, Kohji Yamamoto, Takashi Ogura, Teizo Kitagawa, and Masako Nagai
    • Journal Title

      Biochemistry

      Volume: 56 Issue: 46 Pages: 6125-6136

    • DOI

      10.1021/acs.biochem.7b00733

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] チロシン配位の天然の変異ヘモグロビンで観測される帰属未定の振動バンドの挙動と性質2019

    • Author(s)
      長友重紀,寺田拓人,庄司光男,重田育照,中谷清治,廣田俊,長井雅子,北川禎三
    • Organizer
      第46回生体分子科学討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ヒト成人ヘモグロビンの酸素親和性制御に関連したGHz, THz領域振動の研究2019

    • Author(s)
      Shigenori Nagatomo, Kohji Yamamoto, Masako Nagai, Teizo Kitagawa
    • Organizer
      日本生物物理学会第57回年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Different roles between α and β subunits in α2β2 tetramer for cooperative O2 binding of hemoglobin2018

    • Author(s)
      Shigenori Nagatomo, Takashi Ogura, Teizo Kitagawa, Masako Nagai
    • Organizer
      Tenth International Conference on Porphyrins & Phthalocyanines (ICPP-10)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ヒト成人ヘモグロビンの四量体構造の安定性に対するβサブユニットのFe-His結合の寄与2018

    • Author(s)
      長友重紀,長井雅子,北川禎三
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] ヒト成人ヘモグロビンのα、βそれぞれのサブユニットのFe-His結合と酸素親和性との関係2017

    • Author(s)
      長友重紀,奥村美紀,齋藤一弥,小倉尚志,北川禎三,長井雅子
    • Organizer
      第44回生体分子科学討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] ヘモグロビンの酸素親和性制御に関係する大振幅ヘリックス揺らぎの実験的検証:テラヘルツ(THz)分光による研究2017

    • Author(s)
      長友重紀,山本晃司,長井雅子,北川禎三
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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